Elucidating the anti-inflammatory potential of nanoscaled polymeric-albumin blends of garcinol: Optimization, in silico, in vitro, and in vivo studies

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 303, С. 140469 - 140469

Опубликована: Фев. 1, 2025

Язык: Английский

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The Role of Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles in Modulating Dermal Fibroblast Activity: A Pathway to Enhanced Tissue Regeneration

Biology, Год журнала: 2025, Номер 14(2), С. 150 - 150

Опубликована: Фев. 1, 2025

Extracellular vesicles (EVs) secreted by umbilical cord-derived mesenchymal stem cells (UC-MSCs) hold significant promise as therapeutic agents in regenerative medicine. This study investigates the effects of UC-MSC-derived EVs on dermal fibroblast function, and their potential wound healing applications. were characterized nanoparticle tracking analysis transmission electron microscopy, revealing a mean size 118.6 nm, consistent with exosomal properties. Dermal fibroblasts treated varying concentrations (25–100 µg/mL), impacts cellular metabolism, mitochondrial activity, reactive oxygen species (ROS) production, closure, inflammatory cytokine secretion, growth factor extracellular matrix (ECM) gene expression evaluated. At lower (25–50 significantly enhanced metabolic activity. However, higher (≥75 µg/mL) increased ROS levels, suggesting hormetic effects. also modulated inflammation reducing pro-inflammatory cytokines (IL-6, TNF-α) while promoting pro-regenerative (IL-33, TGF-β). Treatment 50 µg/mL optimally stimulated closure secretion (VEGF, BDNF, KGF, IGF), upregulated ECM-related (type I III collagen, fibronectin). These findings demonstrate that exert multifaceted including energetics, stimulation cell migration, regulation inflammation, promotion ECM synthesis. highlights novel strategy for tissue regeneration, emphasizing importance optimizing EV concentration maximal efficacy.

Язык: Английский

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Huc-MSCs-derived exosomes alleviate non-alcoholic steatohepatitis by regulating macrophages polarization through miR-24-3p/STING axis

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 21, 2025

There's a scarcity of drugs effective against nonalcoholic steatohepatitis (NASH). Exosomes from Human umbilical cord mesenchymal stem cells (huc-MSCs) show potential in managing glycolipid metabolism and the immune response. Therefore, further investigations are required to explore their application NASH underlying mechanisms. C57BL/6J mice were fed with western diet for 12 weeks induce NASH, huc-MSCs exosomes (MSCs-exo) administered during feeding period. The effect MSCs-exo was evaluated by monitoring changes body weight, fat distribution, blood glucose, insulin levels, analyzing pathological alterations liver tissue. Mechanism carried out using flow cytometry, immunofluorescence staining, other experimental techniques. could reduce fat, inflammation, fibrosis, improved alleviate progression NASH. Besides, decrease macrophage accumulation liver, encouraging M2 over M1 polarization. Furthermore, our study found that had high expression miR-24-3p, which may regulate polarization targeting interferon-stimulated genes (STING) gene macrophages, its overexpression amplifying MSCs-exo's benefits. These findings suggest therapeutic on be attributed regulation through miR-24-3p STING. This provides scientific basis future clinical application.

Язык: Английский

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Click chemistry-based modified exosomes: Towards enhancing precision in cancer theranostics

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160915 - 160915

Опубликована: Фев. 1, 2025

Язык: Английский

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Construction and Osteogenic Capacity Study of Engineered Exosome-based Nanoplatform Targeting Macrophages

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106912 - 106912

Опубликована: Апрель 1, 2025

Язык: Английский

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