Chlorpyrifos degradation by Bacillus sp. strain UPMB10 isolated from polluted environment: Analysis and characterization of the metabolite by GC-MS

Case Studies in Chemical and Environmental Engineering, Год журнала: 2024, Номер 9, С. 100608 - 100608

Опубликована: Янв. 3, 2024

Chlorpyrifos, being a frequently used organophosphate pesticide poses significant threat to human health due its prolonged presence in soil, plants, and water, this has raised concern. Consequently, researchers have shifted their attention towards finding methods mitigate toxicity or eliminate it from the environment. This highlighted importance of bioremediation. study was aimed at analyzing characterizing metabolites chlorpyrifos degradation by Bacillus sp. strain UPMB10 isolated polluted environment using Gas Chromatography-Mass Spectrometry. The bacteria were successfully utilizing enrichment technique mineral salt media, followed screening obtain best isolate. Identification isolate done morphological, biochemical, molecular levels, with focus on 16S rRNA gene. Phylogenetic analysis revealed close relationship between bacterium UPMB10. Optimization (One Factor A Time) determine growth conditions optimal obtained include, incubation time 48 hours, substrate concentration 4 g/L, pH 7.0, temperature 37 °C, an inoculum size 2 % (v/v), these quantitative data valuable insights into factors affecting efficient pesticide. Spectrometry demonstrated that achieved remarkable efficiency 99 after hours incubation. Notably, Mesitylene Dimethylmalonic acid as some degradation. result provides insight transformation products generated during process. exhibits capacity for degradation, making promising candidate bioremediation insecticides acaricides.

Язык: Английский

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Biodegradation of dimethyl yellow by a locally isolated fungus from dye contaminated wastewater

Case Studies in Chemical and Environmental Engineering, Год журнала: 2024, Номер 9, С. 100650 - 100650

Опубликована: Фев. 2, 2024

Fungal processes for the treatment of dye contaminated wastewater have advantage being cost-effective, environmentally friendly, and producing less sludge. This research was therefore aimed at isolating characterizing fungus capable decolourizing utilizing azo (dimethyl yellow) as a sole carbon source using one-factor-at-a-time. Pour plating method used to isolate on mineral salt media (MSM) following serial dilution. The then morphologically molecularly identified Aspergillus quadrilineatus strain BUK_BCH_BTE1 with accession number OK178927 based ITS1 ITS2 rRNA gene sequence molecular phylogenetic analysis. Characterization conducted by studying effect temperature, incubation time, substrate concentration, pH, inoculum size heavy metals. decolourization growth dimethyl yellow isolated optimal concentration 200 μɡ/mL, temperature 30 °C, 100–200 μL size, pH 5.0, 72-hour time. found tolerate up 4 ppm Hg, Cu Zn, maximum inhibition shown Cr Ar. LC/MS analyses intermediate compounds produced during suggested that in fungal biodegradation detected formation yellow, 2,6-dichloro-p-benzoquinone, 1,2,3,5-tetrafluoro-4-nitrobenzene, 3,4-dinitrobenzoic acid metabolites. finding from this study is suitable candidate bioremediation coloured effluents particularly containing these compounds.

Язык: Английский

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Evaluating Potentials of Activated Carbon, Inoculum Diversity, and Total Solids Content for Improved Digestate Quality in Anaerobic Food Waste Treatment

Processes, Год журнала: 2025, Номер 13(2), С. 382 - 382

Опубликована: Янв. 30, 2025

The potential presence of toxic compounds in the digestate obtained from anaerobic digestion biodegradable waste restricts its application as a biofertilizer for soil conditioning and plant growth enhancement. aim this study was to assess quality terms nutrient composition by evaluating effects activated carbon supplementation, inoculum source, total solids content medium. food conducted over 60-day period at 25 °C 2.5 L bioreactor. results demonstrated that diversity significantly impacted composition, particularly zinc nutrient, with p-value 0.0054. This suggests microbial influences valorization organic into biofertilizer. However, on other nutrients, aside zinc, were not significant due substantial interaction effects. Furthermore, assessing impact supplementation proved challenging, it analyzed part subset two factors. analysis indicated exhibited some influence necessitating further research elucidate significance. findings may contribute enhancing

Язык: Английский

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Sustainable Waste Management as a Key Feature for Smart City

Опубликована: Фев. 16, 2025

Язык: Английский

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Numerical analysis on the thermal load and NO emission of a novel 1 MW pyrolysis gas/natural gas-fired industrial burner

Results in Engineering, Год журнала: 2025, Номер unknown, С. 104388 - 104388

Опубликована: Фев. 1, 2025

Язык: Английский

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