A DNA-Based Plasmonic Nano-Ruler DOI Open Access

Aura Cencini,

Mary Bortoluzzi,

Graziano Rilievo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2557 - 2557

Опубликована: Март 12, 2025

DNA is an exceptional building block for the fabrication of dynamic supramolecular systems with switchable geometries. Here, a self-assembled, tunable plasmonic–fluorescent nanostructure was developed. A precise sliding motion mechanism operated through control strand displacement reactions, shifting two single-strand (ssDNA) rails connected by ssDNA quasi-ring structure. The system reconfigured as nano-mechanical structure, generating six discrete configurations, and setting specific distances between tethered gold nanoparticle (AuNP) fluorophore, Sulfo-Cyanine3 (Cy3). Each configuration produced distinct fluorescence emission intensity via plasmonic quenching/enhancement effects, therefore structure behaved nano-ruler. To optimize system, reversible distance-dependent quenching or enhancement phenomena were investigated testing AuNPs diameters 5, 10, 15 nm, yielding best performances 10 nm AuNPs. Furthermore, geometric model produced, confirming observed results. fluorophore–plasmonic surface positioning, conferred ruler, led to finite state nano-machine alternative signal outputs. This mechanism, working fluorescent reporter, could find application in multiple-responsive detection nucleic acids, such viruses microRNAs.

Язык: Английский

A DNA-Based Plasmonic Nano-Ruler DOI Open Access

Aura Cencini,

Mary Bortoluzzi,

Graziano Rilievo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2557 - 2557

Опубликована: Март 12, 2025

DNA is an exceptional building block for the fabrication of dynamic supramolecular systems with switchable geometries. Here, a self-assembled, tunable plasmonic–fluorescent nanostructure was developed. A precise sliding motion mechanism operated through control strand displacement reactions, shifting two single-strand (ssDNA) rails connected by ssDNA quasi-ring structure. The system reconfigured as nano-mechanical structure, generating six discrete configurations, and setting specific distances between tethered gold nanoparticle (AuNP) fluorophore, Sulfo-Cyanine3 (Cy3). Each configuration produced distinct fluorescence emission intensity via plasmonic quenching/enhancement effects, therefore structure behaved nano-ruler. To optimize system, reversible distance-dependent quenching or enhancement phenomena were investigated testing AuNPs diameters 5, 10, 15 nm, yielding best performances 10 nm AuNPs. Furthermore, geometric model produced, confirming observed results. fluorophore–plasmonic surface positioning, conferred ruler, led to finite state nano-machine alternative signal outputs. This mechanism, working fluorescent reporter, could find application in multiple-responsive detection nucleic acids, such viruses microRNAs.

Язык: Английский

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