Macedonian Veterinary Review,
Год журнала:
2023,
Номер
46(2), С. 177 - 183
Опубликована: Сен. 28, 2023
Abstract
Semen
cooling
and
cryopreservation
have
revolutionized
the
field
of
reproductive
biotechnologies.
However,
challenges
persist
in
maintaining
sperm
quality
viability
during
these
processes.
The
unsatisfactory
results
main
problems
are
associated
with
low
quality,
viability,
morphological,
structural,
DNA
integrity,
changes
plasma
membrane,
ability
to
interact
female
tract
decrease
fertilization
potential.
objective
this
study
was
determine
effect
seminal
proteins
different
molecular
weights
on
kinetic
parameters
dog
spermatozoa
cold
storage
at
4
°C.
were
isolated
from
dogs’
ranging
between
6-200
kDa,
separated
four
fractions.
ejaculates
(n=15)
collected
6
heathy
dogs
which
pooled.
Spermatozoa
extended
Tris
medium
alone
(control)
or
addition
one
protein
fractions,
then
incubated
°C
for
2
hours.
Sperm
10-15
kDa
had
significantly
higher
total
motility
(37.01±2.98%),
progressiveness
(15.97±1.91%),
curvilinear
line
velocity
(37.46±3.75
μm/s),
linearity
(26.18±1.00%),
straightness
(45.94±2.03%)
compared
other
groups
weight
control
group.
findings
indicated
that
use
certain
can
be
beneficial
reducing
detrimental
preserving
spermatozoa.
It
seemed
presence
canine
rendered
less
amenable
negative
influence
cooling.
Animal Reproduction Science,
Год журнала:
2024,
Номер
269, С. 107476 - 107476
Опубликована: Апрель 18, 2024
Despite
decades
of
research
and
handling
semen
for
use
in
artificial
insemination
(AI)
other
assisted
reproductive
technologies,
5-10%
selected
boar
sires
are
still
considered
sub-fertile,
escaping
current
assessment
methods
sperm
quality
resilience
to
preservation.
As
end-product,
the
ejaculate
(emitted
spermatozoa
sequentially
exposed
composite
seminal
plasma,
SP)
ought
define
homeostasis
testes,
epididymis,
accessory
sexual
glands.
Yet,
linking
findings
production
biology
fertility
is
suboptimal.
The
present
essay
critically
reviews
how
a
fertile
can
help
us
diagnose
both
health
handling,
focusing
on
-available
under
development-
identify
suitable
biomarkers
cryotolerance
fertility.
Bulk
SP,
proteins
microRNAs
(miRNAs)
have,
albeit
linked
function
after
AI,
failed
enhance
outcomes
at
commercial
level,
perhaps
just
being
components
complex
functional
pathway.
Hence,
focus
now
interaction
sperm-SP,
comparing
vivo
with
ex
vivo,
regarding
nano-sized
lipid
bilayer
extracellular
vesicles
(sEVs)
as
priority.
sEVs
transport
fragile
molecules
(lipids,
proteins,
nucleic
acids)
which,
shielded
from
degradation,
mediate
cell-to-cell
communication
female
internal
genital
tract.
Such
modulates
essential
processes,
immunological
tolerance.
be
harvested,
characterized,
stored,
manipulated,
e.g.
used
andrological
diagnosis,
selection
breeders,
alternatively
additives
improve
cryosurvival
Animal Reproduction Science,
Год журнала:
2022,
Номер
244, С. 107051 - 107051
Опубликована: Авг. 3, 2022
Seminal
plasma
(SP)
is
not
a
pre-requisite
for
pregnancy.
Yet,
this
heterogeneous,
composite
SP
has
proven
relevant
fertility,
as
mediator
cell-to-cell
communication
between
producing
cells,
spermatozoa
and
the
female
internal
genital
tract,
regulating
complex
reproductive
processes.
Bearing
hormones,
proteins,
cytokines
well
nuclei
acids
in
nano-sized
lipid
bilayer
seminal
extracellular
vesicles
(sEVs),
concerts
signaling
to
female.
Signals
influence
timing
of
ovulation,
sperm
transport
and,
particularly,
enable
immune
system
balance
her
cryptic
choice
engage
into
pregnancy
or
reject
an
eventual
fertilization.
This
essay,
focusing
on
livestock
general
pigs
particular,
discusses
intrinsic
roles
sEVs
with
regards
homeostasis,
while
binding
internalizing
their
cargo
tract
epithelia
regulate
functional
activity.
Since
prior
studies
had
inconclusive
results
using
bulk
single
SP-contained
free
molecules,
argumentation
hereby
provided
increase
current
incipient
research
sEVs,
where
fragile
molecules
fertility
are
shielded
from
degradation
during
handling.
EVs
isolated
can
be
used
andrological
diagnosis
perhaps
select
breeders
optimal
fertility.
Moreover,
laboratory-uploaded
specific
even
engineered
nanodroplets
additives
extenders
improve
after
artificial
insemination
(AI)
biotechnologies.
Animal Production Science,
Год журнала:
2024,
Номер
64(9)
Опубликована: Июнь 13, 2024
Context
Collecting,
cooling,
and
cryopreserving
semen
is
essential
for
accessing
genetically
superior
stallions.
However,
preserving
stallion
sperm
presents
unique
challenges
compared
with
other
species.
Aims
This
study
aimed
to
investigate
the
effect
of
zinc
(Zn)
supplementation
on
quality
equine
during
cold
storage.
Methods
Various
factors
contributing
were
assessed
at
24
48
h
after
subjecting
samples
a
heat-resistance
test.
In
Experiment
1,
four
experimental
groups
examined,
each
different
concentrations
Zn
sulfate,
as
follows:
0,
2,
3
mM.
Subsequently,
2
involved
testing
wider
range
(0,
0.1,
0.2,
0.4,
0.8,
1.6,
3.2
mM),
including
evaluation
incubation
240
min
37°C
(heat-resistance
test).
Key
results
The
addition
extender
INRA96
did
not
yield
substantial
improvements
in
sperm-quality
parameters
cooling
or
h.
Moreover,
no
protective
benefits
observed
when
underwent
Concentrations
surpassing
mM
had
an
adverse
parameters.
Conclusions
implications
These
findings
have
contributed
understanding
strategy
improving
preservation
Biopreservation and Biobanking,
Год журнала:
2022,
Номер
21(4), С. 336 - 345
Опубликована: Июль 20, 2022
The
present
study
was
conducted
to
observe
the
effects
of
removal
seminal
plasma
Pantja
buck
semen
and
supplementation
bovine
(BSP)
in
extender
before
cryopreservation.
In
a
preliminary
experiment,
different
levels
BSP
were
supplemented
(1,
3,
5,
7,
9%
v/v)
egg
yolk
(7.5%
yolk)-tris
(EYT)
used
for
cryopreservation
semen.
Results
terms
motility,
viability,
membrane
integrity,
acrosome
lipid
peroxidation
showed
that
5%
suitable
maintaining
quality
during
final
pooled
from
four
bucks
split
into
three
aliquots
(I,
II,
III).
Aliquot
I
directly
diluted
EYT
grouped
as
control
(C);
aliquot
II
III
washed
separately
with
TALP
solution
D1
(Washed
extender)
D2
containing
BSP),
respectively.
Seminal
attributes
(sperm
individual
total
morphological
abnormalities)
assessed
at
postdilution,
postequilibration,
post-thawing
stages.
Alanine
aminotransferase
(ALT),
aspartate
(AST),
malondialdehyde
(MDA)
concentration,
glutathione
peroxidase
(GSH-Px)
activity
measured
post-thaw.
Washed
significantly
improved
(p
<
0.05)
parameters
post-thaw
compared
unwashed
(control).
A
significant
difference
observed
between
freezing
stages
dilution
groups.
Significantly
higher
sperm
GSH-Px
activity,
lower
MDA
concentration
extracellular
release
enzymes
(ALT,
AST)
group
D1.
results
demonstrated
BSP-supplemented
can
improve
quality.
