Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination

Animals, Год журнала: 2021, Номер 11(4), С. 920 - 920

Опубликована: Март 24, 2021

The aim of the study was to compare morphometric features sperm head size and shape from Pietrain line Duroc × boar crossbred terminal lines, evaluate their relationship with reproductive success after artificial insemination sows produced crossbreeding York, Landrace breeds. Semen samples were collected 11 sexually mature boars. Only ejaculates greater than 70% motility rate <15% abnormal used for inseminations (AI) included in study. Samples analyzed using an ISAS®v1 computer-assisted analysis system eight parameters (CASA-Morph). Sub-populations characterized multivariate procedures, such as principal component (PC) clustering methods (k-means model). Four different ejaculate sub-populations identified two PCs that involved spermatozoa. discriminant ability variables predict sow litter a receiver operating characteristics (ROC) curve analysis. Sperm length, ellipticity, elongation, regularity showed significant predictive capacity on (0.59, 0.59, 0.60, 0.56 area under (AUC), respectively). not related size.

Язык: Английский

---

Fertility after photodynamic inactivation of bacteria in extended boar semen

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Авг. 7, 2024

Antimicrobial resistance is an increasing challenge in semen preservation of breeding animals, especially the porcine species. Bacteria are a natural component semen, and their growth should be inhibited to protect sperm fertilizing capacity female’s health. In pig breeding, where routinely stored at 17°C liquid state, alternatives conventional antibiotics urgently needed. Photodynamic inactivation (PDI) bacteria well-established tool medicine food industry but this technology has not been widely adopted preservation. The specific setting selectively inactivate while maintaining integrity functionality. aim study was test principle PDI boar using photosensitizer 5,10,15,20-tetrakis(N-methyl-4-pyridyl)-21H,23H-porphine (TMPyP) white light LED-setup. first step, photophysical experiments comprising singlet oxygen phosphorescence kinetics TMPyP determination triplet time revealed sufficiently high production reactive Androstar Premium extender, whereas seminal plasma acted as strong quencher. vitro with extended showed that established protocol preserves motility, membrane integrity, DNA mitochondrial activity efficiently reducing below detection limit. A proof-of-concept insemination confirmed vivo fertility after photodynamic treatment. conclusion, approach, innovative controls maintains fertility. This could promising contribution One Health concept potential reduce antimicrobial animal husbandry.

Язык: Английский

---

Analysis of changes in the morphological structures of sperm during preservation of liquid boar semen in two different seasons of the year

Animal Reproduction Science, Год журнала: 2023, Номер 256, С. 107297 - 107297

Опубликована: Июль 14, 2023

Animal fertility is the result of a combination genetic, physiological and environmental factors. Assessment semen quality plays key role in determining reproductive performance boars. The aim study was to determine effect two seasons year on morphology, morphometry, cell membrane integrity mitochondrial activity sperm during storage liquid boar at 17 °C. carried out using 20 Four ejaculates were collected from each year. All tests five times: 1, 24, 48, 96 168 h storage. results showed that summer are more sensitive conditions than those obtained winter. percentage with morphological defects shown increase time diluted ejaculates, particularly between preservation. In summer, an intact high potential much lower winter, every hour case boars used for artificial insemination, it worth taking into account season when collected, especially if be stored form. structures should implemented insemination stations often summer.

Язык: Английский

---

Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods

Animals, Год журнала: 2021, Номер 11(1), С. 227 - 227

Опубликована: Янв. 18, 2021

Evaluation of sperm morphometry is an important criterion in the diagnosis a male animal’s suitability for breeding. The aim study was to evaluate from epididymides dogs subjected routine castration using various staining methods. carried out on semen collected ten healthy dogs. Gonads were obtained each dog during at veterinary surgery. Then, (caput, corpus, cauda) isolated gonads, them and microscope slides prepared. evaluation prepared by four methods: DiffQuik, SpermBlue, eosin-nigrosin eosin-gentian. A total 2400 analyzed (240 dog). caput corpus epididymis found have larger heads tails than those cauda epididymis. method shown affect taken methods differentiate dimensions head different parts but do not length tail. occurrence differences may be linked use fixatives chemical reagents procedure. Sperm stained EN had smallest tail dimensions. greatest area noted EG method. In SB method, relatively long narrow. used are suitable structure, possibility all enables full characterization caput,

Язык: Английский

---

A Bayesian analysis of boar spermatozoa kinematics and head morphometrics and their relationship with litter size fertility variables

Reproduction in Domestic Animals, Год журнала: 2021, Номер 56(7), С. 1024 - 1033

Опубликована: Апрель 29, 2021

The semen movement and sperm head size patterns of boar ejaculates were analysed using computer-assisted analysis (CASA)-Mot -Morph systems. aim the present study was to compare morphometric kinematics variables from boars determine relationship with sow fertility related litter size. females maternal crossing schemes such as continuous 3-generation cross between York (Y), Landrace (L), Pietrain (P) hybrid sows boars. Semen samples collected 11 sexually mature two sire lines. Samples ISAS® v1 system evaluate eight kinematic velocity, progressiveness undulations. Four parameters (length, width, area perimeter) analysed. Bayesian revealed relevant differences in four (VSL, LIN, STR WOB) lines, a probability relevance (PR ) 0.79-0.91, associated higher progressive motility compared Duroc x Moreover, there all = 0.82-0.85) dam line Y-L-50 (½ Y × ½ L) had total born per piglets alive, YLP-75 (1 /8 1 L 3 /4 P) values weight at birth (highest posterior density region 95% 9.92, 16.41 kg). There are assessed lines also relevant. York-Landrace when YLP-50 (¼ ¼ Pietrain). Differences need be further studied.

Язык: Английский

---

Morphometry of Boar Spermatozoa in Semen Stored at 17 °C—The Influence of the Staining Technique

Animals, Год журнала: 2022, Номер 12(15), С. 1888 - 1888

Опубликована: Июль 25, 2022

The aim of the study was to assess morphometry sperm during storage liquid boar semen at 17 °C. An attempt also made evaluate suitability three staining methods for assessment morphometry. carried out on 20 Landrace boars. Semen collected from boars every 5 days by manual method. Four ejaculates each were analysed (80 in total). Analyses performed five times: 1 h, 24 48 96 and 168 h after collection. Blisters with insemination doses opened immediately before analyses. From dose, smears prepared morphometric evaluation sperm, which stained (eosin-nigrosin-EN, eosin-gentian-EG, SpermBlue-SB). Morphometric measurements 15 randomly selected normal morphology slide. included following parameters: head length, width, area, perimeter; tail length; total length. results used calculate shape index. dimensions shown change extent these changes, however, depended method used, as result different case both tail. In slides eosin-nigrosin method, smaller time than SpermBlue eosin-gentian methods.

Язык: Английский

---

Impact of sperm concentration on sperm morphology of large white and landrace boars

Livestock Science, Год журнала: 2020, Номер 241, С. 104214 - 104214

Опубликована: Авг. 14, 2020

Язык: Английский

---

Integrity of Sperm Cell Membrane in the Semen of Crossbred and Purebred Boars during Storage at 17 °C: Heterosis Effects

Animals, Год журнала: 2021, Номер 11(12), С. 3373 - 3373

Опубликована: Ноя. 25, 2021

The aim of the study was to assess changes in integrity sperm cell membranes during storage semen collected from Duroc × Pietrain crossbred boars and purebred component breeds. To compare membrane heads boars, heterosis effects were estimated. conducted on 48 ejaculates used for artificial insemination. Microscope slides prepared each ejaculate evaluation sperm, at 1, 24, 48, 72, 96 h after collection ejaculate. Diluted stored 17 °C. Sperm analysed by two methods: SYBR-14/PI eosin-nigrosin. Our results showed that changed with time, but extent varied depending genetic group boars. crossbreds clearly seen be less sensitive conditions than parent breeds, which confirmed calculated effects. percentage an intact higher (p ≤ 0.05). In addition, significantly fewer moribund spermatozoa a damaged observed should assessed more often This provides valuable information development implementation quality monitoring breeds °C respect structure heads. methods effectively identify damage storage.

Язык: Английский

---

Effect of sperm concentration on boar spermatozoa mitochondrial membrane potential and motility in semen stored at 17 °C

Acta Veterinaria Brno, Год журнала: 2020, Номер 89(4), С. 333 - 340

Опубликована: Янв. 1, 2020

The aim of the study was to assess effect sperm concentration in ejaculate on mitochondrial membrane potential and motility Landrace boar spermatozoa during storage diluted semen at 17 °C. conducted ejaculates collected from 10 boars aged 1.5–2 years. Based measurements, two groups were identified: Group 1 – providing with a least 500 × 3 /mm 2 less than . Four manually each boar. Each Biosolvens Plus diluent, insemination doses prepared stored Mitochondrial evaluated dose. tests carried out after 1, 24, 48, 96 168 h storage. results, it found that ≥ have lower share high below A correlation between demonstrated first 24 storage, which confirmed by calculated phenotypic coefficients. Sperm cells higher are more sensitive time concentration.

Язык: Английский

---

Effects of breed and ejaculate volume on sperm morphology and semen parameters of boars

Animal Science Journal, Год журнала: 2021, Номер 92(1)

Опубликована: Янв. 1, 2021

Abstract The aim of the study was to determine relation between semen quality, frequency sperm defects, dimensions and shape, ejaculate volume Large White Landrace boars. A total 648 ejaculates collected from 31 30 boars were divided into three groups according criterion volume. In this produced with higher volume, concentration, numbers spermatozoa than also showed a lower morphological abnormalities ( P < 0.05). had larger heads, which by 0.15 μm longer, perimeter area boar longer flagellum generally 2.07 Significant differences found in shape two breeds more elongated ratio head size length Sperm low shorter greater length/flagellum medium‐ high‐volume

Язык: Английский

---