Application of a ImageJ-Based Method to Measure Blood Flow in Adult Zebrafish and Its Applications for Toxicological and Pharmacological Assessments
Biology,
Год журнала:
2025,
Номер
14(1), С. 51 - 51
Опубликована: Янв. 10, 2025
Blood
flow
is
an
important
physiological
endpoint
to
measure
cardiovascular
performance
in
animals.
Because
of
their
innate
transparent
bodies,
zebrafish
excellent
animal
model
for
assessing
vivo
performance.
Previously,
various
helpful
methods
measuring
blood
larvae
were
discovered
and
developed.
However,
optimized
method
adult
has
not
been
reported.
In
this
paper,
the
tail
fin
region
was
selected
as
target
measurements
using
Trackmate
method,
provided
by
ImageJ
platform.
Based
on
power
statistic
calculations,
aortic
vessel
at
base
selected,
other
parameters,
such
ambient
temperature,
investigated
standardization,
order
minimize
experimental
variation.
The
also
validated
fenpropathrin
ponatinib,
which
showed
some
cardiac
alterations
a
previous
study.
We
checked
versatility
following
same
setup
black
tetra
medaka
found
that
performed
well.
our
results
show
heavy
pigmentation,
like
tiger
barb,
overlapping
vessels,
those
parrot
fish,
make
it
hard
perform
Overall,
protocol
used
first
time
velocity
wild-type
without
aid
transgenic
lines
or
fluorescent
dye.
Язык: Английский
Combined Neurotoxic Effects of Commercial Formulations of Pyrethroid (Deltamethrin) and Neonicotinoid (Imidacloprid) Pesticides on Adult Zebrafish (Danio rerio): Behavioral, Molecular, and Histopathological Analysis
Life,
Год журнала:
2025,
Номер
15(4), С. 538 - 538
Опубликована: Март 25, 2025
The
use
of
different
commercial
products
that
involve
one
or
multiple
active
substances
with
specific
targeted-pests
control
has
become
a
widespread
practice.
Because
this,
severe
range
significant
consequences
been
often
reported.
Among
the
most
used
pesticides
worldwide
are
deltamethrin
(DM)
and
imidacloprid
(IMI).
With
significative
effect
on
insect’s
nervous
system,
DM
acts
voltage-gated
sodium
channels
in
nerve
cell
membranes,
while
IMI
mimics
acetylcholine
neurotransmitter
by
binding
irreversibly
to
nicotinic
receptors.
This
study
investigates
neurotoxic
effects
sub-chronic
exposure
formulations
(IMI)
adult
zebrafish,
both
individually
combination.
this
contain
additional
ingredients
commonly
found
pesticide
products,
which
may
contribute
overall
toxicity.
Fish
were
exposed
environmentally
relevant
concentrations
these
for
21
days,
Behavioral,
molecular,
histopathological
analyses
conducted
assess
impact
pesticides.
Zebrafish
exhibited
dose-dependent
behavioral
alterations,
particularly
combined
groups,
including
increased
erratic
swimming
anxiety-like
behavior.
Gene
expression
analysis
revealed
changes
neurotrophic
factors
(BDNF,
NGF,
ntf-3,
ntf-4/5,
ntf-6/7)
their
receptors
(ntrk1,
ntrk2a,
ntrk2b,
ntrk3a,
ntrk3b,
ngfra,
ngfrb),
indicating
potential
effects.
Histopathological
examination
confirmed
neuronal
degeneration,
gliosis,
vacuolization,
more
impairments
observed
mixture
treatments.
These
findings
highlight
aquatic
environments
emphasize
need
stricter
regulations
mixtures
further
research
interactions.
Our
combination
could
trigger
synergistic
maximizing
toxicity
each
compound.
Thus,
it
is
well-known
practice
pyrethroids
neonicotinoids
be
together
agriculture.
Even
so,
its
prevalence
agriculture
investigate
actual
human
health,
biodiversity,
ecosystem
mitigates
development
new
strategies
assessing
risk
and,
at
same
time,
enhancing
effectiveness.
Язык: Английский
Exploring the CD3/CD56/TNF-α/Caspase3 pathway in pyrethroid-induced immune dysregulation: curcumin-loaded chitosan nanoparticle intervention
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 6, 2025
Conflict
reports
exist
on
the
impact
of
pyrethroid
insecticides
immune
function
and
probable
underlying
mechanisms.
This
study
evaluated
effect
an
extensively
used
insecticide,
fenpropathrin
(FTN)
(15
mg/kg
b.wt),
innate
humoral
components,
blood
cells,
splenic
oxidative
status,
mRNA
expression
CD3,
CD20,
CD56,
CD8,
CD4,
IL-6,
TNF-α,
Caspase3
in
a
60-day
trial
rats.
Besides,
possible
defensive
curcumin-loaded
chitosan
nanoparticle
(CML-CNP)
(50
b.wt)
was
evaluated.
FTN
exposure
resulted
hypochromic
normocytic
anemia,
thrombocytosis,
leukocytosis,
lymphopenia.
significant
reduction
IgG,
not
IgM,
but
increased
C3
serum
levels
evident
FTN-exposed
Moreover,
their
tissues
displayed
substantial
increase
ROS,
MDA,
IL-1β
content,
altered
histology,
reduced
GPX,
GSH,
GSH/GSSG.
Furthermore,
upregulation
downregulation
CD8
detected
significantly
upregulated
Caspase-3
its
immunohistochemical
expression,
along
with
elevated
TNF-α
immunoexpression.
However,
alterations
function,
antioxidant
cell
populations,
immune-related
gene
were
notably
restored
+
CML-CNP-treated
group.
The
findings
this
highlighted
immunosuppressive
effects
suggested
involvement
many
CD
markers
as
potential
mechanism.
Additionally,
results
demonstrated
effectiveness
CML-CNP
mitigating
pollutant-induced
disorders.
Язык: Английский
The impacts of pesticide exposure on fish conspecific interactions: A systematic review and meta-analysis
Environmental Pollution,
Год журнала:
2025,
Номер
unknown, С. 126353 - 126353
Опубликована: Май 1, 2025
Язык: Английский
Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology,
Год журнала:
2024,
Номер
283, С. 109969 - 109969
Опубликована: Июнь 24, 2024
Язык: Английский
The mechanistic pathway induced by fenpropathrin toxicity: Oxidative stress, signaling pathway, and mitochondrial damage
Journal of Biochemical and Molecular Toxicology,
Год журнала:
2024,
Номер
38(11)
Опубликована: Окт. 17, 2024
Abstract
Fenpropathrin
(FNP)
is
a
kind
of
insecticide
and
acaricide
known
as
pyrethroid.
It
very
effective,
has
wide
range
activities,
works
quickly.
Internationally,
it
commonly
considered
the
most
powerful
pyrethroid
insecticide.
Nevertheless,
an
increasing
amount
data
indicates
substantial
link
between
adverse
effects
on
nontarget
species,
including
liver
toxicity,
kidney
nerve
damage,
reproductive
toxicity.
Oxidative
stress
plays
vital
role
in
toxicity
fenpropathrin,
addition
to
its
mechanical
mechanism.
This
study
offers
thorough
examination
harmful
oxidative
mitochondrial
processes,
well
signaling
pathways
involved
these
effects.
The
significant
impact
emphasizes
Fenpropathrin.
Язык: Английский
Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 30, 2024
Abstract
Carbofuran
(CF)
is
a
carbamate
class
pesticide,
widely
used
in
agriculture
for
pest
control
crops.
On
the
other
hand,
CF
also
detected
as
contaminant
food
and
water
sources.
This
pesticide
has
high
toxicity
non-target
organisms,
its
presence
environment
poses
threat
to
ecosystem.
Research
revealed
that
this
acts
an
inhibitor
of
acetylcholinesterase
(AChE),
inducing
accumulation
acetylcholine
brain.
Consequently,
leads
emergence
cholinergic
syndrome
various
detrimental
effects
on
human
health.
Nonetheless,
our
understanding
impact
central
nervous
system
remains
elusive.
Therefore,
study
aimed
investigate
behavioral
neurochemical
parameters
adult
zebrafish.
The
animals
were
exposed
96
hours
different
concentrations
(5,
50,
500
µg/L)
subjected
assessments
novel
tank
test
(NTT)
social
preference
(SPT).
Subsequently,
they
euthanized,
their
brains
evaluate
markers
associated
with
oxidative
stress
AChE
levels.
In
NTT
SPT,
did
not
alter
evaluated
parameters.
Furthermore,
affect
levels
AChE,
non-protein
sulfhydryl
groups,
thiobarbituric
acid
reactive
species
zebrafish
However,
further
research
needed
regarding
environmental
exposure
compound
organisms.
Язык: Английский
An update of the impact of pesticide exposure on memory and learning
Deleted Journal,
Год журнала:
2024,
Номер
1(1)
Опубликована: Ноя. 11, 2024
The
dependence
on
the
use
of
pesticides
in
agribusiness
leads
to
an
increase
human
exposure
these
chemical
compounds
through
occupational
activity,
contamination
environment,
food
and
water.
nervous
system
is
target
most
insecticidal
when
are
poorly
selective,
they
can
harm
non-target
species,
including
humans.
This
work
aimed
investigate
effects
pesticide
memory
learning.
Articles
published
PubMed
database
between
2015
2024
were
reviewed.
One
hundred
sixty-one
articles
included
this
review,
which
one
thirty
two
preclinical
studies
twenty-nine
studies.
Among
studies,
organophosphates,
pyrethroids,
neonicotinoids,
carbamates,
addition
herbicides,
fungicides
mixed
exposures
was
associated
with
learning
deficits
animals
such
as
bees,
mosquitoes,
fishes,
mice,
rats,
bats.
Olfactory,
associative
aversive
learning,
well
capacity
affected
by
pesticides.
Most
evaluated
environmental
organophosphate
pesticides,
pyrethroids
carbamates
outcomes
interest.
Living
close
application
areas
having
parents
working
agriculture
worse
scores
childhood
adolescence.
Prenatal
insecticides
has
already
been
poorer
attention
skills
attention-deficit/hyperactivity
disorder
(ADHD)
symptoms,
intellectual
disability,
autism
spectrum
disorder,
motor
delays,
problems.
In
adults,
during
activity
also
performance.
evidence
found
review
suggests
that
different
mechanisms
action
able
cause
some
cognitive
impairment.
Developmental
exposure,
chronic
contribute
performance,
especially
memory.
Язык: Английский