
Animals, Год журнала: 2025, Номер 15(9), С. 1242 - 1242
Опубликована: Апрель 28, 2025
This study aimed to investigate the effects of adding different levels punicalagin or oleuropein TRIS diluent on quality frozen-thawed semen from Najdi rams. Semen was diluted using TRIS-based diluter with 15% egg yolk (control group); supplemented 0.1, 0.5, 1 mg/100 mL (in Experiment 1); 1, 2.5, 5 2). The collected evaluated and cryopreserved, motility concentration sperm assessed a CASA system. results showed that total motile spermatozoa (TMS), percentage progressive (PMS), curvilinear velocity (VCL), rectilinear velocity, average path (VAP), linearity coefficient, straightness index, minor defects, vitality were higher in 0.1 group (p < 0.05) than other groups. HOST% post-thawing significantly all groups compared control 0.001). percentages PMS, TMS, VCL, Oleuropein supplementation at decreased VAP cooled sperms, while increased post-thawing. HOST-positive sperms% oleuropein-treated Moreover, nonsignificantly acrosome integrity studied concentrations (2.5 mL) frozen sperms. In conclusion, (0.1 (1 improved
Язык: Английский