Navigating gene editing in porcine embryos: Methods, challenges, and future perspectives
Genomics,
Год журнала:
2025,
Номер
117(2), С. 111014 - 111014
Опубликована: Фев. 13, 2025
Gene
editing
technologies,
particularly
CRISPR/Cas9,
have
emerged
as
transformative
tools
in
genetic
modification,
significantly
advancing
the
use
of
porcine
embryos
biomedical
and
agricultural
research.
This
review
comprehensively
examines
various
methodologies
for
gene
delivery
methods,
such
somatic
cell
nuclear
transfer
(SCNT),
microinjection,
electroporation,
lipofection.
review,
focuses
on
advantages
or
limitations
using
different
biological
sources
(in
vivo-
vs.
vitro
oocytes/embryos).
Male
germ
manipulation
sperm-mediated
(SMGT)
testis-mediated
(TMGT)
represent
innovative
approaches
producing
genetically
modified
animals.
Although
these
technologies
revolutionized
engineering
field,
all
strategies
face
challenges,
including
high
rates
off-target
events
mosaicism.
emphasizes
need
to
refine
with
a
focus
reducing
mosaicism
improving
accuracy.
Further
advancements
are
essential
unlocking
full
potential
both
applications
innovations.
Язык: Английский
Follicle mural granulosa cells encapsulated in sodium alginate gel improve developmental competence of porcine oocytes of in vitro maturation
Theriogenology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Effects of Follicular Fluid and Serum Supplementation on Cumulus Cell Expansion and Nuclear Progression of Guinea Pig Oocytes, Using a Baseline Medium Established with Bovine Oocytes
Animals,
Год журнала:
2025,
Номер
15(5), С. 666 - 666
Опубликована: Фев. 25, 2025
This
study
evaluated
the
effects
of
serum
(egpS)
and
follicular
fluid
(egpFF)
from
estrus
guinea
pigs
as
in
vitro
maturation
(IVM)
supplements
for
natural
cycle
pig
oocytes.
Two
experiments
were
conducted:
first
assessed
three
IVM
media,
a
commercial
medium
(CMOM)
two
homemade
media
(HMOM-P
HMOM-S),
oocyte
using
615
bovine
oocytes,
while
second
incorporation
5%,
10%,
20%
egpFF
or
egpS
into
1744
Initially,
we
optimized
base
oocytes
to
determine
most
suitable
culture
conditions.
The
results
obtained
these
served
critical
foundation
subsequent
supplementation
trials
conducted
with
cultured
70
μL
drops
under
controlled
atmospheric
conditions,
rates
based
on
cumulus
cell
expansion
nuclear
progression.
HMOM-S
significantly
enhanced
(72.5
±
2.88%)
compared
CMOM
(57.7
5.33%;
p
<
0.05).
Supplementation
at
5%
(68.8
6.22%),
10%
(76.3
5.39%),
(80.9
6.22%)
improved
both
progression
high-quality
(types
A
B),
control
group
(43.3
4.82%).
Conversely,
did
not
have
significant
effect
(p
>
0.05);
however,
it
notably
low-quality
(type
C)
concentrations
resulted
an
overall
improvement
outcomes,
particularly
compromised
initial
quality.
These
findings
demonstrated
that
was
by
presence
egpFF,
whereas
exhibited
less
pronounced
outcomes.
Язык: Английский
Pig
Elsevier eBooks,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Melatonin Supplementation does not improve Ovine Pre- or Post-Hatching Development in Vitro
Theriogenology,
Год журнала:
2025,
Номер
unknown, С. 117428 - 117428
Опубликована: Апрель 1, 2025
Язык: Английский
Molecular Mechanisms of Somatic Cell Cloning and Other Assisted Reproductive Technologies in Mammals: Which Determinants Have Been Unraveled Thus Far?—Current Status, Further Progress and Future Challenges
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13675 - 13675
Опубликована: Дек. 21, 2024
Taking
into
consideration
recent
reports
on
the
successful
creation
of
cloned
rhesus
monkeys
[...]
Язык: Английский