Journal of Inflammation Research,
Год журнала:
2025,
Номер
Volume 18, С. 6529 - 6541
Опубликована: Май 1, 2025
Rheumatoid
arthritis
(RA)
is
a
chronic
inflammatory
autoimmune
disease
that
can
lead
to
joint
deformities,
functional
loss,
and
significant
reduction
in
patients'
quality
of
life.
It
also
imposes
considerable
medical
socio-economic
burden.
Iron-induced
cell
death,
or
ferroptosis,
unique
form
programmed
death
characterized
by
dysregulated
iron
metabolism
the
accumulation
lipid
peroxides
resulting
from
increased
reactive
oxygen
species
(ROS)
reduced
activity
glutathione
peroxidase
4
(GPX4).
The
cause
cellular
damage,
promotes
responses
destruction.
This
process
not
only
plays
crucial
role
pathogenesis
RA,
but
provides
new
therapeutic
targets
for
its
treatment.
In
this
review,
we
summarize
regulatory
mechanisms
ferroptosis
RA.
These
include
roles
regulating
oxidative
stress
peroxidation,
inhibiting
abnormal
proliferation
synovial
fibroblasts
(FLSs),
preventing
cartilage
erosion,
restoring
immune
homeostasis
responses,
other
aspects.
Finally,
discuss
potential
clinical
applications,
future
prospects
ferroptosis-based
therapies
RA
Background:
The
research
focused
on
the
effects
of
pseudorabies
virus
gene
expression
in
piglet
brain
tissue.
Objectives:
goal
was
to
understand
changes
brains
due
infection.
Design:
study
used
a
comparative
approach
with
infected
and
control
groups.
Methods:
Twelve
2-month-old
piglets
were
divided
into
virus-infected
PBS-treated
groups,
tissue
analyzed
after
7
days.
Results:
Infected
showed
increased
oligodendrocyte
counts
virus-positive
signals.
Transcriptomic
analysis
revealed
269
differentially
expressed
genes,
149
up-regulated
120
down-regulated.
Gene
ontology
Kyoto
Encyclopedia
Genes
Genomes
analyses
indicated
these
genes
are
involved
signal
transduction,
transmembrane
transport,
apoptosis,
neuroactive
ligand–receptor
interaction.
Quantitative
fluorescent
PCR
validated
findings,
particularly
for
related
pathways,
ferroptosis,
IL-17
signaling.
Conclusion:
provides
valuable
insights
molecular
alterations
caused
by
tissue,
enhancing
our
understanding
virus’s
pathogenic
mechanisms.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 17, 2025
Systemic
lupus
erythematosus
(SLE)
is
a
complex
autoimmune
disease
with
heterogeneous
clinical
manifestations.
Understanding
the
molecular
mechanisms
of
SLE
crucial
for
developing
effective
therapeutic
strategies.
This
study
downloaded
microarray
datasets
from
Gene
Expression
Omnibus
(GEO)
database.
Single-cell
RNA
sequencing
(scRNA-seq)
data
was
processed
to
identify
19
clusters
and
annotated
five
major
cell
types.
Then
we
calculated
mitochondrial-related
genes
(MRGs)
ferroptosis-related
(FRGs)
scores.
FRGs
scored
highest
in
Megakaryocytes,
while
MRGs
B
cells.
By
employing
pseudotime
analysis,
cell-cell
communication
Single-Cell
Regulatory
Network
Inference
Clustering
(SCENIC)
explored
heterogeneity
cells
SLE.
Hub
were
identified
using
high-dimensional
weighted
correlation
network
analysis
(hdWGNCA)
machine
learning
algorithms,
leading
development
predictive
diagnostic
model
high
accuracy.
Immune
infiltration
revealed
significant
correlations
between
biomarkers
various
immune
Lastly,
docking
studies
suggested
Doxorubicin
may
exert
effects
by
affecting
these
biomarkers.
offers
new
insights
into
pathogenesis
provide
valuable
directions
future
research.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 2, 2025
Ferroptosis,
a
recently
proposed
form
of
regulated
cell
death,
is
characterized
by
surge
in
reactive
oxygen
species
and
subsequent
depletion
glutathione.
The
mitochondria
nucleoli
play
pivotal
roles
the
process
ferroptosis.
Therefore,
monitoring
interactions
between
during
ferroptosis
crucial
for
clarifying
its
physiological
pathological
processes.
In
this
study,
we
designed
synthesized
near-infrared
fluorescence
probe
MINU,
which
exhibits
excellent
stability
against
biological
ions
pH
environments.
Due
to
cationic
structure
good
DNA
affinity,
MINU
can
target
both
nucleoli.
Cell
imaging
demonstrates
that
reversibly
migrate
response
changes
mitochondrial
membrane
potential.
By
detecting
localization
intensity
signals,
effectively
distinguish
normal
cell,
apoptotic
ferroptotic
cell.
Monitoring
allows
us
more
accurately
appreciate
processes
