Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 2, 2025
Mesenchymal
stem
cells
(MSCs)
play
a
critical
role
in
cell
therapy
due
to
their
tissue-mimicking
abilities.
However,
conventional
2D
culture
conditions
often
lead
the
loss
of
native
hypoxic
niche,
potentially
limiting
therapeutic
efficacy.
3D
bioprinting
offers
method
recreate
intricate
biological
environments
by
integrating
with
extracellular
matrices.
Therefore,
it
is
essential
adapt
printing
techniques
accurately
replicate
MSCs'
ecological
facilitating
integration
technology
into
clinical
applications.
In
this
study,
we
optimized
capabilities
using
performed
cellular
aggregates
(PCA)
method.
We
observed
that
printed
matrix
creates
microenvironment,
resulting
significant
increase
level
production
several
paracrine
signaling
molecules
and
immunomodulatory
factors
MSCs.
Furthermore,
MSCs
exhibited
enhanced
stemness
proliferative
capacity
early
stages
culture.
RNA-seq
analysis
revealed
these
changes
behavior
were
associated
environment
created
during
procedure
By
optimizing
bioink
composition
parameters,
successfully
simulated
vivo
leading
notable
improvements
MSC
characteristics
capacity.
RNA
sequencing
further
confirmed
activation
hypoxia
pathways,
which
are
crucial
for
properties.
These
findings
offer
valuable
insights
leveraging
MSC-based
therapies
regenerative
medicine.
Язык: Английский
The rise of 3D bioprinting: from organs to personalized medicine
Patient-Oriented Medicine and Pharmacy,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 7, 2025
Bioprinting
has
emerged
as
a
groundbreaking
technology
in
the
realms
of
organ
transplantation
and
regenerative
medicine,
addressing
critical
challenges
such
shortages
tissue
repair.
This
review
explores
technological
advancements
innovations
bioprinting,
highlighting
state-of-the-art
techniques,
bioinks,
applications
engineering.
Key
milestones
printing
functional
tissues,
including
vascularized
transplantable
prototypes,
are
discussed
alongside
role
bioprinting
personalized
where
patient-oriented
models
revolutionizing
drug
testing
therapeutic
strategies.
Furthermore,
this
article
examines
ethical
considerations
associated
with
offering
insights
into
its
future
potential
to
transform
global
healthcare.
Язык: Английский
Natural macromolecule-based bioinks for 3D bioprinting: A systematic review of composition, physicochemical characterization, and biomedical applications
Tatiana Muñoz-Castiblanco,
Juan P. Moreno-Marín,
Marlon Osorio
и другие.
Bioprinting,
Год журнала:
2025,
Номер
unknown, С. e00407 - e00407
Опубликована: Март 1, 2025
Язык: Английский
Bioprinting in Organ Transplantation: From Experimental Models to Clinical Prospects
Brazilian Journal of Transplantation,
Год журнала:
2025,
Номер
28(1)
Опубликована: Апрель 25, 2025
Background:
Bioprinting
has
emerged
as
an
innovative
technology
in
organ
transplantation
and
regenerative
medicine,
aiming
to
address
pressing
challenges
such
the
shortage
of
donor
organs
need
for
effective
tissue
repair.
By
leveraging
advanced
3D
printing
techniques,
bioprinting
enables
fabrication
functional
tissues
with
precise
architectural
biological
properties.
Methods:
This
review
provides
in-depth
analysis
latest
advancements
bioprinting,
focusing
on
cutting-edge
development
bioinks,
their
applications
engineering.
It
examines
significant
breakthroughs
creation
vascularized
transplantable
prototypes
explores
role
personalized
medicine.
Results:
The
findings
highlight
transformative
impact
biomedical
field,
particularly
drug
testing,
therapeutic
modeling,
patient-specific
treatment
strategies.
Additionally,
key
challenges—including
technological
limitations,
ethical
concerns,
regulatory
considerations—are
discussed
provide
a
comprehensive
understanding
field’s
progress
potential
obstacles.
Conclusion:
holds
immense
promise
revolutionizing
global
healthcare
by
offering
solutions
shortages
advancing
However,
continued
research
innovation
are
necessary
overcome
existing
facilitate
its
clinical
translation
into
mainstream
medical
practice.
Язык: Английский
Bioimpressão no Transplante de Órgãos: Dos Modelos Experimentais às Perspectivas Clínicas
Brazilian Journal of Transplantation,
Год журнала:
2025,
Номер
28(1)
Опубликована: Апрель 25, 2025
Introdução:
A
bioimpressão
despontou
como
uma
tecnologia
inovadora
no
transplante
de
órgãos
e
na
medicina
regenerativa,
visando
solucionar
desafios
urgentes,
a
escassez
doadores
necessidade
reparo
eficaz
tecidos.
Ao
aproveitar
as
técnicas
avançadas
impressão
3D,
permite
fabricação
tecidos
funcionais
com
propriedades
arquitetônicas
biológicas
precisas.
Métodos:
Esta
revisão
fornece
análise
detalhada
dos
últimos
avanços
em
bioimpressão,
concentrando-se
ponta,
desenvolvimento
biotintas
suas
aplicações
engenharia
Ela
examina
os
significativos
criação
protótipos
vascularizados
transplantáveis
explora
função
da
personalizada.
Resultados:
As
descobertas
destacam
o
impacto
transformador
campo
biomédico,
particularmente
testes
medicamentos,
modelagem
terapêutica
estratégias
tratamento
específicas
para
paciente.
Além
disso,
principais
—
incluindo
limitações
tecnológicas,
preocupações
éticas
considerações
regulatórias
são
discutidos
fornecer
compreensão
abrangente
do
progresso
possíveis
obstáculos.
Conclusão:
é
imensamente
promissora
revolucionar
saúde
global,
oferecendo
soluções
avanço
regenerativa.
No
entanto
pesquisa
inovação
contínuas
necessárias
superar
existentes
facilitar
sua
tradução
clínica
prática
médica
convencional.
Three-dimensional bioprinted gelatin methacryloyl scaffold: a versatile platform for nasal cell culture and drug testing applications
International Journal of Pharmaceutics,
Год журнала:
2025,
Номер
unknown, С. 125803 - 125803
Опубликована: Июнь 1, 2025
Developing
advanced
biomaterials
and
precision
techniques
is
critical
for
tissue
engineering
drug
testing.
This
study
shows
the
design
development
of
a
novel
3D-bioprinted
gelatin
methacryloyl
(GelMA)
scaffold
embedded
with
RPMI-2650
nasal
epithelial
cells
cell
culture
testing
applications.
The
CAD-designed
presented
robust
structure
biocompatibility
across
different
densities.
Studies
swelling
behaviour
displayed
effective
medium
transport
retention,
especially
in
cell-laden
scaffolds.
potential
GelMA
to
enable
3D
growth
was
confirmed
by
continuously
high
viability
(>95
%),
spheroid
formation
substantial
proliferation.
Biocompatibility
live/dead
imaging
coverage
studies,
which
revealed
5-fold
increase
live
over
two
weeks.
These
findings
highlight
scaffolds
as
customisable,
biocompatible,
platform
advancing
screening,
culture,
engineering.
Future
studies
will
focus
on
optimising
composition
incorporating
dynamic
conditions
further
enhance
physiological
relevance
translational
potential.
Язык: Английский