Interactomic exploration of LRRC8A in volume-regulated anion channels
Cell Death Discovery,
Год журнала:
2024,
Номер
10(1)
Опубликована: Июнь 22, 2024
Abstract
Ion
channels
are
critical
in
enabling
ion
movement
into
and
within
cells
important
targets
for
pharmacological
interventions
different
human
diseases.
In
addition
to
their
transport
abilities,
interact
with
signalling
scaffolding
proteins,
which
affects
function,
cellular
positioning,
links
intracellular
pathways.
The
study
of
“channelosomes”
has
the
potential
uncover
involvement
diseases,
although
this
field
research
is
still
emerging.
LRRC8A
gene
that
encodes
a
crucial
protein
involved
formation
volume-regulated
anion
(VRACs).
Some
studies
suggest
could
be
valuable
prognostic
tool
types
cancer,
serving
as
biomarker
predicting
patients’
outcomes.
expression
levels
might
linked
tumour
progression,
metastasis,
treatment
response,
its
implications
cancer
can
varied.
Here,
publicly
accessible
databases
patients
were
systematically
analysed
determine
if
correlation
between
VRAC
channel
survival
rate
exists
across
distinct
types.
Moreover,
we
re-evaluated
impact
on
proliferation
migration
colon
via
HCT116
LRRC8A-KO
cells,
current
topic
debate
literature.
addition,
investigate
role
signalling,
conducted
biotin
proximity-dependent
identification
(BioID)
analysis,
revealing
cell-cell
junctions,
mechanisms
govern
calcium
homeostasis,
kinases,
GTPase
signalling.
Overall,
dataset
improves
our
understanding
LRRC8A/VRAC
explores
new
avenues
while
identifying
promising
therapeutic
promoting
inventive
methods
disease
treatment.
Язык: Английский
Volume-regulated anion channels conduct ATP in undifferentiated mammary cells and promote tumorigenesis in xenograft nude mouse
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 15, 2025
The
high
interstitial
ATP
concentration
in
the
cancer
microenvironment
is
a
major
source
of
adenosine,
which
acts
as
strong
immune
suppressor.
However,
release
has
not
been
elucidated.
We
measured
during
hypotonic
stress
using
real-time
luminescence
imaging
system
breast
cell
lines
and
primary
cultured
mammary
cells.
In
lines,
was
released
with
slowly
rising
diffuse
pattern,
whereas
cells,
intermittently
transient-sharp
peaks.
pattern
changed
to
by
cholera
toxin
treatment
reverse
change
induced
transforming
growth
factor
(TGF)
β
treatment.
DCPIB,
an
inhibitor
volume-regulated
anion
channels
(VRACs),
suppressed
pattern.
inflammatory
mediator
sphingosine-1-phosphate
(S1P)
isovolumetrically.
Knockdown
A
isoform
leucine-rich
repeat-containing
protein
8
(LRRC8A),
essential
molecular
entity
VRACs,
shRNA
nude
mouse
xenograft
model,
LRRC8A
knockdown
tumorigenesis
subcutaneously
implanted
These
results
suggest
that
abundantly
expressed
VRACs
are
conduit
undifferentiated
including
Язык: Английский
Recent insights on the impact of SWELL1 on metabolic syndromes
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 21, 2025
SWELL1
is
a
key
component
of
the
volume-regulated
anion
channel
(VRAC)
and
participates
in
cell
volume
regulation
as
an
ion
plasma
membrane
protein.
While
early
studies
focused
on
its
role
immune
development
tumor
progression,
recent
have
revealed
that
plays
important
metabolic
diseases.
Studies
shown
extensively
involved
physiological
processes
peripheral
tissues,
including
adipocyte
hypertrophy,
skeletal
muscle
regulation,
insulin
secretion,
hepatic
lipid
metabolism
through
interactions
with
signaling
pathway.
These
functions
play
roles
pathogenesis
obesity,
type
2
diabetes
mellitus
(T2DM),
non-alcoholic
fatty
liver
disease
(NAFLD),
suggesting
may
be
new
target
for
treatment
In
this
review,
we
focus
structural
functional
characteristics
to
provide
in-depth
explanation
syndrome,
especially
pathway,
basis
therapeutic
strategies
diseases
targeting
SWELL1.
Язык: Английский
Phosphoinositide kinases in cancer: from molecular mechanisms to therapeutic opportunities
Nature reviews. Cancer,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 3, 2025
Язык: Английский
In silico pan-cancer analysis of VRAC subunits and their prognostic roles in human cancers
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 11, 2025
Язык: Английский
Identification of vitamin D-related signature for predicting the clinical outcome and immunotherapy response in hepatocellular carcinoma
Medicine,
Год журнала:
2024,
Номер
103(19), С. e37998 - e37998
Опубликована: Май 10, 2024
Hepatocellular
carcinoma
(HCC)
is
one
of
the
most
common
cancers
globally,
seriously
endangering
people
health.
Vitamin
D
was
significantly
associated
with
tumor
progression
and
patients’
prognosis.
Integrative
10
machine
learning
algorithms
were
used
to
develop
a
D-related
signature
(VRS)
training
cohort
3
testing
cohorts.
The
performance
VRS
in
predicting
immunology
response
verified
using
several
approaches.
optimal
constructed
by
stepCox
+
superPC
algorithm.
acted
as
risk
factor
for
HCC
patients.
patients
high-risk
score
had
poor
clinical
outcome
AUCs
1-,
3-,
5-year
ROC
0.786,
0.755,
respectively.
A
higher
level
CD8
cytotoxic
T
cells
B
obtained
low-risk
score.
There
PD1&CTLA4
immunophenoscore
TMB
Lower
TIDE
escape
found
cases
IC50
value
camptothecin,
docetaxel,
crizotinib,
dasatinib,
erlotinib
lower
cancer-related
hallmarks,
including
angiogenesis,
glycolysis,
NOTCH
signaling.
Our
study
proposed
novel
HCC,
which
served
an
indicator
immunotherapy
responses
HCC.
Язык: Английский
A comprehensive investigation of PRMT5 in the prognosis and ion channel features of lung cancer
Frontiers in Oncology,
Год журнала:
2024,
Номер
14
Опубликована: Ноя. 29, 2024
The
increasing
incidence
and
mortality
associated
with
lung
cancer
(LC)
is
a
significant
global
health
challenge.
underlying
mechanisms
contributing
to
LC
remain
inadequately
understood.
However,
emerging
evidence
suggests
that
the
epigenetic
modifier
protein
arginine
methyltransferase
5
(PRMT5)
plays
complex
role
in
various
cellular
processes,
including
DNA
repair,
gene
transcription,
alternative
splicing,
through
its
function
catalyzing
symmetric
dimethylation
of
both
histone
non-histone
proteins.
In
this
study,
we
examined
functional
PRMT5
utilizing
LC-related
datasets
(GSE30219,
GSE50081,
TCGA
cohort)
series
analyses.
Our
findings
revealed
was
significantly
overexpressed
samples
compared
normal
tissues
correlated
overall
survival
disease-free
rates.
Additionally,
PRDM1
identified
as
key
exhibiting
strong
interaction
PRMT5.
prognostic
model
integrated
clinical
factors
demonstrated
robust
performance
assessing
outcomes.
Elevated
levels
were
poor
prognosis
LC,
evidenced
by
analyses
GSE30219,
TCGA-LC
datasets.
Furthermore,
27
ion
channel
(IC)
genes
exhibited
correlation
adenocarcinoma
(LUAD),
which
9
statistically
KM
analysis.
Strikingly,
all
genes,
LRRC8A,
same
PRMT5,
LUAD.
research
highlights
potential
novel
biomarker
relationship
IC
LC.
Язык: Английский
The Druggable Target Potential of NF-κB-Inducing Kinase (NIK) in Cancer
International Journal of Translational Medicine,
Год журнала:
2024,
Номер
5(1), С. 1 - 1
Опубликована: Дек. 25, 2024
NF-κB-inducing
kinase
(NIK)
is
primarily
recognized
for
its
role
as
the
apical
that
activates
non-canonical
NF-κB
signaling
and
involvement
in
immune
system
regulation.
NIK
crucial
maintaining
cellular
health
by
regulating
fundamental
processes
such
differentiation,
growth,
survival.
Emerging
evidence
suggests
dysregulated
expression
or
function
of
non-lymphoid
cells
a
key
factor
cancer
progression.
While
deficiency
causes
severe
dysfunction,
overexpression
excessive
activation
linked
to
inflammatory
diseases,
metabolic
disorders,
development.
The
development
small
molecule
inhibitors
targeting
has
sparked
optimism
clinical
intervention,
positioning
promising
druggable
mediator
cancer.
ongoing
progress
creating
novel
offers
new
opportunities
testing
NIK-targeted
therapies,
potentially
advancing
application
NIK-based
treatments.
Язык: Английский