PLoS ONE,
Год журнала:
2011,
Номер
6(11), С. e26680 - e26680
Опубликована: Ноя. 7, 2011
Background
Effective
innate
responses
against
potential
pathogens
are
essential
in
the
living
world
and
possibly
contributed
to
evolutionary
success
of
invertebrates.
Taken
together,
antimicrobial
peptide
(AMP)
precursors
defensin,
mytilin,
myticin
mytimycin
can
represent
about
40%
hemocyte
transcriptome
mussels
injected
with
viral-like
bacterial
preparations,
unique
profiles
C
variants
expressed
single
mussels.
Based
on
amplicon
pyrosequencing,
we
have
ascertained
compared
natural
Vibrio-induced
diversity
AMP
transcripts
mussel
hemocytes
from
three
European
regions.
Methodology/Principal
Findings
Hemolymph
was
collected
farmed
coastal
regions
Palavas
(France),
Vigo
(Spain)
Venice
(Italy).
To
families
known
M.
galloprovincialis,
nine
transcript
sequences
been
selected,
amplified
RNA
subjected
pyrosequencing.
(offshore)
wild
(lagoon)
mussels,
both
107
Vibrio
cells,
were
similarly
processed.
Amplicon
pyrosequencing
emphasized
diversity,
Single
Nucleotide
Changes
(SNC)
minimal
for
mytilin
B/C
maximal
arthropod-like
defensin
C.
Ratio
non-synonymous
vs.
synonymous
changes
also
greatly
differed
between
isotypes.
Overall,
each
revealed
similar
levels
nucleotidic
variation
across
geographical
regions,
two
main
sequence
patterns
confirmed
no
substantial
after
immunostimulation.
Conclusions/Significance
Barcoding
bidirectional
allowed
us
map
compare
AMPs.
Though
most
genuine
cds
common
analyzed
samples
could
estimate
9
106
hemolymph
pools
representing
100
depending
isoform
sampling
site.
In
this
study,
prevailing
SNC
related
origin
or
injection
emerged.
Whether
not
contact
increase
amount
requires
additional
study.
Environmental Toxicology and Chemistry,
Год журнала:
2025,
Номер
44(2), С. 306 - 317
Опубликована: Янв. 6, 2025
Histological
evaluations
of
tissues
are
commonly
used
in
environmental
monitoring
studies
to
assess
the
health
and
fitness
status
populations
or
even
whole
ecosystems.
Although
traditional
histology
can
be
cost-effective,
there
is
a
shortage
proficient
histopathologists
results
often
subjective
between
operators,
leading
variance.
Digital
pathology
powerful
diagnostic
tool
that
has
already
significantly
transformed
research
human
but
rarely
been
applied
studies.
analyses
slide
images
introduce
possibilities
highly
standardized
histopathological
evaluations,
as
well
use
artificial
intelligence
for
novel
analyses.
Furthermore,
incorporation
digital
into
using
bioindicator
species
groups
such
bivalves
fish
greatly
improve
accuracy,
reproducibility,
efficiency
This
review
aims
readers
how
it
includes
guidelines
sample
preparation,
potential
sources
error,
comparisons
Environmental Science & Technology,
Год журнала:
2011,
Номер
45(6), С. 2368 - 2376
Опубликована: Фев. 15, 2011
Municipal
wastewater
effluents
(MWWEs)
represent
one
of
the
largest
point
sources
contamination,
but
few
studies
have
addressed
impact
on
fish
populations.
We
tested
hypothesis
that
MWWEs
disrupt
multiple
stress-related
pathways
by
examining
expression
genes
and
proteins
in
rainbow
trout
(Oncorhynchus
mykiss).
A
caging
study
was
undertaken
placing
juvenile
for
14
d
either
at
an
upstream
control
or
100%,
50%,
10%
MWWE
sites.
custom-made
low-density
cDNA
microarray
utilized
transcriptomics,
select
gene
confirmed
with
quantitative
real-time
PCR.
exposure
significantly
elevated
plasma
cortisol,
glucose,
vitellogenin
levels,
altered
a
number
hepatic
genes.
Notably,
genes,
hormone
receptors,
glucose
transporter
2,
related
to
immune
function
were
altered.
The
protein
glucocorticoid
receptor,
heat
shock
70
90,
cytochrome
P4501A1
also
impacted
exposure.
Our
results
demonstrate
tertiary-treated
elicit
organismal
cellular
stress
response
may
lead
enhanced
energy
demand
exposed
fish.
disruption
suggests
reduce
performance
subsequent
stressors.
PLoS ONE,
Год журнала:
2015,
Номер
10(6), С. e0128488 - e0128488
Опубликована: Июнь 4, 2015
Freshwater
mussels
(Family
Unionidae)
are
among
the
most
imperiled
group
of
organisms
in
world,
with
nearly
65%
North
American
species
considered
endangered.
Anthropogenic
disturbances,
including
altered
flow
regimes,
habitat
alteration,
and
pollution,
major
driver
this
group's
decline.
We
investigated
effects
tertiary
treated
municipal
wastewater
effluent
on
survivorship,
growth,
condition
freshwater
experimental
cages
a
small
Central
Texas
stream.
tested
by
measuring
basic
physical
parameters
native
three
ridge
(Amblema
plicata)
non-native
Asian
clams
(Corbicula
fluminea),
before
after
72-day
exposure
at
four
sites
above
below
treatment
plant
outfall.
Survivorship
growth
indices
were
significantly
higher
reference
site
outfall
than
downstream
sites.
attribute
reduction
fitness
to
elevated
nutrient
heavy
metal
concentrations,
potential
presence
other
untested-for
compounds
commonly
found
effluent.
These
results,
along
an
absence
discharge,
indicate
significant
negative
impact
both
