Screening of key genes involved in endometritis in cows and the regulatory role of CD83 in bovine endometrial epithelial cells
International Immunopharmacology,
Год журнала:
2025,
Номер
148, С. 114183 - 114183
Опубликована: Янв. 31, 2025
Язык: Английский
HADHA promotes apoptosis and inflammatory response in bovine endometrial epithelial cells by regulating transcription and metabolism
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 140980 - 140980
Опубликована: Фев. 1, 2025
Язык: Английский
Identification of candidate blood biomarkers through metabolomics analysis in bovine superovulation
Frontiers in Veterinary Science,
Год журнала:
2025,
Номер
12
Опубликована: Апрель 30, 2025
Superovulation
and
embryo
transfer
technologies
provide
strong
support
for
improving
the
productivity
of
cattle
population.
A
non-invasive
diagnostic
method
superovulation
prediction
is
necessary
to
improve
its
efficiency.
Compared
macromolecular
substances,
there
has
been
an
increasing
number
studies
on
small
molecular
metabolites
as
biomarkers.
This
study
aimed
identify
key
biomarkers
associated
with
outcomes
in
cows
through
serum
metabolomics
analysis.
In
this
study,
36
induced
estrus
were
selected,
blood
samples
collected
at
three
time
points:
before
FSH
injection,
artificial
insemination,
collection.
Then,
classified
into
high
embryonic
yield
(HEY)
low
(LEY)
groups
based
total
embryos.
Furthermore,
a
untargeted
analysis
two
was
conducted
using
liquid
chromatography
tandem
mass
spectrometry
(LC-MS/MS).
372
embryos
collected.
The
revealed
that
1,158
detected,
617
annotated.
injection
samples,
121
differential
identified
between
groups.
insemination
129
identified.
collection
201
11
shared
while
five
across
all
samples.
majority
significantly
enriched
pathways
related
amino
acid
fatty
metabolism,
digestive
system
secretion,
ovarian
steroidogenesis.
showed
phosphatidylcholine
[PC;
14:0/22:1(13Z)],
phosphatidylethanolamine
[PE;
DiMe
(11,
3)],
triacylglycerol
[TG;
15:0/16:0/22:4
(7Z,
10Z,
13Z,
16Z)],
phosphatidylinositol
[PI;
16:0/22:2
(13Z,
phosphatidylserine
[PS;
18:0/20:4(8Z,
11Z,
14Z,
17Z)]
differentially
expressed
during
period.
These
could
serve
potential
bovine
superovulation.
lipid
metabolic
may
have
impact
response.
results
novel
screening
indexes
donors
superovulation,
although
accuracy
relevant
factors
requires
further
investigation.
Язык: Английский
Morinda officinalis Oligosaccharides Protect Against LPS-Induced Uterine Damage and Endometrial Inflammation in Mice and Bovine Endometrial Epithelial Cells
Animals,
Год журнала:
2025,
Номер
15(9), С. 1286 - 1286
Опубликована: Апрель 30, 2025
Endometritis
poses
a
significant
challenge
to
the
dairy
industry,
impairing
bovine
reproductive
performance
and
causing
substantial
economic
losses.
Although
Morinda
officinalis
oligosaccharides
(MOO)
exhibit
anti-inflammatory
properties,
their
therapeutic
potential
against
endometritis
remains
unclear.
This
study
investigated
MOO's
protective
effects
LPS-induced
uterine
injury
in
mice
inflammation
endometrial
epithelial
cells
(BENDs),
explored
underlying
mechanisms.
In
mice,
MOO
attenuated
by
improving
histopathology,
reducing
pro-inflammatory
cytokines
decreasing
oxidative
stress.
BEND
cells,
alleviated
inflammation,
stress,
apoptosis
via
downregulating
mediators
(IL-1β,
IL-6,
TNF-α,
IL-8,
TLR4,
RELA),
restoring
antioxidant
enzymes
(HMOX1,
NQO1,
Nrf2,
NOX4),
modulating
markers
(BAX,
cleaved
CASP3,
CASP9,
BCL2).
reduced
ROS
accumulation,
preserved
mitochondrial
membrane
potential,
inhibited
calcium
influx.
Critically,
channel
agonist
Bay
K
8644
reversed
effects,
confirming
signaling
modulation
as
key
mechanism.
provides
first
evidence
that
mitigates
damage
BENDs
through
regulation,
suggesting
its
for
treating
inflammation-related
disorders
livestock.
Язык: Английский