Acta Chimica Sinica, Год журнала: 2025, Номер 83(4), С. 428 - 428
Опубликована: Янв. 1, 2025
Язык: Английский
Life Sciences, Год журнала: 2024, Номер 350, С. 122747 - 122747
Опубликована: Май 24, 2024
Язык: Английский
Процитировано
4
Journal of Agriculture and Food Research, Год журнала: 2024, Номер unknown, С. 101628 - 101628
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
3
Tissue and Cell, Год журнала: 2025, Номер 93, С. 102749 - 102749
Опубликована: Янв. 23, 2025
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1058 - 1058
Опубликована: Янв. 26, 2025
Probiotic bacteria are normal inhabitants of a healthy human gut, conferring multiple beneficial effects on the gut and beyond. Under various disease states, abundance diversity significantly decreased, process called dysbiosis. Among intra- extracellular components probiotics, vesicles (EVs) secreted by them have recently garnered significant attention as potential mediators probiotics’ host health. Further, these nanosized particles that encapsulate wide range bioactive molecules (proteins, lipids, RNA, DNA) standing out key factors could mediate microbiota–host communication confer ameliorating in experimental inflammatory, metabolic, cardiovascular models. However, standard protocol EV isolation from probiotic bacteria, not varying lab to lab, must be established achieve consistency results pre-clinical Our current study compared two commonly used methods for biological samples, ultracentrifugation precipitation, develop isolating EVs probiotics Lactobacillus acidophilus (LA), Gram-positive bacterium, Escherichia coli Nissle (EcN), Gram-negative bacterium. The method gave ~1.5-fold higher yield both LA EcN precipitation method. released level LA. were quantified characterized nanoparticle-tracking analysis (NTA) measuring specific surface biomarkers using Western blot. Here, we describe our standardized step-by-step their characterization.
Язык: Английский
Процитировано
0
Journal of Controlled Release, Год журнала: 2025, Номер 379, С. 951 - 966
Опубликована: Фев. 3, 2025
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 25 - 56
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Frontiers in Medicine, Год журнала: 2025, Номер 12
Опубликована: Март 13, 2025
Exosomes, nanosized extracellular vesicles released by various cell types, are intensively studied for the diagnosis and treatment of cancer neurodegenerative diseases, they also display high usability in regenerative medicine. Emphasizing their diagnostic potential, exosomes serve as carriers disease-specific biomarkers, enabling non-invasive early detection personalized The cargo loading with therapeutic agents presents an innovative strategy targeted drug delivery, minimizing off-target effects optimizing interventions. In medicine, play a crucial role intercellular communication, facilitating tissue regeneration through transmission bioactive molecules. While acknowledging existing challenges standardization scalability, ongoing research efforts aim to refine methodologies address regulatory considerations. summary, this review underscores transformative potential reshaping landscape medical interventions, particular emphasis on cancer,
Язык: Английский
Процитировано
0
Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 20
Опубликована: Март 14, 2025
Downstream processing continues to face significant bottlenecks due current purification technologies and improvements in upstream. Chromatography systems have been the primary method for their high yields purities. However, use of high-titer-producing strains has highlighted limitations chromatographic steps, including mass transfer limitations, low capacity, scalability issues. These challenges, combined with growing interest fully continuous manufacturing processes, led a widespread alternative affinity chromatography systems. Polyethylene glycol precipitation demonstrated be powerful, flexible, easily scalable, titer-independent methodology purifying therapeutic proteins such as monoclonal antibodies, achieving purities comparable Furthermore, it also holds great potential simplifying processes new modalities overcome downstream processing. Herein, we discuss latest advances polyethylene technology explore its future research directions applications.
Язык: Английский
Процитировано
0
Food Chemistry X, Год журнала: 2025, Номер unknown, С. 102395 - 102395
Опубликована: Март 1, 2025
This study aimed to separate and purify antifreeze peptides (AFP) from the autolysate of Litopenaeus vannamei head, peptide sequences with activity were identified elucidate potential mechanisms. The initial fractionation revealed that pk1 fraction less than 3 kDa molecular weight exhibited highest thermal hysteresis (2.28 °C). Notably, component pk1-A strongest (2.80 °C) was further separated by using a Sephadex G-15 gel filtration column. results bioinformatics computer-assisted techniques indicated 26 types as AFP. Among these, KQVHPDTGISSK selected head (LvAFP). active site (Lys residue) LvAFP discovered strongly interact water molecules via hydrogen bonding, thereby inhibiting formation recrystallization ice crystals. Therefore, preparation could improve high-value utilization shrimp byproducts.
Язык: Английский
Процитировано
0
World Journal of Gastrointestinal Oncology, Год журнала: 2025, Номер 17(4)
Опубликована: Март 24, 2025
Gastrointestinal (GI) cancers, which predominantly manifest in the stomach, colorectum, liver, esophagus, and pancreas, accounting for approximately 35% of global cancer-related mortality. The advent liquid biopsy has introduced a pivotal diagnostic modality early identification premalignant GI lesions incipient cancers. This non-invasive technique not only facilitates prompt therapeutic intervention, but also serves as critical adjunct prognosticating likelihood tumor recurrence. wealth circulating exosomes present body fluids is often enriched with proteins, lipids, microRNAs, other RNAs derived from cells. These specific cargo components are reflective processes involved tumorigenesis, progression, response to treatment. As such, they represent group promising biomarkers aiding diagnosis cancer. In this review, we delivered an exhaustive overview composition pathways sorting within these vesicles. We laid out some clinical evidence that supported utilization cancers discussed their potential application. Furthermore, addressed challenges encountered when harnessing predictive instruments realm
Язык: Английский
Процитировано
0