Chronic mitochondrial fragmentation elicits a neuroprotective Warburg-like effect in Drosophila neurons DOI Creative Commons
Shlesha Richhariya, Daniel Shin, Matthias Schlichting

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Abstract Mitochondrial fission and fusion are dynamic important cellular processes, but the roles of these two very different mitochondrial forms – predominantly spherical tubular - not well-characterized in neurons animals especially aging neurons. This is because long-lived dynamics associated with neurodegenerative diseases. We used here an efficient cell type-specific CRISPR approach to knockout key fission-fusion genes disrupt within inessential clock Drosophila . Surprisingly, much more than for maintaining long-term neuronal function. Neurons survive chronic fragmentation due loss by triggering a cancer-like transcriptomic response. Warburg effect includes ATF4-mediated upregulation aerobic glycolysis gene Lactate dehydrogenase ( Ldh ), LDH essential prevent neurodegeneration deficient Opa1 These results others provide insights into intersection metabolism, neurodegeneration.

Язык: Английский

L-Lactate Administration Improved Synaptic Plasticity and Cognition in Early 3xTg-AD Mice DOI Open Access
Yaxin Wang, Jinfeng Zhao, Li Zhao

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1486 - 1486

Опубликована: Фев. 11, 2025

Synaptic plasticity impairment and behavioral deficits constitute classical pathological hallmarks in early-stage Alzheimer’s disease (AD). Emerging evidence suggests these synaptic dysfunctions may stem from metabolic dysregulation, particularly impaired aerobic glycolysis. As a key product of astrocyte-mediated glycolysis, lactate serves dual roles as both an energy substrate signaling molecule, playing critical regulatory role long-term memory formation. This study investigated whether exogenous L-lactate supplementation could ameliorate dysfunction cognitive AD models. Our findings reveal significant reductions hippocampal levels experimental mice. Systemic administration (200 mg/kg) effectively restored physiological concentrations tissue cerebrospinal fluid (CSF). Chronic treatment significantly improved spatial learning performance assessments. Electrophysiological recordings demonstrated that either acute bath application (2 mM) to slices or chronic intraperitoneal enhanced high-frequency stimulation (HFS)-induced potentiation (LTP) magnitude 3xTg-AD Ultrastructural analysis revealed density morphological features synapses. At the molecular level, upregulated marker synaptophysin (SYP) expression while downregulating activity-regulated cytoskeletal-associated protein (ARC) These multimodal demonstrate restores function mice through concurrent improvements at behavioral, structural, levels.

Язык: Английский

Процитировано

1

Lactylation: A Novel Epigenetic Regulator of Cellular Senescence DOI Creative Commons
Caiyu Sun, Jiaxuan Li, Lei Dong

и другие.

Aging and Disease, Год журнала: 2025, Номер unknown, С. 0 - 0

Опубликована: Янв. 1, 2025

Cellular senescence is the basic unit of organismal aging, a complicated biological process involving several cell types and tissues. It also an important mechanism by which body responds to damage potential carcinogenesis. However, excessive or abnormal cellular can lead tissue functional degradation occurrence diseases. In recent years, role epigenetic modifications in has received extensive attention. Lactylation, novel post-translational modification derived from lactate, recently gained significant attention as key factor metabolism regulation, gradually demonstrating its importance regulation senescence. This review emphasizes bidirectional causal relationship between lactylation senescence, highlighting therapeutic target for aging-related

Язык: Английский

Процитировано

0

Lactylation in CNS disorders: mechanisms, cellular function, and disease relevance DOI Creative Commons

Jiaxin Tian,

Tongyu Zhang, Ruidan Zhang

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Март 28, 2025

Lactate, as a metabolic product or energy substrate, participates in various neurological processes within the physiological and pathological frameworks of central nervous system (CNS). The groundbreaking application multi-omics integration technologies has unveiled novel role for lactate: lactylation, unique post-translational modification (PTM) that covalently attaches lactate groups to lysine residues on proteins. This process precisely regulates protein function gene expression, profoundly influencing progression diseases. lactylation is meticulously regulated by variety key enzymes pathways, forming dynamic intricate network. In this review, we summarize involved specifically "Writers," "Erasers," "Readers." Furthermore, systematically categorize observed cell types CNS investigate its multifaceted roles processes, including neurodegenerative diseases, brain tumors, injuries. By consolidating latest research findings field, our review aims highlight significance these discoveries future explore their potential translational applications.

Язык: Английский

Процитировано

0

UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling DOI Creative Commons

Wei‐Ren Lan,

Xueman Chen, Hong Yu

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Osteoarthritis (OA) progression is closely related to dysregulated glycolysis. As the primary metabolite of glycolysis, lactate plays a detrimental role in OA. However, how exacerbates OA process remains unclear. Here, this study revealed that levels are elevated synovial fluid patients and IL-1β-treated human chondrocytes, promoting protein pan-lactylation. Functionally, hyper-lactylation chondrocytes extracellular matrix (ECM) degradation cell apoptosis vitro vivo. Moreover, UDP-glucose dehydrogenase (UGDH) proven be key lactylated lactate-treated which undergoes lactylation at lysine 6 (K6). Lactylated UGDH repressed its enzymatic activity, reducing glycosaminoglycan synthesis disregulating nuclear-cytoplasmic distribution. Mechanistically, K6 impedes interaction signal transducer activator transcription 1 (STAT1), thus mitogen-activated kinase 8 (MAP3K8) activating MAPK signaling pathway. Importantly, vivo treatment with A485, specific acyltransferase P300 inhibitor, suppressed rescued ECM progression. These findings uncover new mechanism underlying pathogenesis highlight potential targeting as novel therapeutic strategy for

Язык: Английский

Процитировано

0

Glucose oxidase-embedded mesoporous polydopamine nanoparticles produce CO for synergistic tumor starvation, chemodynamic, and photothermal therapy DOI

Lingli Guo,

Nan Wang,

Yuqin Zhang

и другие.

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер 253, С. 114729 - 114729

Опубликована: Апрель 23, 2025

Язык: Английский

Процитировано

0

GBE1 alleviates MPTP-induced PD symptoms in mice by enhancing glycolysis and oxidative phosphorylation DOI
Hongyan Chen, Hao Ding,

Dongya Huang

и другие.

Brain Research, Год журнала: 2025, Номер unknown, С. 149663 - 149663

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Lactate links exercise to synaptic protection and cognitive enhancement in Alzheimer’s disease models DOI Creative Commons
Hao Han, Yi Wu,

Rivkin Mi

и другие.

BMC Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Июнь 3, 2025

Physical exercise is known to promote cognitive resilience in aging and Alzheimer's disease (AD), but the underlying mechanisms remain incompletely understood. Lactate, a metabolic byproduct elevated during exercise, has recently emerged as potential neuromodulator of brain function. Three AD-like mouse models (SAMP8, Aβ1-42 injection, 5xFAD) were used evaluate effects treadmill on function synaptic plasticity. Serum lactate levels assessed, sodium L-lactate (NaLA) was administered determine its neuroprotective effects. Transcriptomic analysis performed hippocampal tissue explore lactate-regulated pathways. Lactate transport pharmacologically inhibited using 4-CIN for mechanistic validation. Treadmill significantly improved performance increased synapse-associated protein expression AD models. Exercise serum levels, exogenous NaLA administration recapitulated benefits exercise. profiling revealed enrichment genes involved axon guidance organization, including upregulation multiple Eph receptor family genes. α-cyano-4-hydroxycinnamic acid (4-CIN) abolished exercise-induced effects, confirming essential role mediating these benefits. This study identifies critical mediator linking enhanced improvement models, providing insights highlighting metabolism therapeutic target AD.

Язык: Английский

Процитировано

0

Lactylation of tau in human Alzheimer's disease brains DOI Creative Commons

Xiaoyu Zhang,

Yan Liu,

Michaella J. Rekowski

и другие.

Alzheimer s & Dementia, Год журнала: 2024, Номер unknown

Опубликована: Дек. 30, 2024

Aggregation of hyperphosphorylated tau (tauopathy) is associated with cognitive impairment in patients Alzheimer's disease (AD). In AD, a metabolic shift due to the Warburg effect results increased lactate production. Lactate can induce post-translational modification (PTM) on proteins that conjugates lactyl groups lysine (K) residues, which known as lactylation.

Язык: Английский

Процитировано

2

Chronic mitochondrial fragmentation elicits a neuroprotective Warburg-like effect in Drosophila neurons DOI Creative Commons
Shlesha Richhariya, Daniel Shin, Matthias Schlichting

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Abstract Mitochondrial fission and fusion are dynamic important cellular processes, but the roles of these two very different mitochondrial forms – predominantly spherical tubular - not well-characterized in neurons animals especially aging neurons. This is because long-lived dynamics associated with neurodegenerative diseases. We used here an efficient cell type-specific CRISPR approach to knockout key fission-fusion genes disrupt within inessential clock Drosophila . Surprisingly, much more than for maintaining long-term neuronal function. Neurons survive chronic fragmentation due loss by triggering a cancer-like transcriptomic response. Warburg effect includes ATF4-mediated upregulation aerobic glycolysis gene Lactate dehydrogenase ( Ldh ), LDH essential prevent neurodegeneration deficient Opa1 These results others provide insights into intersection metabolism, neurodegeneration.

Язык: Английский

Процитировано

0