Rapid Detection of Npy Methylation in Colorectal Cancer Using a Singlet Oxygen-Based Photoelectrochemical Ratiometric Bio-Platform
Опубликована: Янв. 1, 2025
Язык: Английский
Single Methylation Sensitive Restriction Endonuclease-Based Cascade Exponential Amplification Assay for Visual Detection of DNA Methylation at Single-Molecule Level
Analytical Chemistry,
Год журнала:
2024,
Номер
96(32), С. 13335 - 13343
Опубликована: Авг. 2, 2024
Function
as
a
potential
cancer
biomarker,
DNA
methylation
shows
great
significance
in
diagnosis,
prognosis,
and
treatment
monitoring.
While
the
lack
of
an
ultrasensitive,
specific,
accurate
method
at
single-molecule
level
hinders
analysis
exceedingly
low
levels
methylation.
Herein,
based
on
outstanding
recognition
digestion
ability
methylation-sensitive
restriction
endonuclease
(MSRE),
we
established
single
MSRE-based
cascade
exponential
amplification
method,
which
requires
only
two
ingeniously
designed
primers
one
site
MSRE
for
detection
Differentiated
by
digestion,
cleaved
unmethylated
is
too
short
to
induce
any
reactions,
while
methylated
remains
intact
trigger
subsequent
CRISPR/Cas12a
system.
By
integrating
1
aM
can
be
accurately
detected,
corresponds
6
molecules
10
μL
system,
indicating
that
our
more
sensitive
than
amplification-based
methods
with
detect
level.
In
addition,
0.1%
effectively
distinguished
from
large
amounts
DNA.
Our
further
introduced
exploit
expression
difference
among
normal
cells
cells.
Moreover,
visual
also
realized
full
hybridization
between
products
crRNA
CRISPR/Cas12a.
Therefore,
proposed
has
promising
robust
bisulfite-free
level,
importance
early
diagnosis
cancer.
Язык: Английский
An introduction to personalized medicine
Therapeutic Drug Monitoring,
Год журнала:
2024,
Номер
unknown, С. 331 - 354
Опубликована: Янв. 1, 2024
Pharmacogenetic
testing
(PGx),
a
necessary
component
of
personalized
medicine,
is
powerful
tool
to
assist
in
prescribing
safe
and
efficacious
medications
individual
patients.
PGx
utilizes
the
information
provided
by
an
individual's
unique
genetic
makeup
potentially
predict
both
pharmacokinetic
profiles
pharmacodynamic
response
for
increasing
number
xenobiotics.
This
chapter
will
provide
broad
overview
pharmacogenetic
approaches.
More
recent
pharmacogenomics
approaches
discoveries,
such
as
genome-wide
association
studies
pathway
analyses
candidate
genes,
also
be
discussed.
comment
on
challenges
advantages
integrating
part
medicine
philosophy,
which
advocates
rational,
individualized
pharmacotherapy.
Язык: Английский
Current data and future perspectives on DNA methylation in ovarian cancer (Review)
International Journal of Oncology,
Год журнала:
2024,
Номер
64(6)
Опубликована: Май 8, 2024
Ovarian
cancer
(OC)
represents
the
most
prevalent
malignancy
of
female
reproductive
system.
Its
distinguishing
features
include
a
high
aggressiveness,
substantial
morbidity
and
mortality,
lack
apparent
symptoms,
which
collectively
pose
significant
challenges
for
early
detection.
Given
that
aberrant
DNA
methylation
events
leading
to
altered
gene
expression
are
characteristic
numerous
tumor
types,
there
has
been
extensive
research
into
epigenetic
mechanisms,
particularly
methylation,
in
human
cancers.
In
context
OC,
is
often
associated
with
regulation
critical
genes,
such
as
BRCA1/2
Ras‑association
domain
family
1A.
Methylation
modifications
within
promoter
regions
these
genes
not
only
contribute
pathogenesis
but
also
induce
medication
resistance
influence
prognosis
patients
OC.
As
such,
more
in‑depth
understanding
underpinning
carcinogenesis
could
potentially
facilitate
development
effective
therapeutic
approaches
this
intricate
disease.
The
present
review
focuses
on
classical
suppressor
oncogenes,
signaling
pathways
microRNAs
an
aim
elucidate
progression
advantages
limitations
employing
diagnosis,
treatment
prevention
OC
discussed.
On
whole,
literature
indicates
specific
serve
prognostic
biomarker
target
personalized
strategies.
Further
investigations
field
may
yield
efficacious
diagnostic
alternatives
Язык: Английский