BRAF-Mutated Melanoma Cell Lines Develop Distinct Molecular Signatures After Prolonged Exposure to AZ628 or Dabrafenib: Potential Benefits of the Antiretroviral Treatments Cabotegravir or Doravirine on BRAF-Inhibitor-Resistant Cells DOI Open Access
Valentina Zanrè, Francesco Bellinato,

Alessia Cardile

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 11939 - 11939

Опубликована: Ноя. 6, 2024

Melanoma is an aggressive cancer characterized by rapid growth, early metastasis, and poor prognosis, with resistance to current therapies being a significant issue. BRAF mutations drive uncontrolled cell division activating the MAPK pathway. In this study, A375 FO-1, BRAF-mutated melanoma lines, were treated for 4-5 months RAF inhibitor dabrafenib or AZ628, leading drug over time. The resistant cells showed altered molecular signatures, differences in cycle regulation propensity of death. Dabrafenib-resistant maintained high proliferative activity, while AZ628-resistant cells, especially exhibited slow-cycling, senescent-like phenotype susceptibility ferroptosis, form death driven iron. Antiretroviral drugs doravirine cabotegravir, known their effects on human endogenous retroviruses, tested impact these cells. Both reduced viability colony formation lines. Doravirine was particularly effective reactivating apoptosis reducing growth highly increasing tumor-suppressor proteins p16

Язык: Английский

BRAF-Mutated Melanoma Cell Lines Develop Distinct Molecular Signatures After Prolonged Exposure to AZ628 or Dabrafenib: Potential Benefits of the Antiretroviral Treatments Cabotegravir or Doravirine on BRAF-Inhibitor-Resistant Cells DOI Open Access
Valentina Zanrè, Francesco Bellinato,

Alessia Cardile

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 11939 - 11939

Опубликована: Ноя. 6, 2024

Melanoma is an aggressive cancer characterized by rapid growth, early metastasis, and poor prognosis, with resistance to current therapies being a significant issue. BRAF mutations drive uncontrolled cell division activating the MAPK pathway. In this study, A375 FO-1, BRAF-mutated melanoma lines, were treated for 4-5 months RAF inhibitor dabrafenib or AZ628, leading drug over time. The resistant cells showed altered molecular signatures, differences in cycle regulation propensity of death. Dabrafenib-resistant maintained high proliferative activity, while AZ628-resistant cells, especially exhibited slow-cycling, senescent-like phenotype susceptibility ferroptosis, form death driven iron. Antiretroviral drugs doravirine cabotegravir, known their effects on human endogenous retroviruses, tested impact these cells. Both reduced viability colony formation lines. Doravirine was particularly effective reactivating apoptosis reducing growth highly increasing tumor-suppressor proteins p16

Язык: Английский

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