Integration of Phytochemical Profiling and Computational Approaches to Evaluate the Neuroprotective Potential of Nardostachys jatamansi in Alzheimer's Disease

Biotechnology Reports, Год журнала: 2025, Номер 45, С. e00881 - e00881

Опубликована: Фев. 8, 2025

Despite broad spectrum utility of Nardostachys jatamansi (D. Don) DC, little is known about the molecular processes that underlie its anti-Alzheimer action. To investigate targets and therapeutic potential N. for Alzheimer's disease (AD), we used Gas Chromatography-Mass Spectrometry (GC-MS), ADMET analysis, network pharmacology, differential gene expression docking, dynamics (MD) simulations. The STITCH database was creation protein-protein interaction while Cytoscape visualization Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment Gene Ontology (GO) term enrichment. Additionally, to intermolecular interactions between active chemicals target proteins, docking experiments were conducted using Blind on Achilles server. stability PS1 complex with Spirojatamol, further evaluated MD With Spirojatamol showing highest binding energy scores against (-6.9 kcal/mol), confirmed activity this metabolite AD formed a stable at 100 nanoseconds, according additional investigation Significant ligand-protein verified by free calculations MM/GBSA technique. PS1-Spirojatamol had ΔG: -36.95 ± 5.00 kcal/mol. By focusing several genes pathways, involved in AD, work reveals underpinnings behind possible use treatment AD.

Язык: Английский

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Unlocking the Neuroprotective Potential of Silymarin: A Promising Ally in Safeguarding the Brain from Alzheimer’s Disease and Other Neurological Disorders

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Язык: Английский

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Semaglutide Ameliorates Neuroinflammation Caused by Enterogenous Pyrogen in APP/PS1 Mice

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Background and purpose: Alzheimer's disease (AD) is a neurodegenerative characterized by progressive cognitive dysfunction, which common in the elderly. In recent years, it has been reported that glucagon-like peptide 1 (GLP-1) analogues have neuroprotective function. However, mechanism of GLP-1 improving neurological function not fully clarified. This study attempts to clarify alleviating AD phenotype. Methods: this study, modified once-weekly analogue, Semaglutide, was used treat 8-month-old amyloid precursor protein / presenilin (APP/PS1) transgenic mice. By means ethology, molecular biology 16s rRNA amplicon sequencing, confirmed Semaglutide alleviated phenotype APP/PS1 Results: improved behavioral performance mice, reduced neuronal damage aggregation amyloid-β (Aβ) plaques, enhanced synaptic plasticity. also attenuated pyroptosis mediated NOD-like receptor thermal domain associated 3 (NLRP3), inflammatory reaction toll-like 4 (TLR4) mitochondrial microglia as well structure blood-brain barrier (BBB) Conclusion: may repair alleviate central nervous system injury caused displacement pyrogen gut

Язык: Английский

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Neuroprotective effects of Indole 3-carbinol against Scopolamine-Induced cognitive and memory impairment in rats: modulation of oxidative stress, inflammatory and cholinergic pathways

Metabolic Brain Disease, Год журнала: 2025, Номер 40(4)

Опубликована: Март 18, 2025

Язык: Английский

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Lateralized Amplitude Low-frequency Fluctuation Alterations in Mild Cognitive Impairment as a Biomarker for Early Alzheimer’s Disease Detection

Academic Radiology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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CRISPR/Cas9-Based therapeutics as a promising strategy for management of Alzheimer’s disease: progress and prospects

Frontiers in Cellular Neuroscience, Год журнала: 2025, Номер 19

Опубликована: Апрель 7, 2025

CRISPR/Cas9 technology has revolutionized genetic and biomedical research in recent years. It enables editing modulation of gene function with an unparalleled precision effectiveness. Among the various applications prospects this technology, opportunities it offers unraveling molecular underpinnings a myriad central nervous system diseases, including neurodegenerative disorders, psychiatric conditions, developmental abnormalities, are unprecedented. In review, we highlight CRISPR/Cas9-based therapeutics as promising strategy for management Alzheimer's disease transformative impact on AD research. Further, emphasize role generating accurate models identification novel therapeutic targets, besides CRISPR-based therapies aimed at correcting AD-associated mutations modulating processes. Furthermore, delivery systems reviewed potential non-viral nanotechnology-based carriers overcoming critical limitations effective is discussed. Overall, review highlights promise intricate processes underlying development AD, discusses its limitations, ethical concerns several challenges efficient across BBB, ensuring specificity, avoiding off-target effects. This article can be helpful better understanding based approaches way forward utilizing enormous targeted, gene-specific treatments that could change trajectory debilitating incurable illness.

Язык: Английский

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Advances in Understanding Biomarkers and Treating Neurological Diseases -Role of the Cerebellar Dysfunction and Emerging Therapies

Ageing Research Reviews, Год журнала: 2024, Номер unknown, С. 102519 - 102519

Опубликована: Сен. 1, 2024

Язык: Английский

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Synthesis of novel hybrids of 1,2,3-triazoles-hydrazone: targeting cholinesterases and Alzheimer's related genes

Future Medicinal Chemistry, Год журнала: 2024, Номер 16(15), С. 1519 - 1535

Опубликована: Июнь 12, 2024

Aim: A new series of 1,2,3-triazole-hydrazone derivatives were developed to evaluate their anti-Alzheimer's activity. Materials & methods: All compounds screened toward cholinesterases via the modified Ellman's method. The toxicity assay on SH-SY5Y cells was performed using MTT assay, and expression levels GSK-3α, GSK-3β, DYRK1 CDK5 assessed in presence 6m 6p. Results: 6p; acting as mixed-type inhibitors, exhibited promising acetylcholinesterase butyrylcholinesterase inhibitory activity, respectively. demonstrated no under tested concentrations positively impacted neurodegenerative pathways. Notably, displayed a significant downregulation mRNA GSK-3β CDK5. Conclusion: target could be considered developing disease agents.

Язык: Английский

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Investigating Cortical Complexity in Mixed Dementia through Nonlinear Dynamic Analyses: A Resting-State EEG Study

Iranian Journal of Psychiatry, Год журнала: 2024, Номер unknown

Опубликована: Июнь 22, 2024

Dementia is a broad term referring to decline in problem-solving abilities, language skills, memory, and other cognitive functions degree that it significantly disrupts everyday activities. The underlying cause of dementia the impairment or loss nerve cells their connections within brain. particular symptoms experienced are contingent upon specific regions brain affected by this damage. In research, we aimed investigate nonlinear dynamics mixed demented compared healthy subjects using electroencephalogram (EEG) analysis.

Язык: Английский

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Exploring the Roles of Key Mediators IKBKE and HSPA1A in Alzheimer’s Disease and Hepatocellular Carcinoma through Bioinformatics Analysis

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 6934 - 6934

Опубликована: Июнь 25, 2024

Recent studies have hinted at a potential link between Alzheimer’s Disease (AD) and cancer. Thus, our study focused on finding genes common to AD Liver Hepatocellular Carcinoma (LIHC), assessing their promise as diagnostic indicators guiding future treatment approaches for both conditions. Our research utilized broad methodology, including differential gene expression analysis, Weighted Gene Co-expression Network Analysis (WGCNA), enrichment Receiver Operating Characteristic (ROC) curves, Kaplan–Meier plots, supplemented with immunohistochemistry data from the Human Protein Atlas (HPA) machine learning techniques, identify critical significant pathways shared LIHC. Through WGCNA, methods, we identified nine key associated AD, which served entry points LIHC analysis. Subsequent analyses revealed IKBKE HSPA1A pivotal in patients LIHC, suggesting these targets intervention indicates that could influence onset progression of by modulating infiltration levels immune cells. This lays foundation into targeted therapies based mechanisms.

Язык: Английский

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