Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 28, 2024
Abstract
Musculoskeletal
diseases
have
emerged
as
the
leading
cause
of
disability
worldwide,
with
their
prevalence
increasing
annually.
In
light
this
escalating
health
challenge,
organoids,
an
emerging
technology
in
tissue
engineering,
offer
promising
solutions
for
disease
modeling,
drug
screening,
regeneration,
and
repair
processes.
The
successful
development
musculoskeletal
organoids
represents
a
significant
breakthrough,
providing
novel
platform
studying
facilitating
discovery
new
treatments.
Moreover,
serve
valuable
complements
to
traditional
2D
culture
methods
animal
models,
offering
rich
insights
into
biology.
This
review
provides
overview
organoid
technology,
outlining
construction
processes
various
highlighting
similarities
differences.
Furthermore,
challenges
associated
systems
are
discussed
future
perspectives
offered.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Авг. 21, 2024
Bone
defects
pose
significant
challenges
in
healthcare,
with
over
2
million
bone
repair
surgeries
performed
globally
each
year.
As
a
burgeoning
force
the
field
of
tissue
engineering,
3D
printing
offers
novel
solutions
to
traditional
transplantation
procedures.
However,
current
3D-printed
scaffolds
still
face
three
critical
material
selection,
methods,
cellular
self-organization
and
co-culture,
significantly
impeding
their
clinical
application.
In
this
comprehensive
review,
we
delve
into
performance
criteria
that
ideal
should
possess,
particular
focus
on
core
faced
by
technology
during
translation.
We
summarize
latest
advancements
non-traditional
materials
advanced
techniques,
emphasizing
importance
integrating
organ-like
technologies
bioprinting.
This
combined
approach
enables
more
precise
simulation
natural
structure
function.
Our
aim
writing
review
is
propose
effective
strategies
address
these
promote
translation
for
defect
treatment.
Biomaterials,
Год журнала:
2024,
Номер
311, С. 122681 - 122681
Опубликована: Июнь 25, 2024
Cell-laden
bioprinting
is
a
promising
biofabrication
strategy
for
regenerating
bioactive
transplants
to
address
organ
donor
shortages.
However,
there
has
been
little
success
in
reproducing
transplantable
artificial
organs
with
multiple
distinctive
cell
types
and
physiologically
relevant
architecture.
In
this
study,
an
omnidirectional
printing
embedded
network
(OPEN)
presented
as
support
medium
3D
printing.
The
state-of-the-art
due
its
one-step
preparation,
fast
removal,
versatile
ink
compatibility.
To
test
the
feasibility
of
OPEN,
exceptional
primary
mouse
hepatocytes
(PMHs)
endothelial
line-C166,
were
used
print
hepatospheroid-encapsulated-artificial
livers
(HEALs)
vein
structures
following
predesigned
anatomy-based
paths
OPEN.
PMHs
self-organized
into
hepatocyte
spheroids
within
matrix,
whereas
entire
cross-linked
structure
remained
intact
minimum
ten
days
cultivation.
Cultivated
HEALs
maintained
mature
hepatic
functions
marker
gene
expression
at
higher
level
than
conventional
2D
conditions
vitro.
C166-laden
promoted
endogenous
neovascularization
vivo
compared
hepatospheroid-only
liver
prints
two
weeks
transplantation.
Collectively,
proposed
platform
enables
manufacture
tissues
or
resembling
anatomical
architecture,
broad
implications
function
replacement
clinical
applications.
Cells,
Год журнала:
2024,
Номер
13(12), С. 1054 - 1054
Опубликована: Июнь 18, 2024
Over
the
past
decade,
development
of
three-dimensional
(3D)
models
has
increased
exponentially,
facilitating
unravelling
fundamental
and
essential
cellular
mechanisms
by
which
cells
communicate
with
each
other,
assemble
into
tissues
organs
respond
to
biochemical
biophysical
stimuli
under
both
physiological
pathological
conditions.
This
section
presents
a
concise
overview
most
recent
updates
on
significant
contribution
different
types
3D
cell
cultures
including
spheroids,
organoids
organ-on-chip
bio-printed
in
advancing
our
understanding
molecular
mechanisms.
The
case
studies
presented
include
breast
cancer
(BC),
endometriosis,
liver
microenvironment
infections.
In
BC,
establishment
culture
permitted
visualization
role
cancer-associated
fibroblasts
delivery
exosomes,
as
well
significance
physical
properties
extracellular
matrix
promoting
proliferation
invasion.
approach
also
become
valuable
tool
gaining
insight
general
specific
drug
resistance.
Given
considerable
heterogeneity
offer
more
accurate
representation
vivo
microenvironment,
thereby
identification
translation
novel
targeted
therapeutic
strategies.
advantages
provided
hepatic
environment,
conjunction
high
throughput
characterizing
various
platforms,
have
enabled
elucidation
complex
underlying
threatening
diseases.
A
limited
number
for
gut
skin
infections
been
developed.
However,
profound
comprehension
spatial
temporal
interactions
between
microbes,
host
their
environment
may
facilitate
advancement
vitro,
ex
disease
models.
Additionally,
it
pave
way
approaches
diverse
research
fields.
interested
reader
will
find
concluding
remarks
challenges
prospects
using
discovering
areas
covered
this
review.
Cells,
Год журнала:
2024,
Номер
13(19), С. 1638 - 1638
Опубликована: Окт. 1, 2024
The
3D
bioprinting
technique
has
made
enormous
progress
in
tissue
engineering,
regenerative
medicine
and
research
into
diseases
such
as
cancer.
Apart
from
individual
cells,
a
collection
of
organoids,
can
be
printed
combination
with
various
hydrogels.
It
hypothesized
that
will
even
become
promising
tool
for
mechanobiological
analyses
organoids
their
matrix
environments
highly
defined
precisely
structured
environments,
which
the
mechanical
properties
cell
environment
individually
adjusted.
Mechanical
obstacles
or
bead
markers
integrated
bioprinted
samples
to
analyze
deformations
forces
within
these
constructs,
perform
biophysical
analysis
complex
systems,
are
still
not
standard
techniques.
review
highlights
advances
4D
printing
technologies
integrating
cues
so
next
step
detailed
key
future
directions
organoid
generation
development
disease
model
regeneration
drug
testing
perspective.
Finally,
hydrogels,
pure
natural
synthetic
hydrogels
mixtures,
organoid–cell
co-cultures,
organ-on-a-chip
systems
organoid-organ-on-a
chip
combinations
introduces
use
assembloids
determine
mutual
interactions
different
types
cell–matrix
interferences
specific
biological
environments.
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 8, 2024
The
complexity
of
tumors
and
the
challenges
associated
with
treatment
often
stem
from
limitations
existing
models
in
accurately
replicating
authentic
tumors.
Recently,
organoid
technology
has
emerged
as
an
innovative
platform
for
tumor
research.
This
bioengineering
approach
enables
researchers
to
simulate,
vitro,
interactions
between
their
microenvironment,
thereby
enhancing
intricate
interplay
cells
surroundings.
Organoids
also
integrate
multidimensional
data,
providing
a
novel
paradigm
understanding
development
progression
while
facilitating
precision
therapy.
Furthermore,
advancements
imaging
genetic
editing
techniques
have
significantly
augmented
potential
organoids
review
explores
application
more
precise
simulations
its
specific
contributions
cancer
research
advancements.
Additionally,
we
discuss
evolving
trends
developing
comprehensive
utilizing
technology.
Abstract
Current
two-dimensional
(2D)
cell
models
for
effective
drug
screening
suffer
from
significant
limitations
imposed
by
the
lack
of
realism
in
physiological
environment.
Three-dimensional
(3D)
organoids
hold
immense
potential
mimicking
key
functions
human
organs
overcoming
traditional
2D
models.
However,
current
techniques
preparation
3D
had
reproducibility,
scalability,
and
ability
to
closely
replicate
complex
microenvironment
found
vivo
.
Additionally,
culture
systems
often
involve
lengthy
labor-intensive
processes
that
hinder
high-throughput
applications
necessary
a
large-scale
screening.
Advancements
bioprinting
technologies
offer
promising
solutions
these
challenges
enabling
precise
spatial
control
over
placement
material
composition,
thereby
facilitating
creation
more
physiologically
relevant
than
techniques.
This
review
provides
comprehensive
summary
recent
advances
creating
models,
which
begins
with
an
introduction
different
types
(especially
focus
on
volumetric
(VBP)
technique),
followed
overview
bioinks
utilized
bioprinting.
Moreover,
we
also
introduce
disease
efficiency
evaluation
regenerative
medicine.
Finally,
possible
strategies
development
clinical
translation
are
concluded.
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 16, 2025
Gynecologic
cancers
(GCs),
including
cervical
cancer
(CC),
ovarian
(OC),
endometrial
(EC),
as
well
vulvar
and
vaginal
cancers,
represent
major
health
threats
to
women,
with
increasing
incidence
rates
observed
globally.
Conventional
treatments,
such
surgery,
radiation
therapy,
chemotherapy,
are
often
hindered
by
challenges
drug
resistance
recurrence,
contributing
high
mortality
rates.
Organoid
technology
has
emerged
a
transformative
tool
in
research,
offering
vitro
models
that
closely
replicate
the
tumor
cell
architecture
heterogeneity
of
primary
cancers.
Tumor-derived
organoids
preserve
histological
molecular
characteristics
original
tumors,
making
them
invaluable
for
studying
biology,
pathways,
immune
microenvironment.
Furthermore,
play
crucial
role
biomarker
discovery,
screening,
development
personalized
therapeutic
strategies.
In
contrast
traditional
lines
patient-derived
xenograft
(PDX)
models,
gynecologic
accurately
mirror
genetic
mutations
specific
gene
expression
profiles
tumors.
This
review
provides
an
overview
recent
advancements
organoid
highlighting
their
contributions
understanding
disease
mechanisms,
facilitating
advancing
precision
medicine.
It
also
addresses
potential
technology,
focus
on
its
treatment
approaches
GCs.
Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials,
Год журнала:
2025,
Номер
166, С. 106949 - 106949
