Опубликована: Янв. 1, 2024
Язык: Английский
Current Opinion in Genetics & Development, Год журнала: 2025, Номер 92, С. 102347 - 102347
Опубликована: Апрель 14, 2025
Язык: Английский
Процитировано
0
Insects, Год журнала: 2023, Номер 14(8), С. 706 - 706
Опубликована: Авг. 14, 2023
Autophagy is a process that promotes the lysosomal degradation of cytoplasmic proteins and highly conserved in eukaryotic organisms. maintains homeostasis organisms regulates multiple developmental processes, autophagy disruption related to human diseases. However, functional roles mediating innate immune responses are largely unknown. In this study, we sought understand how Atg2, an autophagy-related gene, functions immunity Drosophila melanogaster. The results showed large number melanotic nodules were produced upon inhibition Atg2. addition, inhibiting Atg2 suppressed phagocytosis latex beads, Staphylococcus aureus Escherichia coli; proportion Nimrod C1 (one receptors)-positive hemocytes also decreased. Moreover, altered actin cytoskeleton patterns, showing longer filopodia but with decreased numbers filopodia. expression AMP-encoding genes was by Drosomycin upregulated, transcript levels Attacin-A, Diptericin Metchnikowin Finally, above alterations caused prevented flies from resisting invading pathogens, low susceptible Erwinia carotovora 15 infections. conclusion, regulated both cellular humoral Drosophila. We have identified as crucial regulator immunity, which further established interactions between immunity.
Язык: Английский
Процитировано
4
Cells, Год журнала: 2024, Номер 13(14), С. 1191 - 1191
Опубликована: Июль 14, 2024
Autophagy is a degradative recycling process central to the maintenance of homeostasis in all eukaryotes. By ensuring degradation damaged mitochondria, it plays key role maintaining mitochondrial health and function. Of highly conserved autophagy proteins, autophagy-related protein 1 (Atg1) essential process. The involvement these proteins intracellular signalling pathways, including those involving function, are still being elucidated. Here Atg1 was investigated simple model organism Dictyostelium discoideum using an atg1 null mutant mutants overexpressing or antisense-inhibiting atg1. When evaluated against well-characterised outcomes dysfunction this model, altered expression resulted unconventional set phenotypic growth, endocytosis, multicellular development, homeostasis. findings here show that involved tightly regulated signal transduction pathway coordinating energy-consuming processes such as cell growth along with nutrient status energy production. Furthermore, Atg1’s effects on indicate peripheral ancillary network, balance rather than direct electron transport chain Further research required tease out complex networks. Nevertheless, study adds further evidence theory not opposing but linked, yet strictly controlled, homeostatic mechanisms.
Язык: Английский
Процитировано
1
Glia, Год журнала: 2023, Номер 72(2), С. 433 - 451
Опубликована: Окт. 23, 2023
Abstract Mitochondria support the energetic demands of cells. Autophagic turnover mitochondria serves as a critical pathway for mitochondrial homeostasis. It is unclear how bioenergetics and autophagy are functionally connected. Here, we identify an endolysosomal membrane protein that facilitates to regulate ATP production in glia. We determined Drosophila tweety ( tty ) highly expressed glia localized endolysosomes. Diminished fusion between autophagosomes endolysosomes ‐deficient was rescued by expressing human Tweety Homolog 1 TTYH1 ). Loss attenuated turnover, elevated oxidative stress, impaired locomotor functions. The cellular organismal defects were partially reversed antioxidant treatment. performed live‐cell imaging genetically encoded metabolite sensors determine impact deficiencies on glial bioenergetics. found exhibited reduced pyruvate consumption accompanied shift toward glycolysis production. Likewise, genetic inhibition resulted similar glycolytic Furthermore, survival mutant flies became more sensitive starvation, underlining significance crosstalk Together, our findings uncover role homeostasis via facilitating autophagy, which determines bioenergetic balance
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Окт. 22, 2024
Abstract Autophagy is a cytoprotective mechanism responsible for the maintenance and long-term survival of various cell types, including stem cells. However, its role in Germline (GSC)-niche, effects on GSCs niche cells, remains unexplored. We demonstrate that autophagy flux female Drosophila low dependent core gene, Atg5 . Atg5-/- within GSC-niche was unaffected. In contrast, disruption cap cells (niche cells) leads to loss both during aging. Further, reduced severely impairs crucial GSC self-renewal signal mediated by BMP-pMad emanating from post-midlife. essential Our study reveals differential autophagy, which dispensable but necessary where it supports signaling maintain GSCs.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0