Barcoded screening identifies nanocarriers for protein delivery to kidney

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 21, 2025

Targeted protein delivery with nanocarriers holds significant potential to enhance therapeutic outcomes by precisely directing proteins specific organs or tissues. However, the complex interactions between and biological environment pose considerable challenges in designing effective targeted vehicles. In this study, we address challenge leveraging DNA-barcoded high-throughput screening. We construct a nanocapsule library via in-situ polymerization, incorporating various monomers create nanocapsules unique surface properties. vitro vivo screening, using female mice, identify high cell association different biodistribution. Our investigation into kidney-enriched highlights crucial role of polymer composition biodistribution, demonstrating engineering for precise control over nanoparticle distribution. The successfully delivers catalase, showcasing its mitigating cisplatin-induced acute kidney injury. Overall, our study presents an approach identifying vehicles, capacity broaden application as agents research tools. development requires methods screening vivo. Here authors develop method biodistributions, demonstrate catalase kidneys

Язык: Английский

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A Dynamic Deferoxamine Polymer with Exceptional Performance in Mitochondrial Iron Depletion and Cytosolic Protein Delivery

Small, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

Abstract Deferoxamine (DFO) is an FDA‐approved naturally occurring iron chelator commonly used to treat transfusion‐induced overload. The abundant and flexible hydroxamic acid groups in DFO enable exceptional binding capacity high protein via hydrogen bonding interactions. However, the applications of sequester intracellular deliver proteins inside cells are limited due poor membrane‐permeability. Herein, fabrication a dynamic polymer proposed achieve robust delivery efficient mitochondrial depletion. Specifically, grafted onto polycatechol scaffold catechol‐boronate chemistry. obtained shows capacity, formed complexes show resistance toward serum proteins. It effectively delivers various cargo into cytosol treated with maintained bioactivity. In addition, cells, released efficiently depletes iron, which significantly inhibits oxidative phosphorylation glycolysis. Remarkable synergistic cytotoxic effects achieved when loaded toxic This study provides general strategy for facile preparation bioactive delivery, designed can be promising carrier therapeutics cancer.

Язык: Английский

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Host RNA-binding proteins and specialized viral RNA translation mechanisms: potential antiviral targets.

Antiviral Research, Год журнала: 2025, Номер unknown, С. 106142 - 106142

Опубликована: Март 1, 2025

Язык: Английский

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ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

The intracellular delivery of protein drugs via nanocarriers offers significant potential for expanding their therapeutic applications. However, the unintended activation innate immune responses and inflammation triggered by carriers presents a major challenge, often compromising efficacy. Here, we present oligoethylenimine-thioketal (OEI-TK), reactive oxygen species-responsive cationic polymer with intrinsic anti-inflammatory properties, to overcome this challenge. OEI-TK self-assembles electrostatically bovine serum albumin (BSA) form stable nanoparticles (OTB NPs) excellent encapsulation efficiency. In vitro studies confirmed that OTB NPs retained OEI-TK's antioxidant enhanced biocompatibility, efficiently delivered BSA into cells. Furthermore, facilitated β-galactosidase while preserving its enzymatic activity, demonstrating functional transport. These findings highlight as promising platform dual benefits modulation delivery, holding synergistic treatment inflammation-related diseases.

Язык: Английский

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Specifically targeted antimicrobial cyclic peptide to staphylococcal protein A for the treatment of S. aureus infection

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162243 - 162243

Опубликована: Апрель 1, 2025

Язык: Английский

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Editorial: Subcellular organelle-targeting of nanomaterials for enhancing therapeutic effectiveness

Advanced Drug Delivery Reviews, Год журнала: 2024, Номер unknown, С. 115460 - 115460

Опубликована: Окт. 1, 2024

Язык: Английский

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