Cryopreservation of rabbit semen: impacts of permeable and non-permeable mixture of cryoprotectant, male group individuality, freezing rate, semen package size and antioxidant bovine serum albumin on rabbit semen freezability DOI Creative Commons

Kamel M. Mohammed,

Gamal M. Darwish,

Zaher M. Rawash

и другие.

World Rabbit Science, Год журнала: 2022, Номер 30(3), С. 227 - 238

Опубликована: Сен. 30, 2022

In the present study, three experiments were designed to identify most appropriate technique for freezing rabbit semen. Experiment 1 aimed determine optimal levels of dimethyl sulfoxide (DMSO) contents in medium and their effects on individual bucks. Semen ejaculates each buck (n=15 bucks) mixed split into portions extension with a containing varying concentrations DMSO (0.75, 1.0, 1.4 M). Diluted semen samples packaged 0.25 mL straws suspended above liquid nitrogen (LN) 10 min, then dipped LN. A few days after freezing, post-thaw evaluation was assessed, according results, six bucks an extender 0.75 M used 2 3. experiment 2, pooled from 6 divided two packaging straw sizes (0.25 0.50 mL). Each size five groups at different heights LN (2, 4, 6, 8, cm) minutes before being preserved 3, four dilution bovine serum albumin (BSA; 0, 2.5, 5.0, 7.5 mg/mL). 4 cm freezing. Pre-freezing post-thawing, evaluated quality. Results showed that had higher significant values sperm motility, longevity, acrosome integrity plasma membrane permeability. Bucks’ individuality integrity. interaction recorded between bucks’ longevity. package no parameters. frozen significantly better BSA 5 mg/mL improved recovery rates seem be more adequate cryopreservation. The level differs bucks, as longevity is affected. enhanced obtained will need further investigation confirmed field.

Язык: Английский

Canine Spermatozoa—Predictability of Cryotolerance DOI Creative Commons
Sabine Schäfer‐Somi, Martina Colombo, G.C. Luvoni

и другие.

Animals, Год журнала: 2022, Номер 12(6), С. 733 - 733

Опубликована: Март 15, 2022

Markers of freezability allow the selection ejaculates good freezability. So far, most investigations were conducted in boars, bulls, rams and horses, with high economic interests triggering efforts. The progress dogs is comparably slow. A critical evaluation methods requires consideration practicability, labs not even possessing a computer assisted sperm analyser (CASA); furthermore, small canine mostly do use large semen volumes. In dogs, modern markers no longer assess single membrane constituents or seminal plasma components but comprise tests cell functionality adaptability, energy metabolism, cluster analyses kinetic morphometric parameters, as well DNA intactness. Identification efficient combination seems useful. At present, examination by CASA combined analysis subgroups, JC-1 staining COMET assay toluidine blue seem appropriate; however, volumetry other functional deserve better attention. understanding spermatozoa metabolism might reveal new markers. This review focuses on requirements semen, highlighting potential future candidates.

Язык: Английский

Процитировано

6
Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation DOI Creative Commons
Bianca Barreto Barbosa, Inara Tayná Alves Evangelista, Airton Renan Bastos Soares

и другие.

Animal Reproduction, Год журнала: 2023, Номер 20(2)

Опубликована: Янв. 1, 2023

Sperm cryopreservation is an important tool for genetic diversity management programs and the conservation of endangered breeds species. The most widely used method sperm slow freezing, however, during process, cells suffer from cryoinjury, which reduces their viability fertility rates. One alternatives to freezing vitrification, that consist on rapid in viable undergo glass-like solidification. This technology requires large concentrations permeable cryoprotectants (P- CPA’s) increase viscosity medium prevent intracellular ice formation cooling warming, obtaining successful results vitrification oocytes embryos. Unfortunately, this failed when applied due its higher sensitivity increasing P-CPAs. Alternatively, a technique termed ‘kinetic vitrification’ has been consists permeant cryoprotectant-free by direct plunging suspension into liquid nitrogen. Some advantages kinetic are speed execution no rate-controlled equipment required. successfully with better motility human (50-70% recovery), dog (42%), fish (82%) donkey (21.7%). However, more studies required improve after devitrification, especially it comes recovery. objective review present principles main findings literature, perspectives utilization as method.

Язык: Английский

Процитировано

3
Ether lipids and a peroxisomal riddle in sperm DOI Creative Commons
Mayrene Horta Remedios,

Weisheng Liang,

Lucas N González

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Май 15, 2023

Sperm are terminally differentiated cells that lack most of the membranous organelles, resulting in a high abundance ether glycerolipids found across different species. Ether lipids include plasmalogens, platelet activating factor, GPI-anchors and seminolipid. These play important roles sperm function performance, thus special interest as potential fertility markers therapeutic targets. In present article, we first review existing knowledge on relevance types for production, maturation function. To further understand ether-lipid metabolism sperm, then query available proteomic data from highly purified produce map metabolic steps retained these cells. Our analysis pinpoints presence truncated lipid biosynthetic pathway would be competent production precursors through initial peroxisomal core steps, but devoid subsequent microsomal enzymes responsible final synthesis all complex ether-lipids. Despite widely accepted notion peroxisomes, thorough published conducted herein identifies nearly 70% known resident proteins part proteome. view this, highlight open questions related to possible functions sperm. We propose repurposed role detoxification products oxidative stress, which is critically influence The likely peroxisomal-derived remnant compartment could act sink toxic fatty alcohols aldehydes generated by mitochondrial activity discussed. With this perspective, our provides comprehensive associated with ether-lipids peroxisomal-related offers new insights into potentially relevant antioxidant mechanisms warrant research.

Язык: Английский

Процитировано

3
Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days DOI
Amr S. El‐Shalofy, Camille Gautier, Younis Mohd Khan

и другие.

Animal Reproduction Science, Год журнала: 2023, Номер 256, С. 107307 - 107307

Опубликована: Июль 23, 2023

Язык: Английский

Процитировано

3
Cryopreservation of rabbit semen: impacts of permeable and non-permeable mixture of cryoprotectant, male group individuality, freezing rate, semen package size and antioxidant bovine serum albumin on rabbit semen freezability DOI Creative Commons

Kamel M. Mohammed,

Gamal M. Darwish,

Zaher M. Rawash

и другие.

World Rabbit Science, Год журнала: 2022, Номер 30(3), С. 227 - 238

Опубликована: Сен. 30, 2022

In the present study, three experiments were designed to identify most appropriate technique for freezing rabbit semen. Experiment 1 aimed determine optimal levels of dimethyl sulfoxide (DMSO) contents in medium and their effects on individual bucks. Semen ejaculates each buck (n=15 bucks) mixed split into portions extension with a containing varying concentrations DMSO (0.75, 1.0, 1.4 M). Diluted semen samples packaged 0.25 mL straws suspended above liquid nitrogen (LN) 10 min, then dipped LN. A few days after freezing, post-thaw evaluation was assessed, according results, six bucks an extender 0.75 M used 2 3. experiment 2, pooled from 6 divided two packaging straw sizes (0.25 0.50 mL). Each size five groups at different heights LN (2, 4, 6, 8, cm) minutes before being preserved 3, four dilution bovine serum albumin (BSA; 0, 2.5, 5.0, 7.5 mg/mL). 4 cm freezing. Pre-freezing post-thawing, evaluated quality. Results showed that had higher significant values sperm motility, longevity, acrosome integrity plasma membrane permeability. Bucks’ individuality integrity. interaction recorded between bucks’ longevity. package no parameters. frozen significantly better BSA 5 mg/mL improved recovery rates seem be more adequate cryopreservation. The level differs bucks, as longevity is affected. enhanced obtained will need further investigation confirmed field.

Язык: Английский

Процитировано

4