The crosstalk between exosomal miRNA and ferroptosis: A narrative review

Biology of the Cell, Год журнала: 2025, Номер 117(1)

Опубликована: Янв. 1, 2025

Ferroptosis is a type of cell death that multiple mechanisms and pathways contribute to the positive negative regulation it. For example, increased levels reactive oxygen species (ROS) induce ferroptosis. ferroptosis unlike apoptosis, it not dependent on caspases, but iron. Exosomes are membrane-bound vesicles with size about 30 150 nm, contain various cellular components, including DNA, RNA, microRNAs (miRNAs), lipids, proteins, which genetically similar their cells origin. found in all bodily fluids, blood, saliva, urine. Cells often release exosomes after fusion membrane. They play an important role immune cell-cell communication. miRNAs, noncoding RNAs length 18 24 nucleotides, involved regulating gene expression transcription. Emerging data suggests exosomal miRNAs implicated pathophysiological cells, metastasis, drug resistance, death. In addition, functional studies have indicated can key modulation by Therefore, this review, given importance ferroptosis, we decided elucidate relationship between diseases.

Язык: Английский

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microRNAs and their Therapeutic Strategy in Phase I and Phase II Clinical Trials

Epigenomics, Год журнала: 2024, Номер 16(4), С. 259 - 271

Опубликована: Фев. 1, 2024

miRNAs play a crucial therapeutic role in diseases such as cancer, diabetes and viral infections, with around 1900 identified the human genome. Some have progressed to clinical trials, miRNA mimics inhibitors are pivotal molecules undergoing evaluation. The review delves into various miRNA-associated emphasizing their precision targeting specific genes, modulating disease pathways diagnostic potential. This underscores importance of therapy, foreseeing innovations medicine techniques for diverse diseases. future envisions improved delivery systems addressing challenges like immunogenicity digestion, while comprehensive miRNA-based omics database could guide development tailored antisense miRNAs, further advancing strategies.

Язык: Английский

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PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway

World Journal of Surgical Oncology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 5, 2025

Renal cell carcinoma (RCC) is a frequent and aggressive type of kidney cancer with limited therapeutic options. Although phosphatase tensin homolog (PTEN) have been recognized as potential tumor suppressor in all kinds cancers, its function RCC remains to be thoroughly elucidated. This article was recruited examine the PTEN's role managing PI3K/AKT pathway impact on proliferation migration. study collected renal adjacent non-cancerous tissue samples from our hospital. HK-2 786-O cells were used, divided into control, vector, oe-PTEN groups. PTEN related protein levels detected using RT-qPCR Western blot. Statistical analyses performed Mann-Whitney U test Kruskal-Wallis H test. Cell viability migration assessed CCK-8 assay wound healing assay. All conducted SPSS 22.0 software, statistical significance defined p < 0.05. results showed that expression significantly increased tissues compared normal (p 0.01). However, mRNA reduced In low expression, further induction overexpression inhibited signaling activity 0.01), accompanied by decreased ability. These indicate pattern complex, but can exert tumor-suppressive effects inhibiting pathway. Our findings demonstrate leads signaling, decreasing highlights critical progression suggests targets for intervention.

Язык: Английский

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Targeting Regulation of Macrophage to Treat Metabolic Disease: Role of Phytochemicals

Cell Proliferation, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Metabolic syndrome encompasses a cluster of predictive metabolic risk factors, including obesity, insulin resistance, dyslipidemia, hyperglycemia and hypertension. It is strongly associated with the development type 2 diabetes cardiovascular disease. Given increasing morbidity mortality syndrome, along limited availability drug treatments, it high time to investigate pathogenesis this condition explore potential pharmacotherapies. Macrophages, well-known innate immune cells, play an essential role in maintaining tissue homeostasis multiple physiological processes, glucose lipid metabolism, oxidative stress inflammation. Emerging evidence indicates that effects macrophages are linked macrophage-mediated metaflammation. Phytochemicals derived from natural plants have been shown exert therapeutic on by modulating macrophage function. In review, we sort out metaflammation summarise phytochemicals target for treatment condition.

Язык: Английский

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MYLK-AS1 improves fracture by targeting miR-146a-5p to regulate cell viability and apoptosis in osteoblasts

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Март 19, 2025

Delayed fracture healing (DFH) is a significant burden for patients. Therefore, early diagnosis and detection are important the treatment of DFH. The long non-coding RNA (LncRNA) MYLK-AS1 abnormally expressed in patients with DFH has potential to be used as diagnostic marker. 40 87 normal were included. levels MYLK-AS1, miR-146a-5p several mRNA markers osteogenic differentiation assessed by RT-qPCR. value was using ROC curves. Cell proliferation ability CCK-8, apoptosis rate detected flow cytometry. DLR, RIP pull down assays demonstrated targeting relationship between miR-146a-5p. significantly lower up-regulated compared found target miR-146a-5p, negatively correlated each other. high High expression could inhibit levels, support cell proliferation, reduce apoptosis, increase osteogenesis-specific matrix proteins osteogenesis-related regulatory factors. marker By increasing level cells can activity osteoblasts their rate, thus affecting process healing.

Язык: Английский

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Correlation between thyroid hormone levels and the incidence and staging of bladder cancer

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Март 28, 2025

Given the role of thyroid hormones (THs) in metabolism, growth and development, their involvement carcinogenesis cancer progression context bladder (BC) warrants further investigation. This study aimed to investigate associations between TH levels incidence stage BC. A cohort 46 diagnosed BC patients with no history disease, 47 healthy controls were analysed. classified into NMIBC MIBC according 2017 TNM staging system (AJCC, 8th edition). Thyroid antibodies measured. Statistical analysis was performed via SPSS software evaluate differences parameters controls, non-muscle invasive (NMIBC) muscle (MIBC) patients. Compared had higher thyrotropin (TSH), total triiodothyronine (TT3), thyroxine (TT4) thyroglobulin lower peroxidase antibody (TPOAb). both elevated TT3 TT4 levels. The proportions significantly greater high TSH, TT3, groups than low groups. Elevated TPOAb within normal range appear be associated increased These findings suggest that may useful for assessing prognosis provide new insights therapeutic strategies.

Язык: Английский

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Effect of miR-654-3p targeting EMP1 on osteoblast activity and differentiation in delayed fracture healing

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Март 28, 2025

Язык: Английский

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Wighteone, a prenylated flavonoid from licorice, inhibits growth of SW480 colorectal cancer cells by allosteric inhibition of Akt

Journal of Ethnopharmacology, Год журнала: 2024, Номер 330, С. 118195 - 118195

Опубликована: Апрель 18, 2024

Язык: Английский

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Single nucleotide polymorphisms in ovarian cancer impacting lipid metabolism and prognosis: an integrated TCGA database analysis

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Март 13, 2025

Ovarian cancer (OC) stands as a formidable adversary among women, remaining leading cause of cancer-related mortality owing to its aggressive and invasive nature. Investigating prognostic markers intricately linked OC's molecular pathogenesis represents critical avenue for enhancing patient outcomes survival prospects. In this comprehensive study, we embarked on bioinformatics journey, leveraging the vast repository single nucleotide polymorphism (SNP) data from OC patients available within TCGA database. Our overarching goal was unearth genetic underpinnings OC, shedding light potential that could significantly impact clinical decision-making care. meticulous analysis led discovery five mutated genes—APOB, BRCA1, COL6A3, LRP1, LRP1B—engaged in intricate world lipid metabolism. These genes, previously unexplored context emerged prominent figures our investigation, showcasing their roles progression. The interplay between metabolism development has garnered considerable attention recent years, findings underscore relevance these genes OC. To fortify discoveries, delved into realm analysis, pivotal component investigation. results yielded compelling evidence significant correlations expression levels aforementioned genes. This insight underscores utility markers, illuminating path toward more personalized effective approaches study multifaceted approach unraveling complex By harnessing power high-throughput mining, uncovered insights may reshape understanding disease. We complemented with advanced techniques such RT-qPCR Western blot, further dissecting intricacies landscape. holistic not only deepens but also provides essential information holds promise assessing prognosis. summary, stride quest decode ovarian cancer. spotlight significance APOB, LRP1B, inviting exploration Ultimately, research carries shape future management, offering glimpse

Язык: Английский

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Serum exosomal miRNA contributes to the diagnosis of leptomeningeal carcinomatosis

Journal of Neuro-Oncology, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Leptomeningeal carcinomatosis (LC) is a severe complication in the advanced stage of lung adenocarcinoma, with an extremely poor prognosis. Currently, diagnosis LC poses challenges. Serum exosomal miRNAs (microRNAs) have been demonstrated to possess potential as viable biomarkers. However, their value remains unclear. In this study, serum samples were collected from adenocarcinoma patients LC. The control groups consisted early-stage and advanced-stage without exosomes isolated for high - throughput RNA sequencing screen differential miRNAs, results validated 123 samples. Subsequently, receiver operating characteristic (ROC) curve was used evaluate diagnostic ability miRNA revealed seven differentially enriched (miRNA-1296-5p, miR-503-5p, miR-499a-5p, miR-374a-5p, miR-3173-5p, miR-370-3p miR-885-3p). ddPCR confirmed that expression levels miRNA-1296-5p, miR-499a-5p miR-374a-5p significantly elevated LC, while decreased (P < 0.05). ROC analysis showed AUC combination CEA 0.803 0.0001), displaying higher power This study suggests specific which has potential. And can further enhance

Язык: Английский

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