Multidirectional alignment of collagen fibers to guide cell orientation in 3D-printed tissue DOI Creative Commons
Diya Singhal, Fotis Christakopoulos, Lucia G. Brunel

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 25, 2025

Abstract Natural tissue comprises fibrous proteins with complex fiber alignment patterns. Here, we develop a reproducible method to fabricate biomimetic scaffolds patterned along multiple orientations. While extrusion-based approaches are commonly used align polymers in single orientation parallel the direction of flow, hypothesized that 3D printing could be utilized achieve more patterns alignment. Specifically, show control lateral spreading printed filament can induce is either or perpendicular flow direction. Theoretical prediction parameters was experimentally validated using collagen biomaterial ink. The velocity ratio printhead movement relative ink extrusion rate found dictate alignment, allowing for informed fabrication prescribed For example, controlled variation during print resulted specified regions both and Human corneal mesenchymal stromal cells seeded onto adopted spread morphology aligned underlying This technique worked well filaments into air extruded within support bath embedded printing, enabling structures fibers. Taken together, this work demonstrates theoretical experimental framework guide cellular

Язык: Английский

Reinforcement of Fibrillar Collagen Hydrogels with Bioorthogonal Covalent Crosslinks DOI
Lucia G. Brunel,

Chris M. Long,

Fotis Christakopoulos

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 19, 2025

Abstract Bioorthogonal covalent crosslinking stabilizes collagen type I hydrogels, improving their structural integrity for tissue engineering applications with encapsulated living cells. The chemical modification required crosslinking, however, interferes the fibrillar nature of collagen, leading instead to an amorphous network without fibers. We demonstrate approach perform bioconjugation chemistry on controlled localization such that modified retains its ability self-assemble into a network, while also displaying functional groups bioorthogonal click chemistry. matrix is formed through sequential process, in which first physically assembles fibers and then covalently crosslinked. This preserves fibrous architecture guiding behavior human corneal mesenchymal stromal cells, reinforcing crosslinks, strengthening stability cell-laden hydrogel against cell-induced contraction enzymatic degradation.

Язык: Английский

Процитировано

0
Hydrogels with multiple RGD presentations increase cell adhesion and spreading DOI Creative Commons
Abolfazl Salehi Moghaddam,

Katelyn Dunne,

Wendy A. Breyer

и другие.

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

A key challenge in hydrogel design for cell culture is replicating the cell-matrix interactions found tissues. Cells use integrins to bind their local matrix and form adhesions which dynamically move on membrane while applying significant forces matrix. Identifying important biomaterial features these challenging because it difficult independently adjust variables such as stiffness, stress relaxation, mobility of adhesion ligands, ability ligands support cellular forces. In this work, we designed a platform consisting interpenetrating polymer networks covalently crosslinked poly(ethylene glycol) (PEG) self-assembled peptide amphiphiles (PA). We can tune viscoelasticity by modulating composition both networks. Ligand be adjusted mechanical properties attaching arginine-glycine-aspartic acid (RGD) ligand either covalent PEG network, dynamic PA or at once. find that endothelial formation spreading maximized soft gels are present non-covalent The nature domains, coupled with exert substantial matrix, suggests having different presentations RGD mobile capable withstanding needed mimic aspects complex adhesions. These results will contribute hydrogels better recapitulate physiological interactions. STATEMENT OF SIGNIFICANCE: Creating artificial environments accurately how cells interact surrounding natural tissues remains fundamental biomaterials science. This study introduces dual-network controls combining stable body work has shown spreading. Our builds showing function optimally when they simultaneously engage sites force-resistant anchoring points, independent viscoelasticity. insights guide more physiologically relevant tissue engineering applications disease modeling.

Язык: Английский

Процитировано

0
Preparation, characterization, multidimensional applications and prospects of protein bio-based hydrogels: A review DOI
Tianhao Zhang, Ruihan Zhang, Chunshun Zhao

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 144199 - 144199

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Heparinization of triple-layer electrospun PLA-COL@PLA-PCL@PCL-GEL small-caliber vascular scaffold with higher anticoagulant performance DOI
Yang Chen,

Chuyao Ni,

Zihao Zhou

и другие.

Applied Physics A, Год журнала: 2025, Номер 131(6)

Опубликована: Май 17, 2025

Язык: Английский

Процитировано

0
Collagen fiber density observed in metastatic ovarian cancer promotes tumor cell adhesion DOI
Ali Abbaspour,

Ana Cavazos,

Roshan M. Patel

и другие.

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Multidirectional alignment of collagen fibers to guide cell orientation in 3D-printed tissue DOI Creative Commons
Diya Singhal, Fotis Christakopoulos, Lucia G. Brunel

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 25, 2025

Abstract Natural tissue comprises fibrous proteins with complex fiber alignment patterns. Here, we develop a reproducible method to fabricate biomimetic scaffolds patterned along multiple orientations. While extrusion-based approaches are commonly used align polymers in single orientation parallel the direction of flow, hypothesized that 3D printing could be utilized achieve more patterns alignment. Specifically, show control lateral spreading printed filament can induce is either or perpendicular flow direction. Theoretical prediction parameters was experimentally validated using collagen biomaterial ink. The velocity ratio printhead movement relative ink extrusion rate found dictate alignment, allowing for informed fabrication prescribed For example, controlled variation during print resulted specified regions both and Human corneal mesenchymal stromal cells seeded onto adopted spread morphology aligned underlying This technique worked well filaments into air extruded within support bath embedded printing, enabling structures fibers. Taken together, this work demonstrates theoretical experimental framework guide cellular

Язык: Английский

Процитировано

0