Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway DOI Creative Commons

Jianxin Yang,

Haojie Zhang,

Libin Ni

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 25, 2025

Perforator flap transplantation is an important technique in reconstructive surgery, but necrosis limits its clinical effectiveness. Thymoquinone (TQ), a natural bioactive plant quinone found black seed, exhibits anti-inflammatory, angiogenic, and antimicrobial properties. This study investigates the therapeutic effects of TQ perforator model through vivo vitro experiments. Human umbilical vein endothelial cells (HUVECs) were treated with Tert-butyl Hydroperoxide (TBHP) to simulate then TQ. In experiments used rat model, vascularization was assessed using Doppler ultrasound on days 3 7 after creation. On day post-surgery, samples collected evaluate vascularity, reactive oxygen species, apoptosis pyroptosis. Network pharmacology analysis conducted identify relevant signaling pathways, molecular docking techniques predict potential target binding sites. results showed that both treatment NLRP3 inhibitors reduced expression pyroptosis-related proteins. indicated TQ-treated group had increased survival area, blood flow intensity, microvascular density, while oxidative stress, apoptosis, pyroptosis levels reduced. Angiogenesis enhanced, SIRT1 protein increased, p-P65/NF-κB/NLRP3 pathway downregulated. After inhibitor, rate angiogenesis These findings suggest improves by inhibiting NF-κB/NLRP3 promoting angiogenesis.

Язык: Английский

Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway DOI Creative Commons

Jianxin Yang,

Haojie Zhang,

Libin Ni

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 25, 2025

Perforator flap transplantation is an important technique in reconstructive surgery, but necrosis limits its clinical effectiveness. Thymoquinone (TQ), a natural bioactive plant quinone found black seed, exhibits anti-inflammatory, angiogenic, and antimicrobial properties. This study investigates the therapeutic effects of TQ perforator model through vivo vitro experiments. Human umbilical vein endothelial cells (HUVECs) were treated with Tert-butyl Hydroperoxide (TBHP) to simulate then TQ. In experiments used rat model, vascularization was assessed using Doppler ultrasound on days 3 7 after creation. On day post-surgery, samples collected evaluate vascularity, reactive oxygen species, apoptosis pyroptosis. Network pharmacology analysis conducted identify relevant signaling pathways, molecular docking techniques predict potential target binding sites. results showed that both treatment NLRP3 inhibitors reduced expression pyroptosis-related proteins. indicated TQ-treated group had increased survival area, blood flow intensity, microvascular density, while oxidative stress, apoptosis, pyroptosis levels reduced. Angiogenesis enhanced, SIRT1 protein increased, p-P65/NF-κB/NLRP3 pathway downregulated. After inhibitor, rate angiogenesis These findings suggest improves by inhibiting NF-κB/NLRP3 promoting angiogenesis.

Язык: Английский

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