Activation of GPER1 alleviates white matter injury by promoting microglia M2 polarization through EGFR/Stat3 pathway in intracerebral hemorrhage mice DOI Creative Commons
Xuyang Zhang, Jianchao Mao, Huanhuan Li

и другие.

Journal of Stroke and Cerebrovascular Diseases, Год журнала: 2025, Номер 34(6), С. 108315 - 108315

Опубликована: Апрель 12, 2025

White matter injury (WMI) is a major pathophysiological process after intracerebral hemorrhage (ICH). G protein-coupled estrogen receptor 1 (GPER1) has been validated to exert crucial role in regulating neuroinflammation and microglia polarization. Our previous report reveals activation of GPER1 improves the neurological deficits ICH via inhibition A1 astrocytes. However, on protection WMI modulation polarization remains unclear. In present study, mice model was induced by autologous whole blood injection vitro established treatment BV2 cells with FeSO4. Mice were treated agonist G1, antagonist G15 or EGFR inhibitor AG1478. Besides, conditional medium used intervene MO3.13 oligodendrocytes. Immunostaining, immunoblots, transmission electron microscope PI staining determine WMI, potential molecular mechanism ICH, respectively. data showed G1 ameliorated day 3 ICH. reduced release IL-1β, TNF-α increased produce IL-4, IL-10 as well shifting from proinflammatory M1 anti-inflammatory M2 phenotype vivo vitro. Meanwhile, alleviated oligodendrocytes death mitigating modulating Mechanistic study demonstrated EGFR/Stat3 signaling pathway involved Collectively, our findings through pathway.

Язык: Английский

Extracellular cold-inducible RNA-binding protein in CNS injury: molecular insights and therapeutic approaches DOI Creative Commons

Dmitriy Lapin,

Archna Sharma, Ping Wang

и другие.

Journal of Neuroinflammation, Год журнала: 2025, Номер 22(1)

Опубликована: Янв. 21, 2025

Abstract Central nervous system (CNS) injuries, such as ischemic stroke (IS), intracerebral hemorrhage (ICH) and traumatic brain injury (TBI), are a significant global burden. The complex pathophysiology of CNS is comprised primary secondary injury. Inflammatory incited by damage-associated molecular patterns (DAMPs) which signal variety resident cells infiltrating immune cells. Extracellular cold-inducible RNA-binding protein (eCIRP) DAMP acts through multiple non-immune to promote inflammation. Despite the well-established role eCIRP in systemic sterile inflammation, its less elucidated. Recent literature suggests that pleiotropic inflammatory mediator also being evaluated clinical biomarker indicate prognosis injuries. This review provides broad overview injury, with focus on immune-mediated neuroinflammation. We then what known about mechanisms both non-CNS cells, identifying opportunities for further study. explore eCIRP’s potential prognostic marker severity outcome. Next, we provide an eCIRP-targeting therapeutics suggest strategies develop these agents ameliorate Finally, emphasize exploring novel mechanisms, aside from neuroinflammation, critical therapeutic target

Язык: Английский

Процитировано

2

Therapeutic Role of Microglia/Macrophage Polarization in Intracerebral Hemorrhage DOI Creative Commons
Raşit Dinç, Nurittin Ardıç

Clinical and Translational Neuroscience, Год журнала: 2025, Номер 9(1), С. 4 - 4

Опубликована: Янв. 20, 2025

Intracerebral hemorrhage (ICH) is a significant health problem with high mortality and morbidity rates, partly due to limited treatment options. Hematoma after ICH causes neurological deficits the mass effect. Hemorrhage catalyzes secondary damage, resulting in increased poor prognosis, problems. This review evaluates role of immunotherapeutic approaches based on original full-text articles pathophysiology immunotherapy ICH, emphasis modulation microglia/macrophage polarization M2 subtype. In this review, we concluded that injury progression complex multifaceted. Inflammation plays dominant injuries. Furthermore, cells involved inflammatory process have dual roles pro-inflammatory/destructive anti-inflammatory/healing. While inflammation makes immune system therapeutic target them can modulate Resident microglia (and even macrophages migrating from peripheral source) are important targets for because their initiation phase shaping immunity. Although clinical results remain poor, experimental trial data seem promising deciphering providing

Язык: Английский

Процитировано

0

Identification of AIF1 as a protein target of notoginsenosides in brain tissue of mice with intracerebral hemorrhage based on hapten immunoaffinity fishing technique DOI Creative Commons
Feiyan Chen, Wei Qin,

Qianlin Li

и другие.

Journal of Ginseng Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

A prospective cohort study on serum PINK1 as a biochemical marker in relation to poor neurological prognosis, stroke-associated pneumonia and early neurological deterioration after acute intracerebral hemorrhage DOI
Wen‐Fei Ni,

Chen Si-hua,

Le Cao Dai

и другие.

Clinica Chimica Acta, Год журнала: 2025, Номер unknown, С. 120282 - 120282

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Activation of GPER1 alleviates white matter injury by promoting microglia M2 polarization through EGFR/Stat3 pathway in intracerebral hemorrhage mice DOI Creative Commons
Xuyang Zhang, Jianchao Mao, Huanhuan Li

и другие.

Journal of Stroke and Cerebrovascular Diseases, Год журнала: 2025, Номер 34(6), С. 108315 - 108315

Опубликована: Апрель 12, 2025

White matter injury (WMI) is a major pathophysiological process after intracerebral hemorrhage (ICH). G protein-coupled estrogen receptor 1 (GPER1) has been validated to exert crucial role in regulating neuroinflammation and microglia polarization. Our previous report reveals activation of GPER1 improves the neurological deficits ICH via inhibition A1 astrocytes. However, on protection WMI modulation polarization remains unclear. In present study, mice model was induced by autologous whole blood injection vitro established treatment BV2 cells with FeSO4. Mice were treated agonist G1, antagonist G15 or EGFR inhibitor AG1478. Besides, conditional medium used intervene MO3.13 oligodendrocytes. Immunostaining, immunoblots, transmission electron microscope PI staining determine WMI, potential molecular mechanism ICH, respectively. data showed G1 ameliorated day 3 ICH. reduced release IL-1β, TNF-α increased produce IL-4, IL-10 as well shifting from proinflammatory M1 anti-inflammatory M2 phenotype vivo vitro. Meanwhile, alleviated oligodendrocytes death mitigating modulating Mechanistic study demonstrated EGFR/Stat3 signaling pathway involved Collectively, our findings through pathway.

Язык: Английский

Процитировано

0