Lipid specificity of action of SARS-CoV-2 fusion peptide fragments on model membranes DOI Creative Commons
Egor V. Shekunov, Pavel E. Volynsky, Svetlana S. Efimova

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Abstract The study focuses on investigating the interaction of SARS-CoV-2 fusion peptide fragment with model membranes various lipid composition to elucidate molecular mechanisms peptide-derived membrane fusion. work utilized short which is homologous 816-827 region native FP (FP ) and contains highly conserved LLF motif responsible for fusion, its ineffective analogue (mFP ), where was replaced AAA. Using fluorescence assay, it demonstrated that plays a key role in inducing liposome whereas replacement completely abolishes this capability. fusogenic activity strictly depended vesicle composition. It potentiated by phosphatidylethanolamine inhibited phosphatidylserine. Molecular dynamics revealed both peptides predominantly adopt an α-helical conformation; however, interacts more strongly hydrophobic core increasing peptide-lipid contacts, while mutant version exhibits superficial localization. Differential scanning microcalorimetry data indicated ability 816- 827 disturb packing increased decreasing tail length. underlying were identified, specifically cluster phospholipid head groups own vicinity. As result, local regions positive spontaneous curvature are formed outer monolayer, facilitating findings highlight architecture mechanism viral host cells.

Язык: Английский

Virally-initiated pain states: phenotype, mechanisms, and future directions DOI Creative Commons
Sara A. Dochnal, Steven P. Cohen, Mark R. Hutchinson

и другие.

Frontiers in Pain Research, Год журнала: 2025, Номер 6

Опубликована: Янв. 28, 2025

The recent SARS-CoV-2 pandemic has underscored the significance of viral infections, affecting billions lives and costing trillions dollars globally. Even beyond SARS-CoV-2, common infections with viruses like influenza, HIV, herpesviruses have profound impacts their typical manifestations, often triggering acute chronic pain syndromes that can be life-altering. These virally induced states arise through direct replication within neurons, or indirectly, via immune responses to infection in both contexts afferent signaling dorsal root ganglion (DRG) subsequent higher order integration intracranial systems. Varicella-zoster virus (VZV), influenza virus, each provide a unique lens which examine interplay between activity pain. This perspective paper is not meant an exhaustive review virally-induced neuropathic states. It seeks explore curated aspects complexities these states, identify research gaps, suggest solutions using nanoscale molecular understanding psychoneuroimmunological biopsychosocial frameworks. Each subheading accompanied by list related issues for study we think will lead advances our vexing phenotype associated infection.

Язык: Английский

Процитировано

0
Treatment of COVID‐19‐Associated Ageusia and Xerostomia DOI
Hironori Tsuchiya

Oral Diseases, Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

Язык: Английский

Процитировано

0
Ectonucleotidases DOI
Raíssa Leite-Aguiar, Marco De Giorgi, Luiz Eduardo Baggio Savio

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Процитировано

0
Mechanisms and Interventions of Aeromonas Hydrophila-Induced Hepatic Injury in Megalobrama Amblycephala: Focus on Ros-Mediated Mitochondrial Apoptosis DOI

Zhensheng Wang,

Wenya Zhai,

Canxun Ye

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Serum lipidome reveals lipid metabolic dysregulation in severe fever with thrombocytopenia syndrome DOI Creative Commons
Shuai Guo, Yunjun Yan, Jingyao Zhang

и другие.

BMC Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 14, 2024

Severe fever with thrombocytopenia syndrome (SFTS) is a rapidly progressing infectious disease high fatality rate caused by novel bunyavirus (SFTSV). The role of lipids in viral infections well-documented; however, the specific alterations lipid metabolism during SFTSV infection remain elusive. This study aims to elucidate metabolic dysregulations early stages SFTS patients. prospectively collected peripheral blood sera from 11 critical patients, 37 mild and 23 healthy controls for lipidomics analysis. A systematic bioinformatics analysis was conducted three aspects integrating differential expressions, correlations, lipid-clinical indices correlations reveal serum dysregulation SFTSV-infected individuals. Our findings significant Specifically, compared controls, patients exhibited distinct modes expression: increased levels including phosphatidylserine (PS), hexosylceramide (HexCer), triglycerides (TG); decreased lysophosphatidylcholine (LPC), acylcarnitine (AcCa), cholesterol esters (ChE); showing "dual changes" phosphatidylcholine (PC) phosphatidylethanolamine (PE). Finally, based on pathways literature analysis, we systematically elucidated potential mechanisms underlying stage infection. presents first global lipidome profile reveals patterns These provide new basis diagnosis, treatment, further investigation disease.

Язык: Английский

Процитировано

2
Research Advances on the Role of Lipids in the Life Cycle of Human Coronaviruses DOI Creative Commons
Cuiling Ding, Yibo Chen,

Miao Gen

и другие.

Microorganisms, Год журнала: 2023, Номер 12(1), С. 63 - 63

Опубликована: Дек. 28, 2023

Coronaviruses (CoVs) are emerging pathogens with a significant potential to cause life-threatening harm human health. Since the beginning of 21st century, three highly pathogenic and transmissible CoVs have emerged, triggering epidemics posing major threats global public enveloped viruses encased in lipid bilayer. As fundamental components cells, lipids can play an integral role many physiological processes, which been reported important roles life cycle CoVs, including viral entry, uncoating, replication, assembly, release. Therefore, research on CoV provide basis for better understanding infection mechanism targets development new antiviral strategies. In this review, advances different stages possible that interfere discussed.

Язык: Английский

Процитировано

5
Sequestration of membrane cholesterol by cholesterol-binding proteins inhibits SARS-CoV-2 entry into Vero E6 cells DOI Creative Commons
Magdalena Kulma, Aleksandra Šakanović, Apolonija Bedina-Zavec

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 716, С. 149954 - 149954

Опубликована: Апрель 16, 2024

Membrane lipids and proteins form dynamic domains crucial for physiological pathophysiological processes, including viral infection. Many plasma membrane proteins, residing within enriched with cholesterol (CHOL) sphingomyelin (SM), serve as receptors attachment entry of viruses into the host cell. Among these, human coronaviruses, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), use associated initial binding internalization. We hypothesized that interaction lipid-binding CHOL in could sequestrate thus affect efficiency virus cells, preventing steps have prepared CHOL-binding high affinities mammalian cells. Binding perfringolysin O domain four (D4) its variant D4E458L to impaired internalization receptor-binding SARS-CoV-2 spike protein pseudovirus complemented protein. replication Vero E6 cells was also decreased. Overall, our results demonstrate integrity CHOL-rich accessibility play an essential role cell entry.

Язык: Английский

Процитировано

1
Preliminary Study on the Restoration of the Phospholipid Profile in Serum from Patients with COVID-19 by Treatment with Vitamin E DOI Open Access
Marí­a Elena Soto, Linaloe Manzano-Pech, Verónica Guarner‐Lans

и другие.

Опубликована: Июнь 10, 2024

SARS-CoV-2 is an obligatory intracellular pathogen that requires of a lipid bilayer membrane for its transport, to build nucleocapsid envelope and fuse with the host cell. The biological membranes are constituted by phospholipids (PLs) vitamin E (Vit E) protects them from oxidative stress (OS). aim this study was demonstrate if treatment Vit restores modified profile FA in PLs serum patients coronavirus disease-19 (COVID-19). We evaluated E, total fatty acids (TFA), acid (FAPL), (TPLs), 8-isoprostane, thromboxane B2 (TXB2), prostaglandins (PGE2 6-keto-PGF1α), interleukin-6 (IL-6) C-reactive protein (CRP) 22 COVID-19 before after compared values those 23 healthy subjects (HS). showed decrease TPLs, FAPL TFA comparison HS (p≤0.01) restored their levels (p≤0.04). Likewise, there increase IL-6 CRP (p≤0.001) decreased (p≤0.001). Treatment contributes restore infection leads OS inflammatory process.

Язык: Английский

Процитировано

1
Lower serum LDL-C levels are associated with poor prognosis in severe fever with thrombocytopenia syndrome: a single-center retrospective cohort study DOI Creative Commons
Shuai Guo, Qing Dong,

Zhang Mao-mei

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Июнь 24, 2024

Background Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease triggered by a novel bunyavirus (SFTSV). Characterized fever, thrombocytopenia, leukocytopenia, and multiple organ dysfunction manifestations, its primary mode of transmission through tick bites. Despite the critical role lipid metabolism in viral infections, lipids SFTS remains unclear. Methods This retrospective study analyzed 602 patients treated at Shandong Public Health Clinical Center from January 2021 to December 2023. Based on endpoint events, were classified into survival (S) death (D) groups. The S group was further non-critical (non-C) (C) groups based symptoms. All followed up for least 28 days after admission. Propensity score matching, multivariable logistic regression, analysis, time trend mediation analysis conducted assess association between LDL-C levels prognosis SFTS. Results serum admission significantly lower D C than non-C regression models indicated potential poor restricted cubic spline showed unidirectional mortality, cutoff value 1.59 mmol/L. revealed higher earlier mortality low-LDL-C high-LDL-C group. trends over post-admission that gradually increased SFTS, favorable prognosis. Finally, low are associated hepatic, cardiac, coagulation functions. Conclusion Low potentially

Язык: Английский

Процитировано

1
Increased viperin expression induced by avian infectious bronchitis virus inhibits viral replication by restricting cholesterol synthesis: an in vitro study DOI Creative Commons
Yu Zhang,

T Zhang,

Yan-Peng Lu

и другие.

Veterinary Research, Год журнала: 2024, Номер 55(1)

Опубликована: Сен. 27, 2024

Язык: Английский

Процитировано

1