Selective separation of dyes and tetracycline hydrochloride in polymer‐nucleotide coacervate droplets DOI
Chuntao Zhu, Meng Yu,

Zijian Liang

и другие.

Journal of Applied Polymer Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 30, 2024

Abstract This paper reports the formation of coacervates by electrostatic interaction poly (diallyldimethylammonium chloride) (PDDA) and adenosine triphosphate (ATP) in aqueous solution, examining its conditions, stability, efficiency separation. The ideal concentration for creating coacervate droplets pure water, HEPES buffer, NaCl solution was determined to be 20 mM PDDA ATP. Enhancing stability achieved incorporating phospholipid vesicles on their surface, presenting a novel strategy building cell models. Ostwald Ripening employed comprehend growth mechanism coacervates, while Hofmeister Ion Series Schulze‐Hardy's rule were utilized elucidate differences solutions containing NaCl, Na 2 SO 4 , MgCl . These stable at concentrations below 90 200 30 respectively. We also explored specific separation dyes tetracycline hydrochloride (TC) coacervates. Separation efficiencies 92.98% methylene blue (MB), 94.19% methyl orange (MO), 85.94% TC, which can attributed synergistic effects hydrophobicity, forces, π‐π interactions. proposed have great potential mimicry water treatment.

Язык: Английский

Disordered peptide-based design of intrinsically disordered polymers for biomedical applications DOI
Orkid Coskuner‐Weber,

Fatma Nilsu Cayli,

Vladimir N. Uversky

и другие.

International Journal of Polymer Analysis and Characterization, Год журнала: 2025, Номер unknown, С. 1 - 49

Опубликована: Фев. 10, 2025

Язык: Английский

Процитировано

0
Genetically-Encoded Phase Separation Sensors Enable High-Fidelity Live-Cell Probing of Biomolecular Condensates DOI Creative Commons
Alexa Regina Chua Avecilla, Jeremy A. Thomas, Felipe García Quiroz

и другие.

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Фев. 23, 2025

Biomolecular condensates are membraneless compartments with enigmatic roles across intracellular phenomena. Intrinsically disordered proteins (IDPs) often function as condensate scaffolds, fueled by liquid–liquid phase separation (LLPS) dynamics. Intracellular probing of relies on live-cell imaging IDP-scaffolds tagged fluorescent proteins. Conformational heterogeneity in IDPs, however, renders them uniquely susceptible to artifacts from tagging. Probing epidermal skin, we recently introduced genetically-encoded LLPS-sensors that circumvent the need for molecular-level tagging skin IDPs. Departing subcellular tracking IDP-scaffolds, report assembly and liquid-like dynamics their condensates. Here, demonstrate biomolecular approaches evolution tunability assess impact early late stages separation. Benchmarking against scaffold-bound reporters, discovered tunable ultraweak scaffold–sensor interactions enable sensitive innocuous nascent established Our LLPS-sensitive tools pave way high-fidelity IDP-governed biological systems.

Язык: Английский

Процитировано

0
Genetically-encoded phase separation sensors for intracellular probing of biomolecular condensates DOI Creative Commons
Alexa Regina Chua Avecilla, Jeremy A. Thomas, Felipe García Quiroz

и другие.

Опубликована: Авг. 30, 2024

Biomolecular condensates are dynamic membraneless compartments with enigmatic roles across intracellular phenomena. Intrinsically-disordered proteins (IDPs) often function as condensate scaffolds, fueled by their liquid-liquid phase separation (LLPS) dynamics. Intracellular probing of these relies on live-cell imaging IDP-scaffolds tagged fluorescent proteins. Conformational heterogeneity in IDPs, however, renders them uniquely sensitive to molecular-level fusions, risking distortion the native biophysical properties and assemblies. Probing epidermal mouse skin, we recently introduced genetically encoded LLPS-sensors that circumvent need for tagging skin IDPs. The concept involves a shift focus from subcellular tracking higher-level observations report assembly liquid-dynamics condensates. Towards advancing repertoire LLPS-sensors, here demonstrate biomolecular approaches evolution tunability assess impact early late stages LLPS Benchmarking against scaffold-bound reporters, found tunable ultraweak scaffold-sensor interactions key innocuous nascent established Our LLPS-sensitive tools pave way high-fidelity IDP-governed biological systems.

Язык: Английский

Процитировано

1
Selective separation of dyes and tetracycline hydrochloride in polymer‐nucleotide coacervate droplets DOI
Chuntao Zhu, Meng Yu,

Zijian Liang

и другие.

Journal of Applied Polymer Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 30, 2024

Abstract This paper reports the formation of coacervates by electrostatic interaction poly (diallyldimethylammonium chloride) (PDDA) and adenosine triphosphate (ATP) in aqueous solution, examining its conditions, stability, efficiency separation. The ideal concentration for creating coacervate droplets pure water, HEPES buffer, NaCl solution was determined to be 20 mM PDDA ATP. Enhancing stability achieved incorporating phospholipid vesicles on their surface, presenting a novel strategy building cell models. Ostwald Ripening employed comprehend growth mechanism coacervates, while Hofmeister Ion Series Schulze‐Hardy's rule were utilized elucidate differences solutions containing NaCl, Na 2 SO 4 , MgCl . These stable at concentrations below 90 200 30 respectively. We also explored specific separation dyes tetracycline hydrochloride (TC) coacervates. Separation efficiencies 92.98% methylene blue (MB), 94.19% methyl orange (MO), 85.94% TC, which can attributed synergistic effects hydrophobicity, forces, π‐π interactions. proposed have great potential mimicry water treatment.

Язык: Английский

Процитировано

0