PacBio Full-Length and Illumina Transcriptomes of the Gill Reveal the Molecular Response of Corbicula fluminea under Aerial Exposure

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11474 - 11474

Опубликована: Сен. 29, 2022

Air exposure is a common stress for Corbicula fluminea, an economically important freshwater shellfish consumed in China, during aquaculture and transportation. However, little known about its molecular responses to air exposure. Therefore, this study used combination of PacBio full-length Illumina transcriptomes investigate A total 36,772 transcripts were obtained using sequencing. Structural analysis identified 32,069 coding sequences, 1906 transcription factors, 8873 simple sequence repeats, 17,815 long non-coding RNAs. Subcellular localization showed that most located the cytoplasm nucleus. After 96-h exposure, 210 differentially expressed genes (DEGs) gill via Among these DEGs, related glycolysis, tricarboxylic acid cycle, lipid metabolism, amino metabolism upregulated. Additionally, many DEGs associated with immunity, cytoskeleton reorganization, autophagy, ferroptosis identified. These findings indicated metabolic strategy change, immune response, reconstruction, might be mechanisms C. fluminea use cope This will enrich gene resources provide valuable data studying coping other mollusks.

Язык: Английский

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Utilization of Genome Editing for Livestock Resilience in Changing Environment

Black Sea Journal of Agriculture, Год журнала: 2023, Номер 6(3), С. 314 - 320

Опубликована: Март 31, 2023

Climate change poses a significant threat to livestock production systems, including changes in temperature and rainfall patterns, increased frequency of extreme weather events, the spread diseases. The use genome editing technologies presents potential solution mitigate impacts climate on livestock. This paper reviewed prospects utilizing mitigating impact Applications development heat-tolerant, disease-resistant as well animals with improved feed water efficiency reduced methane emissions are explored. Additionally, breeding program for gene edited is proposed. There several different techniques that can be used breeding, CRISPR/Cas9, TALENs, zinc-finger nucleases. These involve introducing specific animal's genome, such deleting or replacing genes, new ones. technology has enormous improving it allows creation desirable traits much shorter time frame than traditional methods. Generally, may take years even decades breed an animal trait using methods, whereas achieve same result just few generations. Genome by reducing conversion modifying genes responsible production. Technology utilized improve feeds involved plant growth, development, nutrient use. lead forages high yielding, more nutritious better adapted diverse environments. resistant diseases, which help reduce need antibiotics other treatments. particularly important given growing problem antibiotic resistance, major concern both human health. developing thermo-tolerant, efficiency. proposed gene-edited will ensure produced healthy, genetically diverse, meet desired traits. In terms ethical concerns, policies ought consider unintended consequences characteristics viewed undesirable unethical. Overall, revolutionize contribute global effort change.

Язык: Английский

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Examination of the potential roles of insulin-like peptide receptor in regulating the growth of Manila clam Ruditapes philippinarum

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Год журнала: 2022, Номер 274, С. 111315 - 111315

Опубликована: Сен. 9, 2022

Язык: Английский

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Effects of High Temperature and Low-Salinity Stress on Physiology and Metabolism in the Manila Clam Ruditapes Philippinarum

Опубликована: Янв. 1, 2024

Under global climate change, various environmental stress may affect growth, survival, reproduction, and metabolism of marine bivalves. The Manila clam Ruditapes philippinarum is a commercially important bivalve in China. Recently, R. are usually stressed by high temperatures low salinity during summer seasons, resulting mass mortality. Therefore, there an urgent need to reveal the combined effects factors on physiology philippinarum. To temperature low-salinity philippinarum, we analyzed cellular molecular responses under (30°C) (15, 23) after exposure 24 h. With prolongation stress, mortality rate was(94 ± 1.0) % at h under(temperature 30°C 15), clams survival begin show decline at(temperature 23). After high-temperature tissue structural damage was observed hepatopancreatic tissue, such as epithelial cell crumpling, reduction number, vacuolation. Meantime, activities antioxidant enzymes (CAT, SOD, GST) were significantly changed 12 exposure, showing GST activity highly elevated compared with control group (P < 0.01). As result, affected expression levels energy metabolism-related genes (Se-GPx, GR, MnSOD, HK1, ADPGK, Hsp70). Among these genes, MnSOD all experimental groups 0.01).The results indicate that combination short-term can morphology, capacity this study will provide scientific basis for elucidating underlying

Язык: Английский

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Integrated Physiological, Transcriptomics and Metabolomics Analyses Provide Insights into Thermal Resistance of Razor Clam, Sinonovacula Constricta

Опубликована: Янв. 1, 2024

Язык: Английский

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