Matrix stiffness regulates traction forces, cytoskeletal dynamics, and collagen reorganization in trabecular meshwork cells in glaucoma

Matter, Год журнала: 2025, Номер unknown, С. 102094 - 102094

Опубликована: Апрель 1, 2025

Язык: Английский

Gelatin sponge patch grafting of microcryogel-based three-dimensional mesenchymal stem cells to alleviate acute liver failure

Biomaterials, Год журнала: 2025, Номер unknown, С. 123324 - 123324

Опубликована: Апрель 1, 2025

The clinical application of human umbilical cord mesenchymal stem cells (hUCMSCs) in the treatment liver failure faces challenges due to cell quality, short engraftment time, and limited efficacy. Here, gelatin microcryogel (GM) microcarriers with pore sizes ranging from 15 36 μm were tuned mixed glutaraldehyde develop a 3D culture system hUCMSCs improved therapeutic effects. Bulk RNA sequencing vitro assays showed that 3D-hUCMSCs exhibited significant improvement signaling pathways related paracrine secretion anti-inflammation. These superior compared 2D-hUCMSCs not only terms secretion, protection oxidation, resistance mechanical force damage, but also had better function effect than when they transplanted as single into injury mice. Furthermore, sponge patch grafting (GSPG) strategy was developed enable direct within GM microcarriers. results overall host significantly improved, life span hosts extended. Our study provided practical achieve high long retraining time rescuing acute matrixes.

Язык: Английский

3D Traction Force Microscopy in Human Trabecular Meshwork Tissues: Effects of ROCK and YAP/TAZ Inhibition in Normal and Glaucomatous Tissues

Tissue and Cell, Год журнала: 2025, Номер unknown, С. 103005 - 103005

Опубликована: Июнь 1, 2025

Язык: Английский