Effects of Trehalose Supplementation on Lipid Composition of Rooster Spermatozoa Membranes in a Freeze/Thaw Protocol

Animals, Год журнала: 2023, Номер 13(6), С. 1023 - 1023

Опубликована: Март 10, 2023

The plasma membrane of spermatozoa plays an important role in the formation and maintenance many functions spermatozoa, including during cryopreservation. As a result chromatographic analysis, content lipids fatty acids membranes roosters two breeds was determined under influence cryoprotective media containing trehalose LCM-control (0 mM), Treh20 (9.5 Treh30 (13.4 mM). use diluent made it possible to maintain dynamic balance between synthesis degradation phospholipids sterols frozen/thawed close that native spermatozoa. This contributed increase preservation from 48.3% 52.2% egg breed 30.0% 35.1% meat- and-egg breed. It also noted their kinetic apparatus (mobility indicators) remained at level 45.6% (egg breed) 52.4% (meat-and-egg breed). An concentration 13.4 mM for rooster sperm resulted decrease morphofunctional parameters

Язык: Английский

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The Role of Melatonin to Ameliorate Oxidative Stress in Sperm Cells

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(20), С. 15056 - 15056

Опубликована: Окт. 11, 2023

It is widely accepted that oxidative stress (OS) coming from a wide variety of causes has detrimental effects on male fertility. Antioxidants could have significant role in the treatment infertility, and current systematic review melatonin to ameliorate OS clearly shows improvement semen parameters follows supplementation. Although considerable promise, further studies are needed clarify its ability preserve or restore quality under conditions varied species. The present examines actions via receptor subtypes function context across vertebrates.

Язык: Английский

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Reproductive physiology of the boar: What defines the potential fertility of an ejaculate?

Animal Reproduction Science, Год журнала: 2024, Номер 269, С. 107476 - 107476

Опубликована: Апрель 18, 2024

Despite decades of research and handling semen for use in artificial insemination (AI) other assisted reproductive technologies, 5-10% selected boar sires are still considered sub-fertile, escaping current assessment methods sperm quality resilience to preservation. As end-product, the ejaculate (emitted spermatozoa sequentially exposed composite seminal plasma, SP) ought define homeostasis testes, epididymis, accessory sexual glands. Yet, linking findings production biology fertility is suboptimal. The present essay critically reviews how a fertile can help us diagnose both health handling, focusing on -available under development- identify suitable biomarkers cryotolerance fertility. Bulk SP, proteins microRNAs (miRNAs) have, albeit linked function after AI, failed enhance outcomes at commercial level, perhaps just being components complex functional pathway. Hence, focus now interaction sperm-SP, comparing vivo with ex vivo, regarding nano-sized lipid bilayer extracellular vesicles (sEVs) as priority. sEVs transport fragile molecules (lipids, proteins, nucleic acids) which, shielded from degradation, mediate cell-to-cell communication female internal genital tract. Such modulates essential processes, immunological tolerance. be harvested, characterized, stored, manipulated, e.g. used andrological diagnosis, selection breeders, alternatively additives improve cryosurvival

Язык: Английский

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Reduction of Prostate Cancer Risk: Role of Frequent Ejaculation-Associated Mechanisms

Cancers, Год журнала: 2025, Номер 17(5), С. 843 - 843

Опубликована: Фев. 28, 2025

Prostate cancer (PCa) accounts for roughly 15% of diagnosed cancers among men, with disease incidence increasing worldwide. Age, family history and ethnicity, diet, physical activity, chemoprevention all play a role in reducing PCa risk. The prostate is an exocrine gland that characterized by its multi-functionality, being involved reproductive aspects such as male ejaculation orgasmic ecstasy, well playing key roles the regulation local systemic concentrations 5α-dihydrotestosterone. increase androgen receptors at ventral first elevated response induced copulation. growth function mediated androgen-dependent mechanism. Binding 5-DHT to (AR) results formation 5α-DHT:AR complex. interaction complex specific DNA enhancer element androgen-regulated genes leads androgen-specific target maintain homeostasis. Consequently, may significant reduction Thus, frequent absence risky sexual behavior possible approach prevention PCa. In this review, we provide insight into mechanisms regulating impact on

Язык: Английский

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The Nexus Between Sperm Membrane Integrity, Sperm Motility, and DNA Fragmentation

Membranes, Год журнала: 2025, Номер 15(4), С. 109 - 109

Опубликована: Апрель 2, 2025

This study investigated the interrelationships between sperm plasma membrane integrity, motility, and DNA fragmentation (SDF) to provide a more holistic understanding of male fertility. A total 1159 ejaculates were analyzed for integrity (% dead spermatozoa), motility immotile SDF with fragmented DNA). The statistical methods included non-parametric correlation analysis artificial intelligence (AI)-generated cluster identify patterns based on these three parameters. results showed moderate (ρ = 0.65; p < 0.000) indicating that likely exhibit damage. weak 0.21; suggested damage was largely independent other Cluster identified main clusters: 0: high levels low sperm, fragmentation. 1: but extremely 2: lowest damage, fragmentation, overall better quality. clustering techniques demonstrated their ability integrate multiple parameters, enabling individualized fertility diagnosis potentially enhancing infertility assessments.

Язык: Английский

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Effect of Bovine Serum Albumin (BSA) Concentration on Cryopreservation of Booroolong Frog Sperm with Evaluation of Post-Thaw Motility in Caffeine

Veterinary Sciences, Год журнала: 2025, Номер 12(1), С. 30 - 30

Опубликована: Янв. 8, 2025

Reproductive technologies, including sperm cryopreservation, offer conservationists enhanced capacity to genetically manage populations and improve the outcomes of conservation breeding programs (CBPs). Despite this potential, post-thaw quality amphibian is highly variable following research focused on protocol refinement needed. The aim study was twofold: (1) investigate effect addition bovine serum albumin (BSA) cryopreservation medium (pre-freeze), (2) caffeine activation (post-thaw), characteristics in critically endangered Booroolong frog (Litoria booroolongensis). Spermic urine samples were collected from 14 male frogs hormonal induction spermiation, each sample split among three treatments, where contained either 0 (control), 0.5, or 1% BSA (w/v). Samples cryopreserved thawed, motility then activated one two treatments: Milli-Q water (control) plus 4.5 mM caffeine. Sperm viability (proportion live/dead) assessed using fluorescent microscopy, metrics evaluated computer-assisted analysis (CASA). Results showed that concentration had no viability. Additionally, neither nor influenced (total motility, forward progressive velocity). Assessment time varied 5 13 min post-activation significantly correlated with measure, velocity decreasing as increased. results reported herein provide evidence for an at concentrations tested frog, but they highlight time-sensitive nature assessment implications timing handling during assisted fertilisation efforts.

Язык: Английский

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Impact of leachate from boiled-water-treated plastic products on male reproductive health: Insights from transcriptomic and metabolomic profiling

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 290, С. 117748 - 117748

Опубликована: Янв. 1, 2025

Given the extensive use of plastic materials in modern society, there is an escalating concern about potential risks associated with exposure to products. This study investigated impact leachates from boiled-water-treated cups, including polypropylene (PP), high-density polyethylene (HDPE), low-density (LDPE), polystyrene (PS), and terephthalate (PET), on male reproductive health. Experimental mice were administered daily doses for 180 consecutive days. Histological analysis testes epididymis was conducted, revealing vacuolization absence sperms seminiferous tubules treated PP, HDPE, LDPE, PS, while PET exhibited lower toxicity. Furthermore, transcriptomic metabolomic profiling employed identify key genes metabolites related exposure. Transcriptome showed significant changes spermatogenesis following leachates, metabolome indicated lipid metabolism pathway. Overall, provides evidence that oral cups could negatively affect metabolism, thereby posing These findings offer crucial insights into consumption may advocate selection relatively safe everyday use.

Язык: Английский

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Stored and Frozen Semen in Swine

Опубликована: Янв. 1, 2025

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Redox-Driven Epigenetic Modifications in Sperm: Unraveling Paternal Influences on Embryo Development and Transgenerational Health

Antioxidants, Год журнала: 2025, Номер 14(5), С. 570 - 570

Опубликована: Май 9, 2025

Male-factor infertility accounts for nearly half of all cases, and mounting evidence points to oxidative stress as a pivotal driver sperm dysfunction, genetic instability, epigenetic dysregulation. In particular, the DNA lesion 8-hydroxy-2′-deoxyguanosine (8-OHdG) has emerged central mediator at interface damage regulation. We discuss how this can disrupt key mechanisms such methylation, histone modifications, small non-coding RNAs, thereby influencing fertilization outcomes, embryo development, offspring health. propose that interplay between reprogramming is further exacerbated by aging in both paternal maternal germlines, creating “perfect storm” increases risk heritable (epi)mutations. The consequences unresolved lesions thus persist beyond fertilization, contributing transgenerational health risks. Finally, we explore promise potential pitfalls antioxidant therapy strategy mitigate damage. While supplementation may hold significant therapeutic value men with subfertility experiencing elevated stress, careful, personalized approach essential avoid reductive unintended disruptions. Recognizing dual role shaping genome epigenome underscores need integrating redox biology into reproductive medicine, aim improving fertility treatments safeguarding future generations.

Язык: Английский

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Improvement of post-thaw quality and fertilizing ability of bull spermatozoa using Rho kinase inhibitor in freezing extender

Frontiers in Veterinary Science, Год журнала: 2023, Номер 10

Опубликована: Июль 6, 2023

In this study, it was hypothesized that the addition of an appropriate concentration Y-27632 (a ROCK inhibitor) to freezing extender prevents cryopreservation-induced apoptosis and improves embryonic development after in vitro fertilization (IVF). Semen samples were collected from five fertile Simmental bulls using artificial vagina twice a week for 4 weeks. Selected pooled diluted with Tris-egg-yolk-glycerol (TEYG) containing different concentrations (0, 10, 20, 30, 40 μM) then frozen liquid nitrogen. After thawing, computer-assisted semen analysis (CASA), plasma membrane integrity, acrosome intactness evaluated terms morphological abnormalities, intracellular generation reactive oxygen species (ROS), DNA fragmentation, phosphatidylserine (PS) externalization, apoptotic-related gene expression. Finally, groups thawed spermatozoa used bovine oocyte IVF. The results show at 20 μM effectively improved total motility (TM), curvilinear velocity (VCL), as well integrity compared control group ( p &lt; 0.05). Intracellular ROS levels significantly 0.05) lower treated 30 specimen. Furthermore, supplementation resulted more viable According qRT-PCR results, expression BAX CASPASE-9 genes downregulated, while BCL2 increased IVF demonstrated treatment frozen–thawed blastocyst rates conclusion, into can improve functionality fertilizing capacity due its antioxidative anti-apoptotic properties.

Язык: Английский

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