Vascular Mesenchymal Stromal Cells and Cellular Senescence: A Two-Case Study Investigating the Correlation Between an Inflammatory Microenvironment and Abdominal Aortic Aneurysm Development DOI Open Access
Gabriella Teti,

Riccardo Camiletti,

Valentina Gatta

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12495 - 12495

Опубликована: Ноя. 21, 2024

An abdominal aortic aneurysm (AAA) is described as a gradual and localized permanent expansion of the aorta resulting from weakening vascular wall. The key aspects AAA's progression are high proteolysis structural elements wall, depletion smooth muscle cells (VSMCs), chronic immunoinflammatory response. pathological mechanisms underpinning development an AAA complex still unknown. At present, there no successful drug treatments available that can slow or prevent rupture aneurysmal Recently, it has been suggested endothelial cellular senescence may be involved in aging diseases, but clear correlation between AAAs. Therefore, aim this study was to identify presence senescent on wall aneurysmatic aortas correlate their distribution with morphological markers Pathological healthy segments were collected during repair surgery immediately processed for histological immunohistochemical analyses. Hematoxylin/eosin, Verhoeff-van Gieson, Goldner's Masson trichrome staining procedures carried out investigate features related pathology. Immunohistochemical investigations p21

Язык: Английский

Lipids and lipid metabolism in cellular senescence: Emerging targets for age-related diseases DOI

Qing Zeng,

Yongzhen Gong,

Neng Zhu

и другие.

Ageing Research Reviews, Год журнала: 2024, Номер 97, С. 102294 - 102294

Опубликована: Апрель 5, 2024

Язык: Английский

Процитировано

41

Targeting Senescent Cells in Atherosclerosis: Pathways to Novel Therapies DOI

Yuhan Tian,

Sihang Shao,

Haibo Feng

и другие.

Ageing Research Reviews, Год журнала: 2024, Номер 101, С. 102502 - 102502

Опубликована: Сен. 13, 2024

Язык: Английский

Процитировано

3

Identification of compounds against atherosclerosis induced by ox-LDL based on cell extraction/UPLC–MS/MS from mulberry twigs and their mechanistic analysis DOI Creative Commons
Zhihui Jin, Wei Xiang, Xiaowen Shi

и другие.

Chemical and Biological Technologies in Agriculture, Год журнала: 2025, Номер 12(1)

Опубликована: Янв. 15, 2025

Mulberry twigs, a traditional Chinese medicinal and agricultural byproduct, contain bioactive compounds with anti-atherosclerotic potential. This study aims to identify evaluate the effects of key in mulberry twig extracts (MTEs) on oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs), focus understanding how these modulate oxidative stress related signaling pathways. Biospecific cell extraction ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) were employed screen compounds. Protective assessed by measuring viability, malondialdehyde (MDA), superoxide dismutase (SOD) levels, along detecting intracellular reactive oxygen species (ROS) using 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) dihydroethidine (DHE) probes. Real-time qPCR Western blotting used for mRNA protein level analysis. Two novel active compounds, Kuwanon H Morusin, known G, identified. They significantly reduced MDA ROS levels while increasing SOD activity ox-LDL-treated HUVECs. was particularly effective, enhancing nuclear factor erythroid 2-related 2 (Nrf-2) upregulating its target genes Heme oxygenase-1 (HO-1) NAD(P)H: quinone oxidoreductase 1 (NQO-1). In conclusion, H, G effectively protected HUVECs from ox-LDL-induced injury, showing strongest protective via Nrf-2/HO-1 pathway. These hold potential treating atherosclerosis diseases.

Язык: Английский

Процитировано

0

C-C chemokine ligand 5 from subcutaneous adipose tissue has a central role in vascular aging DOI Creative Commons
Laura Pelletier,

Kenza Ngono Ayissi,

Émilie Capel

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 31, 2025

Abstract Background: Aging is associated with adipose tissue alterations, oxidative stress, and fibrosis together the onset of cardiometabolic complications such as atherosclerosis. Although it has been shown that perivascular (PVAT) can participate to vascular damage, involvement subcutaneous (SCAT) - particularly through its secretory activity in aging remains poorly understood. We have previously human adipose-derived stromal cells (ASCs) from SCAT aged women display senescence stress. hypothesized ASC secretome contributes endothelial dysfunction, an early stage aging. Methods: Conditioned media were prepared ASCs isolated healthy young (<25y) or (>60y) women. The was analysed added on coronary artery (HCAEC). Using clinical cohorts, we evaluated expression C-C-chemokine-ligand-5 (CCL5)/Regulated upon Activation Normally T-expressed secreted (RANTES) individuals heart disease. Results: aged-donor induced cell dysfunction HCAEC, characterized by lower nitric oxide production, higher (greater p16 expression), a pro-adherent phenotype (elevated adhesion molecule secretion). Aged-donor also favored Endothelial-to-mesenchymal transition, characterised mesenchymal markers pro-migratory profile. showed CCL5/RANTES at level aged- vs. young-donor responsible for these effects. Accordingly, SCAT, but not epicardial tissue, blood pressure patients diseases, thus confirming important role disorders. Moreover, both HCAEC cohort receptor antagonist drug maraviroc prevented deleterious impact CCL5/RANTES. Conclusions: Our results highlighted ability released and, specifically, cells, induce which are steps potential link between phenomena hypertension particular.

Язык: Английский

Процитировано

0

Unveiling the role of GATA4 in endothelial cell senescence and atherosclerosis development DOI
Chun‐Min Kang, Jingjing Zhao,

Xixi Xie

и другие.

Atherosclerosis, Год журнала: 2025, Номер 404, С. 119183 - 119183

Опубликована: Апрель 5, 2025

Язык: Английский

Процитировано

0

Is Senolytic Therapy in Cardiovascular Diseases Ready for Translation to Clinics? DOI Creative Commons
Zhihong Yang, Duilio Michele Potenza, Xiu‐Fen Ming

и другие.

Biomolecules, Год журнала: 2025, Номер 15(4), С. 545 - 545

Опубликована: Апрель 8, 2025

Aging is a predominant risk factor for cardiovascular diseases. There evidence demonstrating that senescent cells not only play significant role in organism aging but also contribute to the pathogenesis of diseases younger ages. Encouraged by recent findings elimination pharmacogenetic tools could slow down and even reverse animal models, senolytic drugs have been developed, translation results from basic research clinical settings has initiated. Because numerous studies literature show beneficial therapeutic effects targeting cardiomyopathies associated with ischemia/reperfusion atherosclerotic vascular disease, are considered next generation therapies disorders. However, reported controversial or detrimental caused approaches, including worsening cardiac dysfunction, instability plaques, an increase mortality which challenges therapy into practice. This brief review article will focus on (1) analyzing discussing approaches (2) future directions questions essential understand controversies translate preclinical

Язык: Английский

Процитировано

0

Association between TTV viremia, chronic inflammation, and ischemic heart disease risk: Insights from MARK-AGE and Report-Age projects DOI Creative Commons
Robertina Giacconi, Francesco Piacenza, Fabrizio Maggi

и другие.

The Journals of Gerontology Series A, Год журнала: 2024, Номер 79(11)

Опубликована: Окт. 24, 2024

Abstract The implication of Torquetenovirus (TTV) in ischemic heart disease (IHD) has not been thoroughly explored. This study investigated the association between TTV viremia, pro-inflammatory cytokines, and IHD risk an aging population. cross-sectional included 900 non-IHD subjects 86 individuals with (aged 55–75 years) selected from MARK-AGE project. Results were verified another independent Report-Age cohort, including 94 inpatients chronic 111 65–96 years). Multivariable logistic regression cohort revealed that male sex, viremia ≥4log, Cu/Zn ratio, diabetes, hypertension, smoking significant predictors. Notably, ≥4log independently increased (odds ratio [OR]: 2.51, 95% confidence interval [CI]: 1.42–4.43), confirmed (OR: 4.90, CI: 2.32–10.39). In a RASIG subgroup, ≥4 log, both without IHD, exhibited plasma cytokine levels (IFN-γ, IL-1β, IL-6, IL-10, IL-12p70, TNF-α) compared to those &lt;4 log. No difference production was observed patients A positive correlation DNA methylation estimator leukocyte telomere length patients. Additionally, log displayed higher NLR SIRI index than conclusion, high is associated elevated older population, potentially arising augmented response immunosenescence.

Язык: Английский

Процитировано

2

TFPI2 hypermethylation promotes diabetic atherosclerosis progression through the Ap2α/PPARγ axis DOI Creative Commons

Zongyi Xia,

Chi Zhou,

Yefeng Hong

и другие.

Journal of Molecular and Cellular Cardiology, Год журнала: 2024, Номер 198, С. 45 - 59

Опубликована: Дек. 3, 2024

Diabetes mellitus significantly escalates the risk of accelerated atherosclerosis (AS), severely affecting cardiovascular health. Our research, leveraging Gene Expression Omnibus (GEO) database analysis (GSE118481), revealed diminished TFPI2 expression in diabetic patients' atherosclerotic plaques. Further validation carotid artery plaques and an AS mouse model confirmed TFPI2's reduced diabetes. Through knockdown non-diabetic mice, we observed aggravated plaque burden increased inflammatory M1 macrophage polarization. Conversely, overexpression mice improved stability induced reparative M2 polarization, countering hyperglycemia's negative effects. Mechanistically, transcription factor activator protein 2α (AP-2α) is a repressor PPPARg transcription, interaction with AP-2α blocks binding to PPARγ gene promoter, which essential for PPARγ-mediated transition from macrophages. Additionally, hyperglycemia-induced DNA methyltransferase 1 (DNMT1) upregulation heightens methylation, reducing its expression. findings spotlight TFPI2/AP-2α/PPARγ axis as crucial modulation, proposing targeting new therapeutic strategy halt diabetes-driven progression, highlighting promise addressing diabetes-related issues.

Язык: Английский

Процитировано

2

Retrotransposon SINEs in Age-Related Diseases: Mechanisms and Therapeutic Implications DOI

Suleman Shah,

Siyi Yu, Chen Zhang

и другие.

Ageing Research Reviews, Год журнала: 2024, Номер 101, С. 102539 - 102539

Опубликована: Окт. 11, 2024

Язык: Английский

Процитировано

0

AQP1 mediates pancreatic β cell senescence induced by metobolic stress through modulating intracellular H2O2 level DOI Creative Commons

Qihui Yan,

Haifeng Zhang, Yunxiao Ma

и другие.

Free Radical Biology and Medicine, Год журнала: 2024, Номер 226, С. 171 - 184

Опубликована: Ноя. 16, 2024

Язык: Английский

Процитировано

0