Nanotechnological advancement for the treatment of neurodegenerative disorders

Journal of Dispersion Science and Technology, Год журнала: 2024, Номер unknown, С. 1 - 19

Опубликована: Ноя. 25, 2024

Язык: Английский

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Antifungal activity of bamemacrolactine C against Talaromyces marneffei and its possible mechanisms of action

Journal of Applied Microbiology, Год журнала: 2024, Номер 135(12)

Опубликована: Дек. 1, 2024

Abstract Aims The present study aims to investigate the in vitro antifungal activity and mechanism of action bamemacrolactine C (BAC), a new 24-membered macrolide compound, against Talaromyces marneffei. Methods results test drug BAC initially demonstrated through paper disk diffusion assay, followed by determination minimum inhibitory concentration value 35.29 μg ml−1 using microdilution. association revealed that combination therapy exhibited additive effects (0.5 < FICI 1.0) when combined with either amphotericin B or fluconazole. A time-growth assay confirmed treatment completely inhibited growth T. marneffei effects. Micromorphological analysis scanning electron microscopy transmission photomicrographs induced morphological damage fungal cells compared control group. Transmembrane protein assays showed significant reduction levels Na+/K+-ATPase (P .05) Ca2+-ATPase .01) Intracellular enzyme significantly decreased ATP, malate dehydrogenase, succinate dehydrogenase content .01). proteomics parallel reaction monitoring (PRM) verification indicated exhibits an downregulating ATP citric acid lyase (ACLY) , potentially affecting tricarboxylic (TCA) cycle. Besides, binding model ACLY also shows good docking score. Conclusions findings suggest marneffei, elucidating its multifaceted involving disruption cell membranes’ integrity inhibition intracellular activities, which modulation TCA cycle may play important role.

Язык: Английский

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Synthesis of novel thiazol-2(3H)-imine derivatives as ergosterol biosynthesis inhibitors, and elucidation of their structures using a 2D NMR technique

New Journal of Chemistry, Год журнала: 2023, Номер 47(37), С. 17558 - 17566

Опубликована: Янв. 1, 2023

In this study, new imidazole-2,3-dihydrothiazole derivatives were synthesized.

Язык: Английский

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In vitro and in vivo antimicrobial effects of domiphen combined with itraconazole against Aspergillus fumigatus

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 21, 2023

Aspergillus fumigatus , a prevalent saprophytic fungus in the atmosphere, is known to rapidly induce severe invasive aspergillosis (IA) upon inhalation of its conidia by humans or animals. The mortality rate associated with IA exceeds 50%. misuse antifungal agents has contributed emergence numerous highly pathogenic drug-resistant strains A. . Our study found that combination domiphen and itraconazole had sound synergistic antimicrobial effects against wild-type itraconazole-resistant vivo vitro through MIC, FIC, plate inoculation, growth curve experiments, Galleria mellonella infection model. Drug cytotoxicity pharmacological tests for acute toxicity assays demonstrated both showed minimal good biocompatibility. transcriptome sequencing experiment exerted suppressive effect on expression various genes, including those involved drug efflux, redox regulation, cellular membrane cell wall remodeling. present investigation explores mechanisms itraconazole, encompassing three key aspects: (i) inhibited efflux reducing efflux-related (ii) ability disrupt wall, (iii) also can remove biofilm more effectively. In summary, utilization as synergist exhibited disruptive biofilm, This subsequently led modified distribution within fungal organism, ultimately resulting enhanced efficacy. results this may provide new therapeutic strategy treatment caused

Язык: Английский

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Imidazolium-based ionic liquids disrupt saccharomyces cerevisiae cell membrane integrity

Archives of Microbiology, Год журнала: 2024, Номер 206(7)

Опубликована: Июль 1, 2024

Язык: Английский

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