Three-dimensional bioprinted gelatin methacryloyl scaffold: a versatile platform for nasal cell culture and drug testing applications DOI Creative Commons
Chun Y. Wong, Hui Xin Ong, Daniela Traini

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125803 - 125803

Опубликована: Июнь 1, 2025

Developing advanced biomaterials and precision techniques is critical for tissue engineering drug testing. This study shows the design development of a novel 3D-bioprinted gelatin methacryloyl (GelMA) scaffold embedded with RPMI-2650 nasal epithelial cells cell culture testing applications. The CAD-designed presented robust structure biocompatibility across different densities. Studies swelling behaviour displayed effective medium transport retention, especially in cell-laden scaffolds. potential GelMA to enable 3D growth was confirmed by continuously high viability (>95 %), spheroid formation substantial proliferation. Biocompatibility live/dead imaging coverage studies, which revealed 5-fold increase live over two weeks. These findings highlight scaffolds as customisable, biocompatible, platform advancing screening, culture, engineering. Future studies will focus on optimising composition incorporating dynamic conditions further enhance physiological relevance translational potential.

Язык: Английский

Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells DOI
Yuyan Huang, Liting Liang, Yi Kong

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 2, 2025

Mesenchymal stem cells (MSCs) play a critical role in cell therapy due to their tissue-mimicking abilities. However, conventional 2D culture conditions often lead the loss of native hypoxic niche, potentially limiting therapeutic efficacy. 3D bioprinting offers method recreate intricate biological environments by integrating with extracellular matrices. Therefore, it is essential adapt printing techniques accurately replicate MSCs' ecological facilitating integration technology into clinical applications. In this study, we optimized capabilities using performed cellular aggregates (PCA) method. We observed that printed matrix creates microenvironment, resulting significant increase level production several paracrine signaling molecules and immunomodulatory factors MSCs. Furthermore, MSCs exhibited enhanced stemness proliferative capacity early stages culture. RNA-seq analysis revealed these changes behavior were associated environment created during procedure By optimizing bioink composition parameters, successfully simulated vivo leading notable improvements MSC characteristics capacity. RNA sequencing further confirmed activation hypoxia pathways, which are crucial for properties. These findings offer valuable insights leveraging MSC-based therapies regenerative medicine.

Язык: Английский

Процитировано

1

The rise of 3D bioprinting: from organs to personalized medicine DOI Creative Commons
Kirolos Eskandar

Patient-Oriented Medicine and Pharmacy, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Bioprinting has emerged as a groundbreaking technology in the realms of organ transplantation and regenerative medicine, addressing critical challenges such shortages tissue repair. This review explores technological advancements innovations bioprinting, highlighting state-of-the-art techniques, bioinks, applications engineering. Key milestones printing functional tissues, including vascularized transplantable prototypes, are discussed alongside role bioprinting personalized where patient-oriented models revolutionizing drug testing therapeutic strategies. Furthermore, this article examines ethical considerations associated with offering insights into its future potential to transform global healthcare.

Язык: Английский

Процитировано

0

Natural macromolecule-based bioinks for 3D bioprinting: A systematic review of composition, physicochemical characterization, and biomedical applications DOI Creative Commons

Tatiana Muñoz-Castiblanco,

Juan P. Moreno-Marín,

Marlon Osorio

и другие.

Bioprinting, Год журнала: 2025, Номер unknown, С. e00407 - e00407

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Bioprinting in Organ Transplantation: From Experimental Models to Clinical Prospects DOI Creative Commons
Kirolos Eskandar

Brazilian Journal of Transplantation, Год журнала: 2025, Номер 28(1)

Опубликована: Апрель 25, 2025

Background: Bioprinting has emerged as an innovative technology in organ transplantation and regenerative medicine, aiming to address pressing challenges such the shortage of donor organs need for effective tissue repair. By leveraging advanced 3D printing techniques, bioprinting enables fabrication functional tissues with precise architectural biological properties. Methods: This review provides in-depth analysis latest advancements bioprinting, focusing on cutting-edge development bioinks, their applications engineering. It examines significant breakthroughs creation vascularized transplantable prototypes explores role personalized medicine. Results: The findings highlight transformative impact biomedical field, particularly drug testing, therapeutic modeling, patient-specific treatment strategies. Additionally, key challenges—including technological limitations, ethical concerns, regulatory considerations—are discussed provide a comprehensive understanding field’s progress potential obstacles. Conclusion: holds immense promise revolutionizing global healthcare by offering solutions shortages advancing However, continued research innovation are necessary overcome existing facilitate its clinical translation into mainstream medical practice.

Язык: Английский

Процитировано

0

Bioimpressão no Transplante de Órgãos: Dos Modelos Experimentais às Perspectivas Clínicas DOI Creative Commons
Kirolos Eskandar

Brazilian Journal of Transplantation, Год журнала: 2025, Номер 28(1)

Опубликована: Апрель 25, 2025

Introdução: A bioimpressão despontou como uma tecnologia inovadora no transplante de órgãos e na medicina regenerativa, visando solucionar desafios urgentes, a escassez doadores necessidade reparo eficaz tecidos. Ao aproveitar as técnicas avançadas impressão 3D, permite fabricação tecidos funcionais com propriedades arquitetônicas biológicas precisas. Métodos: Esta revisão fornece análise detalhada dos últimos avanços em bioimpressão, concentrando-se ponta, desenvolvimento biotintas suas aplicações engenharia Ela examina os significativos criação protótipos vascularizados transplantáveis explora função da personalizada. Resultados: As descobertas destacam o impacto transformador campo biomédico, particularmente testes medicamentos, modelagem terapêutica estratégias tratamento específicas para paciente. Além disso, principais — incluindo limitações tecnológicas, preocupações éticas considerações regulatórias são discutidos fornecer compreensão abrangente do progresso possíveis obstáculos. Conclusão: é imensamente promissora revolucionar saúde global, oferecendo soluções avanço regenerativa. No entanto pesquisa inovação contínuas necessárias superar existentes facilitar sua tradução clínica prática médica convencional.

Процитировано

0

Three-dimensional bioprinted gelatin methacryloyl scaffold: a versatile platform for nasal cell culture and drug testing applications DOI Creative Commons
Chun Y. Wong, Hui Xin Ong, Daniela Traini

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125803 - 125803

Опубликована: Июнь 1, 2025

Developing advanced biomaterials and precision techniques is critical for tissue engineering drug testing. This study shows the design development of a novel 3D-bioprinted gelatin methacryloyl (GelMA) scaffold embedded with RPMI-2650 nasal epithelial cells cell culture testing applications. The CAD-designed presented robust structure biocompatibility across different densities. Studies swelling behaviour displayed effective medium transport retention, especially in cell-laden scaffolds. potential GelMA to enable 3D growth was confirmed by continuously high viability (>95 %), spheroid formation substantial proliferation. Biocompatibility live/dead imaging coverage studies, which revealed 5-fold increase live over two weeks. These findings highlight scaffolds as customisable, biocompatible, platform advancing screening, culture, engineering. Future studies will focus on optimising composition incorporating dynamic conditions further enhance physiological relevance translational potential.

Язык: Английский

Процитировано

0