Macedonian Veterinary Review,
Год журнала:
2023,
Номер
46(2), С. 177 - 183
Опубликована: Сен. 28, 2023
Abstract
Semen
cooling
and
cryopreservation
have
revolutionized
the
field
of
reproductive
biotechnologies.
However,
challenges
persist
in
maintaining
sperm
quality
viability
during
these
processes.
The
unsatisfactory
results
main
problems
are
associated
with
low
quality,
viability,
morphological,
structural,
DNA
integrity,
changes
plasma
membrane,
ability
to
interact
female
tract
decrease
fertilization
potential.
objective
this
study
was
determine
effect
seminal
proteins
different
molecular
weights
on
kinetic
parameters
dog
spermatozoa
cold
storage
at
4
°C.
were
isolated
from
dogs’
ranging
between
6-200
kDa,
separated
four
fractions.
ejaculates
(n=15)
collected
6
heathy
dogs
which
pooled.
Spermatozoa
extended
Tris
medium
alone
(control)
or
addition
one
protein
fractions,
then
incubated
°C
for
2
hours.
Sperm
10-15
kDa
had
significantly
higher
total
motility
(37.01±2.98%),
progressiveness
(15.97±1.91%),
curvilinear
line
velocity
(37.46±3.75
μm/s),
linearity
(26.18±1.00%),
straightness
(45.94±2.03%)
compared
other
groups
weight
control
group.
findings
indicated
that
use
certain
can
be
beneficial
reducing
detrimental
preserving
spermatozoa.
It
seemed
presence
canine
rendered
less
amenable
negative
influence
cooling.
Advances in Animal and Veterinary Sciences,
Год журнала:
2023,
Номер
11(7)
Опубликована: Янв. 1, 2023
The
reproductive
performance
of
dairy
cows
in
Indonesia
still
needs
to
be
improved.The
method
Double-dose
AI
can
highly
improve
the
success
AI.The
materials
used
were
160
divided
into
80
inseminated
by
a
single
dose
and
with
double
dose.There
two
treatments
this
research;
T1
was
at
8th
hour
after
estrus
onset,
while
T2
2nd
onset.The
Non-Return
Rate
(NRR
1)
observed
on
next
cycle
AI.If
showed
sign,
insemination
repeated
same
treatment
then
continued
observe
NRR
2
cycle.After
months,
pregnancy
failure
using
ultrasound.A
Chi
Square
performed
SAS
OnDemand
for
Academics
(ODA,
Cary,
NC,
USA).Moreover,
probability
values
calculated
least
significant
different
testing.The
results
that
NRR-1
83.75%
85%,
NRR-2
62.5%
68.75%
T2,
respectively.Furthermore,
mainly
caused
repeat
breeders,
which
28.75%
T2.It
consists
95.65%
87.5%
normal
ovaries,
Corpus
luteum
persistent
4.35%
8.34%,
ovarian
hypo
function
0%
4.16%
respectively.In
conclusion,
Artificial
Insemination
doses
did
not
significantly
rate
cows.
American Journal of Animal and Veterinary Sciences,
Год журнала:
2024,
Номер
19(3), С. 200 - 209
Опубликована: Март 1, 2024
The
study
aims
to
compare
different
concentrations
(0,
2.5,
5,
7.5
and
10
mM)
of
antioxidants,
Dithiothreitol
(DTT)
Glutathione
(GSH),
on
sperm
parameters
following
the
freeze-thawing
semen
from
large
white
boars.
Three
boars
were
selected
for
collection
collected
was
sent
lab
analysis.
Following
dilution
with
Beltsville
thawing
solution
120
min
equilibration
at
17°C,
centrifuged
800
g
15°C.
supplementation
DTT
or
GSH
doses
mM),
transferred
into
0.25
mL
freezing
straws.
After
20
in
Liquid
Nitrogen
(LN2)
vapor,
straws
submerged
LN2
tank,
which
a
temperature
-196°C.
37°C,
assessed
several
characteristics.
Data
analyzed
using
GenStat®
statistical
program
generalized
linear
model
procedure.
Treatment
means
separated
Fisher's
protected
t-test
least
significant
difference
0.05
level
significance
(Mean
±
SD).
When
compared
all
treatments,
proportion
total
motility
progressive
higher
control
(51.1±12.7;
27.1±10.8),
5
mM
(45.2±16.1;
24.7±11.3)
(43.8±10.3;
23.9±10.7)
(p<0.05).
rapid
highest
(21.3%)
(21.5%)
treatments
(46.8±6.5),
(44.8±8.2)
(40.3±2.20)
had
high
percentage
normal
live
as
In
hypo-osmotic
swelling
test,
swollen
tail
greater
treatment
(38.8±6.1)
than
(33.6±4.9)
(32.8±4.5)
However,
differences
not
statistically
significant.
conclusion,
quantities
added
extenders
appropriate
cryopreserving
boar
semen.
BIO Web of Conferences,
Год журнала:
2024,
Номер
149, С. 01002 - 01002
Опубликована: Янв. 1, 2024
The
dependence
of
the
results
on
individual
qualities
stallion
semen
is
very
high,
which
an
obstacle
to
wider
use
cryopreservation
method
in
practice.
It
has
been
shown
this
work
that
from
successive
samples
same
different
hydrogen
ion
concentrations
(pH)
and
buffer
capacities.
When
Lactose-chelate-citrate-yalk
environment
(LCHCY)
diluted,
pH
varies
depending
its
initial
value.
But
30-40%
cases,
concentration
shifts
more
or
less
expected
In
order
establish
maximum
possible
survival
time
refrigerated
cryopreserved
semen,
optimum
acidity
value
after
dilution
was
determined.
depended
sperm
concentration.
adjust
acidic
by
dropwise
addition
solutions
medium
alkaline
side.
An
approach
procedure
adjusting
makes
it
increase
number
stallions
for
artificial
insemination
with
resistant
refrigeration
cryopreservation.
Macedonian Veterinary Review,
Год журнала:
2023,
Номер
46(2), С. 177 - 183
Опубликована: Сен. 28, 2023
Abstract
Semen
cooling
and
cryopreservation
have
revolutionized
the
field
of
reproductive
biotechnologies.
However,
challenges
persist
in
maintaining
sperm
quality
viability
during
these
processes.
The
unsatisfactory
results
main
problems
are
associated
with
low
quality,
viability,
morphological,
structural,
DNA
integrity,
changes
plasma
membrane,
ability
to
interact
female
tract
decrease
fertilization
potential.
objective
this
study
was
determine
effect
seminal
proteins
different
molecular
weights
on
kinetic
parameters
dog
spermatozoa
cold
storage
at
4
°C.
were
isolated
from
dogs’
ranging
between
6-200
kDa,
separated
four
fractions.
ejaculates
(n=15)
collected
6
heathy
dogs
which
pooled.
Spermatozoa
extended
Tris
medium
alone
(control)
or
addition
one
protein
fractions,
then
incubated
°C
for
2
hours.
Sperm
10-15
kDa
had
significantly
higher
total
motility
(37.01±2.98%),
progressiveness
(15.97±1.91%),
curvilinear
line
velocity
(37.46±3.75
μm/s),
linearity
(26.18±1.00%),
straightness
(45.94±2.03%)
compared
other
groups
weight
control
group.
findings
indicated
that
use
certain
can
be
beneficial
reducing
detrimental
preserving
spermatozoa.
It
seemed
presence
canine
rendered
less
amenable
negative
influence
cooling.
