Injectable strontium-containing tricalcium silicate biocements reinforced by nano HA/F127 hydrogel: Enhanced hydration kinetics, anti-washout, and dentinogenesis for vital pulp preservation

Materials Today Chemistry, Год журнала: 2025, Номер 43, С. 102503 - 102503

Опубликована: Янв. 1, 2025

Язык: Английский

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Odontogenic exosomes simulating the developmental microenvironment promote complete regeneration of pulp-dentin complex in vivo

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Establishing an optimized regenerative microenvironment for pulp-dentin complex engineering has become increasingly critical. Recently, exosomes have emerged as favorable biomimetic nanotherapeutic tools to simulate the developmental and facilitate tissue regeneration. This study aimed elucidate multifaceted roles of from human dental pulp stem cells (DPSCs) that initiated odontogenic differentiation while sustaining mesenchymal cell (MSC) characteristics in odontogenesis, angiogenesis, neurogenesis during Differential centrifugation was performed isolate normal DPSCs (DPSC-Exos) initially triggered (DPSC-Od-Exos). The impact these on biological behavior umbilical vein endothelial (HUVECs) examined vitro through CCK-8 assay Transwell migration assay, well assays dedicated assessing odontogenic, angiogenic, neurogenic capabilities. In vivo, Matrigel plugs tooth root fragments incorporating either DPSC-Exos or DPSC-Od-Exos were subcutaneously transplanted into mouse models. Subsequent histological, immunohistochemical, immunofluorescent analyses conducted determine outcomes. revealed no remarkable difference their characteristics. indicated significantly facilitated proliferation, migration, multilineage compared with DPSC-Exos. Furthermore, elicited a more pronounced effect tubular structure formation HUVECs. Consistently, plug confirmed exhibited superior performance promoting stimulating angiogenesis Notably, contributed complete regeneration fragments, characterized by enriched neurovascular structures continuous layer odontoblast-like cells, which extended cytoplasmic projections newly formed dentinal tubules. By simulating microenvironment, multifunctional demonstrated promising potential reconstructing dentin-like tissue, vascular networks, neural architectures, thereby enhancing our understanding therapeutic implications endodontic treatment.

Язык: Английский

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Extracellular matrix promotes diabetic oral wound healing by modulating the microenvironment

Biomaterials Research, Год журнала: 2025, Номер 29

Опубликована: Янв. 1, 2025

Oral wounds in diabetes mellitus (DM) often delay healing due to reduced angiogenesis and increased inflammatory response the local microenvironment, even leading graft necrosis implant failure. Therefore, developing an effective program promote is of great clinical value. Much current research focused on promoting wound through surface adhesive materials that exert a pro-angiogenic, anti-inflammatory effect. However, application bonding oral cavity very limited humid friction-prone environment. Decellularized extracellular adipose tissue (DAT) easily accessible biocompatible material derived from tissue. To further explore potential DAT, we used multi-omics analyze its composition possible mechanisms. Proteomic studies revealed DAT contains anti-inflammatory, pro-angiogenic proteins DM regeneration. adapt moist chewing friction environment mouth, modified into temperature-sensitive hydrogel can be injected intramucosally. has been verified effects macrophage phenotypic transformation. Meanwhile, transcriptome analysis suggested inhibitory effect interleukin 17 signaling pathway might key factor healing. In conclusion, after multi-omic analysis, good adapted specific cavity. This provides way setting.

Язык: Английский

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Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals

Materials Today Bio, Год журнала: 2024, Номер 26, С. 101102 - 101102

Опубликована: Май 27, 2024

Regenerative endodontic therapy is a promising approach to restore the vitality of necrotic teeth, however, pulp regeneration in mature permanent teeth remains substantial challenge due insufficient developmental signals. The dentin embryologically and histologically similar pulp, which contains cocktail pulp-specific structural proteins growth factors, thus we proposed an optimizing strategy obtain matrix extracted (DMEP) engineered DMEP functionalized double network hydrogel, whose physicochemical property was tunable by adjusting polymer concentrations synchronize with regenerated tissues.

Язык: Английский

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ADM-Loaded GelMA hydrogel promotes endogenous dental pulp regeneration: an in vitro and in vivo study

Journal of Endodontics, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

To prepare a gelatin methacryloyl (GelMA) hydrogel scaffold embedded with adrenomedullin (ADM) and investigate its impact underlying mechanisms in endogenous pulp regeneration. ADM was evenly distributed within the GelMA through simple conventional physical mixing technique. The underwent characterization via scanning electron microscopy, alongside assessments of swelling, degradation, release properties. Biocompatibility evaluated using cytoskeletal live-dead staining techniques. hydrogel's influence on dental stem cells' proliferation, migration, differentiation assessed CCK-8 assays, Transwell alizarin red alkaline phosphatase staining. Transcriptomics provided insights into potential mechanisms. angiogenic effects umbilical vein endothelial cells were examined scratch tube formation assays. In vivo, composite regenerative capacity tested rat model Statistical analysis involved Student's t-test one-way variance, significance set at P < .05. ADM-loaded (GelMA@ADM) displayed porous architecture under microscopy conducive to cell adhesion demonstrated excellent biocompatibility. vitro experiments showed that GelMA@ADM significantly boosted differentiation, enhanced activity after one week treatment. Corresponding vivo revealed facilitated new vascularized tissue 2 weeks effectively promotes proliferation augments vascularization by cells, fosters creation tissue. These findings underscore for

Язык: Английский

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Angiogenic and neurogenic potential of dental‐derived stem cells for functional pulp regeneration: A narrative review

International Endodontic Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Dental pulp tissue engineering is expected to become an ideal treatment for irreversible pulpitis and apical periodontitis. However, angiogenesis neurogenesis functional regeneration have not yet met the standard large-scale clinical application, need further research.

Язык: Английский

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