Archives of Microbiology, Год журнала: 2024, Номер 206(7)
Опубликована: Июль 1, 2024
Язык: Английский
Archives of Microbiology, Год журнала: 2024, Номер 206(7)
Опубликована: Июль 1, 2024
Язык: Английский
Journal of Fungi, Год журнала: 2025, Номер 11(3), С. 236 - 236
Опубликована: Март 19, 2025
Biofilms are structurally organized communities of microorganisms that adhere to a variety surfaces. These produce protective matrices consisting polymeric polysaccharides, proteins, nucleic acids, and/or lipids promote shared resistance various environmental threats, including chemical, antibiotic, and immune insults. While algal bacterial biofilms more apparent in the scientific zeitgeist, many fungal pathogens also form biofilms. surprisingly common morphologically distinct from multicellular molds mushrooms normally associated with fungi instead an assemblage single-celled organisms. As collection yeast filamentous cells cloaked extracellular matrix, extreme threat public health, especially conjunction surgical implants. The encapsulated yeast, Cryptococcus neoformans, is opportunistic pathogen causes both pulmonary disseminated infections, particularly immunocompromised individuals. However, there emerging trend cryptococcosis among otherwise healthy C. neoformans forms diverse environments, within human hosts. Notably, biofilm association correlates increased expression multiple virulence factors host defenses antifungal treatments. Thus, it crucial develop novel strategies combat In this review, we discuss development treatment biofilms, particular focus on neoformans.
Язык: Английский
Процитировано
0Letters in Applied Microbiology, Год журнала: 2025, Номер 78(4)
Опубликована: Март 25, 2025
Current therapeutic alternatives for the treatment of cryptococcosis are scarce, highly toxic, expensive and difficult to access. Therefore, aim this study was evaluate anticryptococcal potential a collection 27 drugs in vitro against several strains Cryptococcus neoformans gattii. We investigated parameters antifungal activity drugs: determination minimum inhibitory concentration (MIC), combinatorial effects with fluconazole, kinetics growth inhibition, post effect (PAFE) morphometric analyses at subinhibitory concentrations. Antiparasitics albendazole, fenbendazole, flubendazole, mebendazole antidepressants duloxetine paroxetine showed MIC 100 µmol L-1 or less most tested. The results zero-interaction power model indicated additive combination fluconazole finasteride, hydroxyzine paroxetine. combined treatments significantly improved ability kill C. ATCC H99. Same phenomenon occurred PAFE, as combinations suppressed fungal more effectively than alone. A significant reduction capsule size observed. Screening drug interesting results, benzimidazoles antiparasitics serotonin norepinephrine reuptake inhibitors foreground. Finasteride, vitro.
Язык: Английский
Процитировано
0Journal of Fungi, Год журнала: 2025, Номер 11(5), С. 370 - 370
Опубликована: Май 12, 2025
With rising multidrug-resistant yeast pathogens, conventional antifungals are becoming less effective, urging the need for adjuvants that enhance their activity at lower doses. This study evaluated synergistic of antimicrobial peptidomimetics (TM8 and RK758) or colistin sulphate in combination with against Candida albicans, C. tropicalis, parapsilosis, Meyerozyma guilliermondii, Nakaseomyces glabratus, Pichia kudriavzevii Kluyveromyces marxianus, Candidozyma auris using checkerboard microdilution test. RK758 was fluconazole 78% isolates, remaining 22% isolates still showing partial synergy; it showed synergy amphotericin B 56% caspofungin, exhibited either synergy. TM8 44% (with another 44%) 67% caspofungin isolates. Colistin No antagonism observed any combinations. Additionally, a time-kill assay further demonstrated between RK758. The effects these on cell membrane integrity were an ergosterol binding assay, supported by SYTOX Green cellular leakage assays, both indicating lytic effect. These results suggest can synergise antifungals, offering potential strategy therapy infections. likely plays role interaction thereby enhancing activities compounds sub-MIC levels.
Язык: Английский
Процитировано
0Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Май 20, 2025
Abstract The rise of drug-resistant fungal species, such as Candida auris , poses a serious threat to global health, with mortality rates exceeding 40% and resistance surpassing 90%. limited arsenal effective antifungal agents underscores the urgent need for novel strategies. Here, we systematically evaluate role histone H3 post-translational modifications in C. drug resistance, focusing on acetylation mediated by Gcn5 Rtt109, methylation Set1, Set2, Dot1. Mutants deficient these enzymes exhibit varying degrees sensitivity. Notably, discover that GCN5 depletion subsequent loss downregulates key genes involved ergosterol biosynthesis efflux, resulting increased susceptibility azoles polyenes. Additionally, regulates cell wall integrity echinocandin through calcineurin signaling pathway transcription factor Cas5. In infection models using Galleria mellonella immunocompromised mice, deletion significantly reduces virulence . Furthermore, inhibitor CPTH 2 synergizes caspofungin vitro vivo without notable toxicity. These findings highlight critical pathogenicity positioning it promising therapeutic target combating invasive infections.
Язык: Английский
Процитировано
0Springer series on biofilms, Год журнала: 2025, Номер unknown, С. 239 - 279
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0International Journal of Pharmaceutics, Год журнала: 2023, Номер 646, С. 123471 - 123471
Опубликована: Окт. 2, 2023
Язык: Английский
Процитировано
5International Journal of Infectious Diseases, Год журнала: 2024, Номер 146, С. 107117 - 107117
Опубликована: Май 26, 2024
Язык: Английский
Процитировано
1Current Opinion in Infectious Diseases, Год журнала: 2024, Номер unknown
Опубликована: Сен. 11, 2024
Purpose of review Given the high mortality and morbidity associated with invasive fungal diseases (IFDs), use combination antifungal therapies is often considered despite dearth data. This aims to summarize current state literature therapies, discussing potential roles newer combinations key considerations for their clinical use. Recent findings In infections other than cryptococcal meningitis or in setting empirical treatment suspected azole-resistant Aspergillus infections, utility approaches remains controversial given paucity well designed randomized controlled trials. Data on combined treatments have been primarily limited in-vitro studies, animal models, case reports and/or observational studies. With availability novel agents (e.g. ibrexafungerp, fosmanogepix), therapy treat mould should be re-visited. A phase 2 trial ibrexafungerp voriconazole pulmonary aspergillosis on-going. Summary There a need investigate agents. includes delineating indication these determining how them most appropriately setting.
Язык: Английский
Процитировано
1Frontiers in Drug Delivery, Год журнала: 2024, Номер 4
Опубликована: Сен. 16, 2024
Fungal infections, though affecting healthcare globally, receive insufficient attention in clinical and academic settings. Invasive fungal particularly caused by combat wounds, have been identified as a critical threat the US Department of Defense. Monotherapy with traditional antifungals is often insufficient, so combination therapies are explored to enhance treatment efficacy. However, systemic treatments can result severe adverse effects, suggesting need for localised delivery systems, such drug-loaded electrospun patches, administer directly infection site. This proof-of-concept study hypothesised that dual amorolfine terbinafine therapy slowly releasing from patches would be an effective way eradicating Candida albicans when patch was applied colony. The feasibility creating materials loaded antifungal investigated. Electrospinning used fabricate polycaprolactone (PCL) varying drug loadings (2.5%, 5%, 10% w/w) either individually or combination. incorporation both drugs fibres confirmed, predominantly amorphous state. Results showed had significantly greater prolonged effect compared monotherapy larger zones inhibition sustained efficacy over at least 7 days. therefore demonstrates PCL-based containing provide superior activity against C. patches. approach could lower required doses, reducing patient compliance due release, leading more therapy.
Язык: Английский
Процитировано
1Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Ноя. 21, 2024
Язык: Английский
Процитировано
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