Metabolomic Characteristics of Aqueous Humor in Wet Age-Related Macular Degeneration and the Impact of Anti-VEGF Treatment
Investigative Ophthalmology & Visual Science,
Год журнала:
2025,
Номер
66(2), С. 37 - 37
Опубликована: Фев. 12, 2025
Purpose:
To
explore
the
alterations
in
metabolites
of
wet
age-related
macular
degeneration
(wAMD)
by
conducting
metabolomics
aqueous
humor
from
patients
with
wAMD
and
to
assess
potential
effects
anti-vascular
endothelial
growth
factor
(anti-VEGF)
on
these
metabolites.
Methods:
Metabolomic
analysis
was
performed
30
receiving
anti-VEGF
treatments
20
controls,
via
ultra-high
performance
liquid
chromatography
tandem
mass
spectrometry.
The
samples
collected
untreated
were
classified
as
pre-wAMD
group.
Accordingly,
one
treatment
designated
post-wAMD
Individuals
further
into
responders
nonresponders
according
their
reaction
treatment.
Principal
component
analysis,
hierarchical
cluster
Kyoto
Encyclopedia
Genes
Genomes
annotation
enrichment
subsequently
performed.
Machine
learning
receiver
operating
characteristic
curve
analyses
used
analyze
vital
Results:
Among
1001
verified
humor,
306
compounds
separated
control
group,
whereas
68
differentiated
pre-wAMD.
Enrichment
metabolic
pathways
noted
ABC
transporters,
thiamine
metabolism,
glycerophospholipid
mammalian
target
rapamycin
signaling
pathway
tyrosine
so
on.
curves
suggested
that
δ-valerolactam
could
not
only
distinguish
between
but
also
differentiate
Changes
acylcarnitine
observed
wAMD.
Conclusions:
There
noticeable
involving
many
are
associated
pathway.
It
is
possible
can
be
applied
a
biomarker
Язык: Английский
Bioactive lipid signaling and lipidomics in macrophage polarization: Impact on inflammation and immune regulation
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 14, 2025
Macrophages,
crucial
innate
immune
cells,
defend
against
pathogens
and
resolve
inflammation,
maintaining
tissue
balance.
They
perform
phagocytosis,
present
antigens
to
T
bond
adaptive
immunity
through
various
activation
states.
Classical
is
associated
with
Th1
responses
interferon
γ
production,
while
alternative
activation,
induced
by
interleukin
4,
characterized
increased
endocytosis,
reduced
secretion
of
pro-inflammatory
cytokines,
roles
in
immunoregulation
remodeling.
Although
these
represent
opposite
extremes
observed
vitro
,
the
remarkable
plasticity
macrophages
allows
for
a
wide
spectrum
phenotypes
that
are
complex
characterize
experimentally.
While
application
omics
techniques
has
resulted
significant
advances
characterization
macrophage
polarization,
lipidomic
studies
have
received
lesser
attention.
Beyond
their
role
as
structural
components
energy
sources,
lipids
function
signaling
molecules
regulate
thereby
shaping
responses.
This
work
reviews
interaction
between
lipid
exploring
how
metabolism
influences
phenotype
function.
These
insights
offer
potential
therapeutic
strategies
immune-mediated
diseases
inflammation-related
disorders,
including
inflammaging.
Язык: Английский
The Interplay Between Immunity, Inflammation and Endothelial Dysfunction
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(4), С. 1708 - 1708
Опубликована: Фев. 17, 2025
The
endothelium
is
pivotal
in
multiple
physiological
processes,
such
as
maintaining
vascular
homeostasis,
metabolism,
platelet
function,
and
oxidative
stress.
Emerging
evidence
the
past
decade
highlighted
immunomodulatory
function
of
endothelium,
serving
a
link
between
innate,
adaptive
immunity
inflammation.
This
review
examines
regulation
immune-inflammatory
axis
by
discusses
immune
functions,
explores
pathophysiological
processes
leading
to
endothelial
dysfunction
various
metabolic
disturbances,
including
hyperglycemia,
obesity,
hypertension,
dyslipidaemia.
final
section
focuses
on
novel,
repurposed,
emerging
therapeutic
targets
that
address
dysfunction.
Язык: Английский
Changes in feeding behavior, milk yield, serum indexes, and metabolites of dairy cows in three weeks postpartum
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 7, 2025
Parturition
and
lactation
stress
greatly
affect
physiological
metabolic
status
of
postpartum
cows.
Monitoring
feeding
behavior
can
help
assess
the
health
This
study
aimed
to
explore
changes
in
behavior,
milk
yield
(MY),
serum
indexes,
metabolites
dairy
cows
during
three
weeks
postpartum.
Furthermore,
relationships
between
were
investigated.
One
hundred
seventy-eight
healthy
multiparous
Holstein
enrolled
continuously
record
variables,
including
time
(FT),
frequency
(FF),
average
(AFT),
using
Nedap
neck
collar
devices,
wherein
was
recorded.
Out
178
cows,
20
with
same
parity
number
(2
parities),
body
condition
score
(3.0
±
0.25,
recorded
7
days
before
parturition),
eutocic
calvings,
selected
indexes
on
week
1,
2,
3
Then,
further
based
similar
calving
date
(±
d)
for
transition
detection.
Compared
1
postpartum,
FT
AFT
normalized
values
2
significantly
increased
(P
<
0.001).
The
positively
correlated
MY
0.01).
total
protein
(TP),
cholesterol
(T-Chol)
IgA
contents
compared
while
β-hydroxybutyric
acid
(BHBA)
concentration
decreased.
Serum
malondialdehyde
(MDA),
interleukin-6
(IL-6),
leptin
(LEP)
2nd
declined
1st
negatively
BHBA
level,
TP
T-Chol
0.05).
According
UPLC-MS/MS
pathway
analysis
results,
levels
glycerophospholipids
changed
most
first
which
mainly
related
glycerophospholipid
metabolism.
Finally,
correlation
showed
that
29
(GPs)
fatty
acyls
(FAs)
Together,
findings
suggest
such
as
AFT,
could
serve
reliable
indicators
energy
metabolism
Язык: Английский
Characterization and Comparison of Lipids in Yak Colostrum, Buffalo Colostrum, and Cow Colostrum Based on UHPLC-QTOF-MS Lipidomics
Dairy,
Год журнала:
2025,
Номер
6(2), С. 14 - 14
Опубликована: Март 27, 2025
Colostrum
is
a
nutrient-dense
food
rich
in
proteins,
immune
modulators,
and
growth
factors
essential
for
neonatal
development.
Its
lipids
serve
as
key
energy
source
facilitate
cellular
functions.
While
yak
colostrum
(YC)
buffalo
(BC)
exhibit
high
nutritional
value,
their
lipid
compositions
remain
unclear.
This
study
employed
lipidomics
to
analyze
compare
the
profiles
of
YC
BC
with
cow
(CC),
identifying
872
across
33
subclasses.
Differential
analysis
revealed
137,
100,
131
lipids,
significant
expression
differences
between
vs.
CC,
BC,
respectively.
exhibited
higher
levels
α-linolenic
acid
docosahexaenoic
acid,
suggesting
potential
role
enhancing
neurodevelopment
cognitive
function.
In
contrast,
upregulation
specific
components
such
phosphatidylethanolamine
(PE)
phosphatidylinositol
(PI),
coupled
downregulation
lysophosphatidylcholine
(LPC)
lysophosphatidylethanolamine
(LPE),
indicated
benefit
metabolism
inflammatory
regulation.
These
findings
suggest
that
may
be
particularly
suitable
neonates
requiring
enhanced
support,
while
offer
advantages
modulation.
The
provides
critical
insights
into
distinct
laying
scientific
foundation
development
tailored
supplements.
results
also
hold
implications
dairy
industry,
driving
innovation
optimization
colostrum-based
products
meet
diverse
demands.
Язык: Английский
Advancing the Biochemical Understanding of Maple Syrup Urine Disease and the Impact of Liver Transplantation: A Pilot Study
Journal of Proteome Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
Maple
syrup
urine
disease
(MSUD)
is
a
rare
autosomal
recessive
metabolic
disorder
causing
impaired
branched-chain
amino
acid
(BCAA)
catabolism
and
systemic
dysregulation.
MSUD
has
an
incidence
of
approximately
1
in
185,000
U.S.
births,
with
much
higher
prevalence
the
Mennonite
communities
(up
to
400
live
births
due
c.1312T
>
A
p.Tyr438Asn
BCKDHA
founder
mutation).
Using
multiomic
approach
integrating
metabolomics,
lipidomics,
proteomics,
we
analyzed
blood
samples
from
three
patients
on
BCAA-restricted
diet
(MSUDDR),
two
who
received
liver
transplants
(MSUDLT),
six
healthy
controls.
Gene
ontology
analysis
integrated
omics
data
confirmed
imbalances
MSUD,
highlighting
increases
glycolysis,
oxidative
phosphorylation,
purine
metabolism.
Lipidomic
revealed
disruptions
sphingolipids
lysophosphatidylcholines,
affecting
signaling
membrane
integrity.
Liver
transplantation
corrected
some
abnormalities,
but
key
metabolites
proteins
remained
altered.
Proteomic
significant
alterations
redox
homeostasis,
energy
metabolism,
cytoskeletal
organization
partial
recovery
post-transplantation.
Post-translational
modifications
indicated
ongoing
stress
immune
activation
MSUDLT
group.
Elevated
levels
l-isoleucine,
l-valine,
their
ketoacids
persisted
post-transplant,
correlating
lipid
remodeling,
protein
folding.
These
findings
provide
insights
into
MSUD-associated
dysfunction
highlight
potential
therapeutic
targets.
Язык: Английский