International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12495 - 12495
Опубликована: Ноя. 21, 2024
An
abdominal
aortic
aneurysm
(AAA)
is
described
as
a
gradual
and
localized
permanent
expansion
of
the
aorta
resulting
from
weakening
vascular
wall.
The
key
aspects
AAA's
progression
are
high
proteolysis
structural
elements
wall,
depletion
smooth
muscle
cells
(VSMCs),
chronic
immunoinflammatory
response.
pathological
mechanisms
underpinning
development
an
AAA
complex
still
unknown.
At
present,
there
no
successful
drug
treatments
available
that
can
slow
or
prevent
rupture
aneurysmal
Recently,
it
has
been
suggested
endothelial
cellular
senescence
may
be
involved
in
aging
diseases,
but
clear
correlation
between
AAAs.
Therefore,
aim
this
study
was
to
identify
presence
senescent
on
wall
aneurysmatic
aortas
correlate
their
distribution
with
morphological
markers
Pathological
healthy
segments
were
collected
during
repair
surgery
immediately
processed
for
histological
immunohistochemical
analyses.
Hematoxylin/eosin,
Verhoeff-van
Gieson,
Goldner's
Masson
trichrome
staining
procedures
carried
out
investigate
features
related
pathology.
Immunohistochemical
investigations
p21
Chemical and Biological Technologies in Agriculture,
Год журнала:
2025,
Номер
12(1)
Опубликована: Янв. 15, 2025
Mulberry
twigs,
a
traditional
Chinese
medicinal
and
agricultural
byproduct,
contain
bioactive
compounds
with
anti-atherosclerotic
potential.
This
study
aims
to
identify
evaluate
the
effects
of
key
in
mulberry
twig
extracts
(MTEs)
on
oxidized
low-density
lipoprotein
(ox-LDL)-induced
human
umbilical
vein
endothelial
cells
(HUVECs),
focus
understanding
how
these
modulate
oxidative
stress
related
signaling
pathways.
Biospecific
cell
extraction
ultra-performance
liquid
chromatography–tandem
mass
spectrometry
(UHPLC–MS/MS)
were
employed
screen
compounds.
Protective
assessed
by
measuring
viability,
malondialdehyde
(MDA),
superoxide
dismutase
(SOD)
levels,
along
detecting
intracellular
reactive
oxygen
species
(ROS)
using
2,
7-dichlorodihydrofluorescein
diacetate
(DCFH-DA)
dihydroethidine
(DHE)
probes.
Real-time
qPCR
Western
blotting
used
for
mRNA
protein
level
analysis.
Two
novel
active
compounds,
Kuwanon
H
Morusin,
known
G,
identified.
They
significantly
reduced
MDA
ROS
levels
while
increasing
SOD
activity
ox-LDL-treated
HUVECs.
was
particularly
effective,
enhancing
nuclear
factor
erythroid
2-related
2
(Nrf-2)
upregulating
its
target
genes
Heme
oxygenase-1
(HO-1)
NAD(P)H:
quinone
oxidoreductase
1
(NQO-1).
In
conclusion,
H,
G
effectively
protected
HUVECs
from
ox-LDL-induced
injury,
showing
strongest
protective
via
Nrf-2/HO-1
pathway.
These
hold
potential
treating
atherosclerosis
diseases.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 31, 2025
AbstractBackground:
Aging
is
associated
with
adipose
tissue
alterations,
oxidative
stress,
and
fibrosis
together
the
onset
of
cardiometabolic
complications
such
as
atherosclerosis.
Although
it
has
been
shown
that
perivascular
(PVAT)
can
participate
to
vascular
damage,
involvement
subcutaneous
(SCAT)
-
particularly
through
its
secretory
activity
in
aging
remains
poorly
understood.
We
have
previously
human
adipose-derived
stromal
cells
(ASCs)
from
SCAT
aged
women
display
senescence
stress.
hypothesized
ASC
secretome
contributes
endothelial
dysfunction,
an
early
stage
aging.
Methods:
Conditioned
media
were
prepared
ASCs
isolated
healthy
young
(<25y)
or
(>60y)
women.
The
was
analysed
added
on
coronary
artery
(HCAEC).
Using
clinical
cohorts,
we
evaluated
expression
C-C-chemokine-ligand-5
(CCL5)/Regulated
upon
Activation
Normally
T-expressed
secreted
(RANTES)
individuals
heart
disease.
Results:
aged-donor
induced
cell
dysfunction
HCAEC,
characterized
by
lower
nitric
oxide
production,
higher
(greater
p16
expression),
a
pro-adherent
phenotype
(elevated
adhesion
molecule
secretion).
Aged-donor
also
favored
Endothelial-to-mesenchymal
transition,
characterised
mesenchymal
markers
pro-migratory
profile.
showed
CCL5/RANTES
at
level
aged-
vs.
young-donor
responsible
for
these
effects.
Accordingly,
SCAT,
but
not
epicardial
tissue,
blood
pressure
patients
diseases,
thus
confirming
important
role
disorders.
Moreover,
both
HCAEC
cohort
receptor
antagonist
drug
maraviroc
prevented
deleterious
impact
CCL5/RANTES.
Conclusions:
Our
results
highlighted
ability
released
and,
specifically,
cells,
induce
which
are
steps
potential
link
between
phenomena
hypertension
particular.
Biomolecules,
Год журнала:
2025,
Номер
15(4), С. 545 - 545
Опубликована: Апрель 8, 2025
Aging
is
a
predominant
risk
factor
for
cardiovascular
diseases.
There
evidence
demonstrating
that
senescent
cells
not
only
play
significant
role
in
organism
aging
but
also
contribute
to
the
pathogenesis
of
diseases
younger
ages.
Encouraged
by
recent
findings
elimination
pharmacogenetic
tools
could
slow
down
and
even
reverse
animal
models,
senolytic
drugs
have
been
developed,
translation
results
from
basic
research
clinical
settings
has
initiated.
Because
numerous
studies
literature
show
beneficial
therapeutic
effects
targeting
cardiomyopathies
associated
with
ischemia/reperfusion
atherosclerotic
vascular
disease,
are
considered
next
generation
therapies
disorders.
However,
reported
controversial
or
detrimental
caused
approaches,
including
worsening
cardiac
dysfunction,
instability
plaques,
an
increase
mortality
which
challenges
therapy
into
practice.
This
brief
review
article
will
focus
on
(1)
analyzing
discussing
approaches
(2)
future
directions
questions
essential
understand
controversies
translate
preclinical
The Journals of Gerontology Series A,
Год журнала:
2024,
Номер
79(11)
Опубликована: Окт. 24, 2024
Abstract
The
implication
of
Torquetenovirus
(TTV)
in
ischemic
heart
disease
(IHD)
has
not
been
thoroughly
explored.
This
study
investigated
the
association
between
TTV
viremia,
pro-inflammatory
cytokines,
and
IHD
risk
an
aging
population.
cross-sectional
included
900
non-IHD
subjects
86
individuals
with
(aged
55–75
years)
selected
from
MARK-AGE
project.
Results
were
verified
another
independent
Report-Age
cohort,
including
94
inpatients
chronic
111
65–96
years).
Multivariable
logistic
regression
cohort
revealed
that
male
sex,
viremia
≥4log,
Cu/Zn
ratio,
diabetes,
hypertension,
smoking
significant
predictors.
Notably,
≥4log
independently
increased
(odds
ratio
[OR]:
2.51,
95%
confidence
interval
[CI]:
1.42–4.43),
confirmed
(OR:
4.90,
CI:
2.32–10.39).
In
a
RASIG
subgroup,
≥4
log,
both
without
IHD,
exhibited
plasma
cytokine
levels
(IFN-γ,
IL-1β,
IL-6,
IL-10,
IL-12p70,
TNF-α)
compared
to
those
<4
log.
No
difference
production
was
observed
patients
A
positive
correlation
DNA
methylation
estimator
leukocyte
telomere
length
patients.
Additionally,
log
displayed
higher
NLR
SIRI
index
than
conclusion,
high
is
associated
elevated
older
population,
potentially
arising
augmented
response
immunosenescence.
