Stem Cell Research & Therapy,
Год журнала:
2023,
Номер
14(1)
Опубликована: Ноя. 14, 2023
Abstract
Background
Acute
compartment
syndrome
(ACS)
is
one
of
the
most
common
complications
musculoskeletal
injury,
leading
to
necrosis
and
demise
skeletal
muscle
cells.
Our
previous
study
showed
that
embryonic
stem
cells-derived
mesenchymal
cells
(ESC–MSCs)
are
novel
therapeutics
in
ACS
treatment.
As
extracellular
vesicles
(EVs)
rapidly
gaining
attention
as
cell-free
have
advantages
over
parental
cells,
therapeutic
potential
mechanisms
EVs
from
ESC–MSCs
on
need
be
explored.
Method
In
present
study,
we
examined
protective
effects
experimental
rat
model
investigated
role
macrophages
mediating
these
effects.
Next,
used
transcriptome
sequencing
explore
by
which
ESC–MSC-EVs
regulate
macrophage
polarization.
Furthermore,
miRNA
was
performed
identify
candidates
associated
with
Results
We
found
intravenous
administration
ESC–MSC-EVs,
given
at
time
fasciotomy,
significantly
promotes
anti-inflammation
process,
angiogenesis,
functional
recovery
ACS.
The
beneficial
were
affecting
polarization
delivering
various
miRNAs
NF-κB,
JAK/STAT,
PI3K/AKT
pathways.
data
further
illustrate
mainly
modulate
via
miR-21/PTEN,
miR-320a/PTEN,
miR-423/NLRP3,
miR-100/mTOR,
miR-26a/TLR3
axes.
Conclusion
Together,
our
results
demonstrated
ACS,
wherein
macrophages.
Journal of Extracellular Vesicles,
Год журнала:
2022,
Номер
11(1)
Опубликована: Янв. 1, 2022
Abstract
Major
depressive
disorder
(MDD)
is
the
most
prevalent
psychiatric
worldwide
and
severely
limits
psychosocial
function
quality
of
life,
but
no
effective
medication
currently
available.
Circular
RNAs
(circRNAs)
have
been
revealed
to
participate
in
MDD
pathological
process.
Targeted
delivery
circRNAs
without
blood‐brain
barrier
(BBB)
restriction
for
remission
represents
a
promising
approach
antidepressant
therapy.
In
this
study,
RVG‐circDYM‐extracellular
vesicles
(RVG‐circDYM‐EVs)
were
engineered
target
preferentially
transfer
circDYM
brain,
effect
on
process
chronic
unpredictable
stress
(CUS)
mouse
model
depression
was
investigated.
The
results
showed
that
RVG‐circDYM‐EVs
successfully
purified
by
ultracentrifugation
from
overexpressed
HEK
293T
cells,
characterization
demonstrated
terms
size,
morphology
specific
markers.
Beyond
demonstrating
proof‐of‐concept
an
RNA
drug
technology,
we
observed
systemic
administration
efficiently
delivered
alleviated
CUS‐induced
depressive‐like
behaviours,
discovered
notably
inhibited
microglial
activation,
BBB
leakiness
peripheral
immune
cells
infiltration,
attenuated
astrocyte
disfunction
induced
CUS.
CircDYM
can
bind
mechanistically
transcription
factor
TAF1
(TATA‐box
binding
protein
associated
1),
resulting
decreased
expression
its
downstream
genes
with
consequently
suppressed
neuroinflammation.
Taken
together,
our
findings
suggest
extracellular
vesicle‐mediated
treatment
clinical
applications.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Июль 20, 2023
Abstract
Extracellular
vesicles
(EVs)
are
nano-sized,
natural,
cell-derived
that
contain
the
same
nucleic
acids,
proteins,
and
lipids
as
their
source
cells.
Thus,
they
can
serve
natural
carriers
for
therapeutic
agents
drugs,
have
many
advantages
over
conventional
nanocarriers,
including
low
immunogenicity,
good
biocompatibility,
blood
–
brain
barrier
penetration,
capacity
gene
delivery.
This
review
first
introduces
classification
of
EVs
then
discusses
several
currently
popular
methods
isolating
purifying
EVs,
EVs-mediated
drug
delivery,
functionalization
carriers.
Thereby,
it
provides
new
avenues
development
EVs-based
strategies
in
different
fields
medicine.
Finally,
highlights
some
challenges
future
perspectives
with
regard
to
clinical
application
EVs.
Graphical
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Окт. 17, 2022
MicroRNAs
(miRNAs)
are
a
class
of
short,
single-stranded,
noncoding
RNAs,
with
length
about
18–22
nucleotides.
Extracellular
vesicles
(EVs)
derived
from
cells
and
play
vital
role
in
the
development
diseases
can
be
used
as
biomarkers
for
liquid
biopsy,
they
carriers
miRNA.
Existing
studies
have
found
that
most
functions
miRNA
mainly
realized
through
intercellular
transmission
EVs,
which
protect
sort
miRNAs.
Meanwhile,
detection
sensitivity
specificity
EV-derived
higher
than
those
conventional
serum
biomarkers.
In
recent
years,
EVs
been
expected
to
become
new
marker
biopsy.
This
review
summarizes
progress
several
aspects
including
sorting
mechanisms,
diagnostic
value,
technology
isolation
addition,
study
reviews
challenges
future
research
avenues
field
providing
basis
application
miRNAs
disease
clinical
diagnosis
even
point-of-care
testing
(POCT)
platforms.
Journal of Controlled Release,
Год журнала:
2023,
Номер
356, С. 463 - 480
Опубликована: Март 15, 2023
Complexity
and
difficulties
in
wound
management
are
pressing
concerns
that
affect
patients'
quality
of
life
may
result
tissue
infection,
necrosis,
loss
local
systemic
functions.
Hence,
novel
approaches
to
accelerate
healing
being
actively
explored
over
the
last
decade.
Exosomes
as
important
mediators
intercellular
communications
promising
natural
nanocarriers
due
their
biocompatibility,
low
immunogenicity,
drug
loading
targeting
capacities,
innate
stability.
More
importantly,
exosomes
developed
a
versatile
pharmaceutical
engineering
platform
for
repair.
This
review
provides
an
overview
biological
physiological
functions
derived
from
variety
origins
during
phases,
strategies
exosomal
engineering,
therapeutic
applications
skin
regeneration.
Abstract
In
the
past
decade,
nucleic
acid‐based
drugs
have
emerged
as
an
extremely
promising
approach
for
silencing
specific
disease‐related
genes
and
targeting
undruggable
ones.
However,
most
acid
drug
therapies
not
advanced
to
clinical
practice
due
their
poor
stability
against
serum
enzyme
degradation
cytotoxicity.
Nanoscale
delivery
vehicles
show
potential
improve
efficacy
of
via
targeted
disease‐causing
genes,
yet,
safe
efficient
nanocarriers
remain
elusive.
Lipid‐based
nanoparticles
such
liposomes
(LSs)
extracellular
vesicles
(EVs)
are
among
extensively
exploited
nanoscale
therapeutic
cargo
delivery.
LS‐based
been
used
several
years,
with
many
already
adopted
clinic.
More
recently,
EVs
hold
considerable
promise
high
biocompatibility,
low
immunogenicity,
inherent
abilities
interact
target
cells
cross
biological
barriers.
Moreover,
a
novel
LS/EV
hybrid
gene
system
has
engineered
preserve
benefits
both
systems
generate
system.
The
current
review
focuses
specifically
on
LS‐
EV‐based
provides
key
advantages
shortcomings
these
particular
emphasis
use
vectors.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 7333 - 7333
Опубликована: Июль 4, 2024
Clustered
regularly
interspersed
short
palindromic
repeats
(CRISPR)/CRISPR-associated
protein
9
(Cas9)
technology
has
revolutionized
the
field
of
gene
therapy
as
it
enabled
precise
genome
editing
with
unprecedented
accuracy
and
efficiency,
paving
way
for
clinical
applications
to
treat
otherwise
incurable
genetic
disorders.
Typically,
requires
delivery
multiple
components
target
cells
that,
depending
on
platform
used,
may
include
messenger
RNA
(mRNA),
complexes,
DNA
fragments.
For
purposes,
these
have
be
efficiently
delivered
into
transplantable
cells,
such
primary
T
lymphocytes
or
hematopoietic
stem
progenitor
that
are
typically
sensitive
exogenous
substances.
This
challenge
limited
broad
applicability
those
strategies
which
efficient
methods
available.
Electroporation-based
methodologies
been
generally
applied
applications,
but
procedure-associated
toxicity
represented
a
major
burden.
With
advent
novel
less
disruptive
deliver
cargo
is
now
possible
safely
editing,
thus
expanding
strategies.
In
this
review,
we
describe
different
systems
available
components,
including
viral
non-viral
systems,
highlighting
their
advantages,
limitations,
recent
applications.
Recent
improvements
achieve
cell
specificity
represent
critical
development
enable
in
vivo
targeting
future
will
certainly
play
pivotal
role
field.
Cellular and Molecular Neurobiology,
Год журнала:
2024,
Номер
44(1)
Опубликована: Фев. 19, 2024
Abstract
Neuroinflammation
is
an
important
pathogenesis
of
neurological
diseases
and
causes
a
series
physiopathological
changes,
such
as
abnormal
activation
glial
cells,
neuronal
degeneration
death,
disruption
the
blood‒brain
barrier.
Therefore,
modulating
inflammation
may
be
therapeutic
tool
for
treating
diseases.
Mesenchymal
stem
cells
(MSCs),
pluripotent
have
great
potential
due
to
their
regenerative
ability,
immunity,
ability
regulate
inflammation.
However,
recent
studies
shown
that
MSC-derived
exosomes
(MSC-Exos)
play
major
role
in
this
process
key
neuroprotection
by
regulating
neuroglia.
This
review
summarizes
progress
made
neuroinflammation
focusing
on
mechanisms
which
MSC-Exos
are
involved
regulation
through
signaling
pathways
TLR,
NF-κB,
MAPK,
STAT,
NLRP3
provide
some
references
subsequent
research
therapy.
Graphical
Exosomes
derived
from
MSCs
exhibit
neuroprotective
effects
mitigating
triggered
cells.
Journal of Nanobiotechnology,
Год журнала:
2021,
Номер
19(1)
Опубликована: Июнь 30, 2021
Abstract
Background
Extracellular
vesicles
(EVs)
secreted
by
mesenchymal
stem
cells
(MSCs)
may
play
a
vital
role
in
variety
of
biological
processes,
including
cartilage
regeneration.
However,
few
studies
reported
their
potential
the
development
osteoarthritis
(OA)
previously.
In
this
study,
we
explored
roles
and
underlying
mechanism
MSCs-EVs
OA.
Results
Co-culture
experiments
revealed
that
could
promote
expression
collagen
type
II
alpha
1
chain
(COL2A1),
SRY-box
transcription
factor
9
(SOX9)
Aggrecan
while
negatively
regulate
chondrocyte
hypertrophy
markers
matrix
metallopeptidase
13
(MMP-13)
RUNX
family
2
(Runx2)
mouse
chondrocytes
OA
model.
Besides,
results
cell
indicated
notably
weaken
suppression
proliferation,
migration
promotion
apoptosis
via
interleukin1β
(IL-1β)
induction.
addition,
MSCs-circHIPK3-EVs
(EVs
derived
from
MSCs
overexpressing
circHIPK3)
considerably
improved
IL-1β-induced
injury.
Mechanistically,
elucidated
circHIPK3
directly
bind
to
miR-124-3p
subsequently
elevate
target
gene
MYH9.
Conclusion
The
findings
our
study
demonstrated
EVs-circHIPK3
participated
MSCs-EVs-mediated
proliferation
induction
inhibition
miR-124-3p/MYH9
axis.
This
offers
promising
novel
cell-free
therapy
for
treating
Graphic
abstract
