Microorganisms,
Год журнала:
2024,
Номер
12(12), С. 2532 - 2532
Опубликована: Дек. 8, 2024
Enterotoxigenic
Escherichia
coli
(ETEC)
is
a
major
pathogen
causing
diarrhea
in
humans
and
animals,
with
increasing
antimicrobial
resistance
posing
growing
challenge
recent
years.
Lytic
bacteriophages
(phages)
offer
targeted
environmentally
sustainable
approach
to
combating
bacterial
infections,
particularly
eliminating
drug-resistant
strains.
In
this
study,
ETEC
strains
were
utilized
as
indicators,
stable,
high-efficiency
phage,
designated
vB_EcoM_JE01
(JE01),
was
isolated
from
pig
farm
manure.
The
genome
of
JE01
dsDNA
molecule,
measuring
168.9
kb,
transmission
electron
microscope
revealed
its
characteristic
T4-like
Myoviridae
morphology.
effectively
lysed
multi-drug-resistant
isolates.
Stability
assays
demonstrated
that
retained
activity
across
temperature
range
20
°C
50
pH
3–11,
showing
resilience
ultraviolet
radiation
chloroform
exposure.
Furthermore,
suppressed
adhesion
porcine
intestinal
epithelial
cells
(IPEC-J2),
mitigating
the
inflammatory
response
triggered
by
ETEC.
To
investigate
vivo
antibacterial
efficacy
phage
preparations,
model
established
using
germ-free
mice
infected
strain.
findings
indicated
12
h
post-ETEC
inoculation,
intragastric
administration
significantly
reduced
mortality,
alleviated
gastrointestinal
lesions,
decreased
colonization
jejunum,
expression
cytokines
IL-6
IL-8.
These
results
demonstrate
therapeutic
benefit
treating
ETEC-induced
mice.
Additionally,
fluorescent
incorporating
red
protein
(RFP)
engineered,
pharmacokinetics
therapy
preliminarily
assessed
through
fluorescence
imaging
showed
localized
jejunum
rapidly,
within
45
min.
Moreover,
markedly
slowed
presence
target
gut,
suggesting
sustained
bacteriolytic
ETEC-infected
intestine.
conclusion,
study
establishes
foundation
for
development
phage-based
therapies
against
Frontiers in Veterinary Science,
Год журнала:
2024,
Номер
11
Опубликована: Авг. 7, 2024
Oxidative
damage
resulting
from
weaning
stress
significantly
impacts
the
growth
performance
and
health
status
of
piglets.
Taurine,
a
dietary
antioxidant
with
diverse
functions,
was
investigated
in
this
study
for
its
protective
role
against
stress-induced
oxidative
underlying
mechanism.
Forty
28-day-old
male
castrated
weaned
piglets
were
randomly
assigned
to
four
groups.
The
control
group
received
basal
diet,
while
experimental
groups
fed
diet
supplemented
0.1,
0.2%,
or
0.3%
taurine
over
28-day
period.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 16, 2024
Introduction
Enterotoxigenic
Escherichia
coli
(ETEC)
is
the
main
diarrhea-causing
pathogen
in
children
and
young
animals
has
become
a
global
health
concern.
Berberine
type
of
“medicine
food
homology”
long
history
use
China,
particularly
treating
gastrointestinal
disorders
bacterial
diarrhea.
Methods
In
this
study,
we
explored
effects
berberine
on
growth
performance,
intestinal
inflammation,
oxidative
damage,
microbiota
weaned
piglet
model
ETEC
infection.
Twenty-four
piglets
were
randomly
divided
into
four
groups—a
control
group
(fed
basal
diet
[BD]
infused
with
saline),
BD+ETEC
ETEC),
LB+ETEC
0.05%
infection),
HB+ETEC
0.1%
ETEC).
Results
significantly
improved
final
body
weight
(BW),
average
daily
gain
(ADG),
feed
intake
(ADFI)
(
P
<0.05)
piglets,
effectively
decreased
incidence
diarrhea
among
<0.05).
Additionally,
downregulated
expression
levels
genes
encoding
TNF-α,
IL-1β,
IL-6,
IL-8,
TLR4,
MyD88,
NF-κB,
IKKα,
IKKβ
small
intestine
infection
upregulated
coding
for
Nrf2,
CAT,
SOD1,
GPX1,
GST,
NQO1,
HO-1,
GCLC,
GCLM
12
functional
COG
categories
7
KEGG
signaling
pathways.
A
correlation
analysis
showed
that
increased
relative
abundance
beneficial
bacteria
(Gemmiger,
Pediococcus,
Levilactobacillus,
Clostridium,
Lactiplantibacillus,
Weissella,
Enterococcus,
Blautia,
Butyricicoccus)
pathogenic
(Prevotella,
Streptococcus,
Parabacteroides,
Flavonifractor,
Alloprevotella)
known
to
be
closely
related
inflammation
stress
piglets.
conclusion,
disrupted
upregulating
TLR4/MyD88/NF-κB
Nrf2
pathways,
consequently
leading
stress-induced
damage.
Discussion
Our
data
indicated
can
optimize
balance
modulate
thus
helping
alleviate
damage
caused
by
Veterinary Quarterly,
Год журнала:
2025,
Номер
45(1), С. 1 - 9
Опубликована: Янв. 1, 2025
Weaning
stress-induced
diarrhea
is
a
serious
issue
in
pig
production.
This
study
aimed
to
evaluate
the
potential
of
glucose
oxidase
(GOD)
as
an
antibiotic
substitute
alleviate
and
improve
gut
health
weaned
piglets.
According
randomized
complete
block
design,
250
piglets
around
21
d
age
were
allocated
into
5
groups
(5
replicates/group),
which
received
basal
diet
without
or
with
supplemental
200
mg/kg
antibiotic,
500,
1000
2000
U/kg
GOD,
respectively.
Dietary
treatments
did
not
affect
(p
>
0.05)
growth
performance
However,
all
doses
GOD
equivalent
superior
reduce
<
well
increase
thymus
index,
hepatic
colonic
antioxidant
properties.
addition
at
reduced
cecal
rectal
pH
value,
They
also
displayed
similar
efficacy
improving
duodenal
jejunal
morphology
along
certain
tight
junction
proteins
expression
jejunum
colon.
Collectively,
represents
alternative
through
associating
ameliorations
intestinal
structure
functions.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 10, 2025
Abstract
Enterotoxigenic
Escherichia
coli
(ETEC)
is
a
common
cause
of
diarrhea
in
human
and
animals,
including
pigs.
Enterotoxins
are
important
virulence
factors
for
ETEC.
Although
lot
known
on
the
mechanism
enterotoxin-induced
diarrhea,
less
about
their
effects
innate
immune
cells
like
monocytes.
Monocytes
can
differentiate
into
macrophages
dendritic
play
pivotal
role
as
bridge
between
adaptive
system.
Understanding
interaction
ETEC
enterotoxins
monocytes
help
development
more
effective
preventive
therapeutic
strategies
to
combat
this
disease.
In
study,
we
aimed
investigate
heat
labile
enterotoxin
(LT)
stable
(STa)
produced
by
porcine
Our
results
show
that
STa
did
not
affect
cell
viability
effector
functions
LT,
other
hand,
decreased
While
LT
alter
production
reactive
oxygen
species
(ROS)
monocytes,
it
significantly
reduced
ROS
induced
phorbol
12-myristate
13-acetate
(PMA).
addition,
phagocytosis
E.
enhanced
survival
intracellular
Furthermore,
triggered
cytokines
IL-1β,
IL-6
TNF-α
well
chemokines
CCL-3
CXCL-8.
Together,
our
contrast
STa,
death
disrupt
functions,
potentially
acting
an
evasion
strategy
establish
infection.
Poultry Science,
Год журнала:
2025,
Номер
104(5), С. 105005 - 105005
Опубликована: Март 7, 2025
This
study
was
conducted
to
investigate
the
protective
effects
of
chlorogenic
acid
(CGA)
on
intestinal
health
in
broilers
challenged
with
avian
pathogenic
Escherichia
coli
(APEC).
One
hundred
and
eighty
one-day-old
male
broiler
chicks
were
divided
into
three
groups
six
replicates
ten
each
for
a
21-day
trial.
The
birds
control
APEC
fed
basal
diet,
while
CGA-treated
group
received
diet
supplemented
1000
mg/kg
CGA.
At
14
days,
CGA
administered
an
suspension
Compared
group,
incorporation
decreased
mortality
cecal
colonies
bacterially
(P
<
0.05).
Additionally,
reduced
relative
weight
heart,
liver,
kidney,
gizzard,
proventriculus,
intestine,
as
well
serum
triglyceride
level
alanine
aminotransferase
activity
APEC-challenged
Supplementing
concentrations
interferon-γ,
tumor
necrosis
factor-α,
interleukin-1β,
and/or
interleukin-6
serum,
duodenum,
jejunum,
ileum
presumably
through
inactivation
toll-like
receptor
4/myeloid
differentiation
factor
88
pathway
administration
diamine
oxidase
d-lactate
endotoxin
concentrations,
but
increased
ratio
between
villus
height
crypt
depth
duodenum
jejunum
APEC-infected
chickens,
accompanied
by
restored
expression
tight
junction
proteins
(claudin-1,
claudin-2,
occludin,
zonula
occludens-1)
genes
involved
apoptosis
(B
cell
lymphoma-2
associated
X
protein,
B
lymphoma-2,
cysteine-requiring
aspartate
protease
9)
superoxide
dismutase,
glutathione
peroxidase,
catalase
activities,
levels
mucosa,
inhibited
accumulation
malondialdehyde
possibly
via
activating
nuclear
factor-erythroid
2-related
factor-2/heme
oxygenase-1
results
suggested
that
alleviated
APEC-induced
damage
inhibiting
inflammation
oxidative
stress.
However,
its
potential
application
practical
poultry
production
is
contingent
upon
both
efficacy
cost-effectiveness.
Benzoic
acid
as
a
dietary
supplement
mitigates
inflammation
and
intestinal
injury
in
acute
enterotoxigenic
Escherichia
coli
-infected
mice
without
adverse
effects
healthy
mice.
Animals,
Год журнала:
2024,
Номер
14(16), С. 2405 - 2405
Опубликована: Авг. 19, 2024
The
study
was
designed
to
investigate
the
protective
effect
of
dietary
supplementation
with
coated
benzoic
acid
(CBA)
on
intestinal
barrier
function
in
weaned
pigs
challenged
enterotoxigenic
Animals,
Год журнала:
2024,
Номер
14(17), С. 2599 - 2599
Опубликована: Сен. 6, 2024
The
antioxidant
and
immune
systems
of
weaned
piglets
are
not
fully
mature
also
subjected
to
serious
stress
challenges
related
oxidative
inflammation.
Selenium
(Se)
is
an
essential
element
for
pigs,
with
documented
roles
encompassing
antioxidative
anti-inflammatory
properties
via
selenoproteins.
Sodium
selenite
Se-enriched
yeast
commonly
acknowledged
as
conventional
sources
Se
piglets.
In
the
past
decade,
several
novel
have
emerged
in
field
piglet
nutrition.
this
review,
we
will
initially
outline
historical
timeline
reported
Afterwards,
our
attention
turn
towards
nutritional
regulation
relation
aspects
healthy
Ultimately,
provide
a
detailed
review
highlighting
potential
emerging
alleviating
various
adverse
effects
faced
by
These
include
stress,
enterotoxigenic
Escherichia
coli
infection,
lipopolysaccharide-induced
inflammation,
heat
exposure
feed
mycotoxins.
output
emphasize
fundamental
importance
incorporating
diet
