Acta Chimica Sinica, Год журнала: 2025, Номер 83(4), С. 428 - 428
Опубликована: Янв. 1, 2025
Язык: Английский
Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106844 - 106844
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 25, 2025
Extracellular vesicles (EVs) play crucial roles in intercellular communication and hold great promise as biomarkers for noninvasive disease diagnosis. Intensive research efforts have been devoted to discovering the EV subpopulations responsible specific functions or with enhanced effectiveness markers, through extensive purification content analysis. However, their high heterogeneity size cargo composition poses significant challenges reaching such goals. Isolation methods like ultracentrifugation size-exclusion chromatography, well analysis approaches polymerase chain reaction enzyme-linked immunosorbent assay, made contributions improving our understanding of biology. Nonetheless, these face limitations isolation efficiency, purity, detection sensitivity specificity due issues large sample consumption, unsatisfactory insufficient resolution subtyping. Microfluidic technology presents promising solutions challenges, leveraging intrinsic capabilities precise flow external energy field manipulation, compartmentalization, signal enhancement at micro- nanoscale. Hence, this review summarizes recent developments microfluidics-enabled analysis, paying special attention unique microfluidic features exploited. Strategies viscoelastic inertial flow, fluid mixing, external-field-assisted purification, compartmentalization micro/nanostructures enhancing detection, are examined. Furthermore, current potential future directions discussed inspire advancements rapidly developing field.
Язык: Английский
Процитировано
0
Talanta, Год журнала: 2025, Номер unknown, С. 128055 - 128055
Опубликована: Апрель 1, 2025
In this study, we investigated the behavior of extracellular vesicles (EVs), during capillary isoelectric focusing (cIEF). For that, used different approaches, imaging cIEF with a whole-column detection (WCID) and conventional as well methods (LIF after EV labelling, native fluorescence UV). Our study reveals that EVs exhibit significant aggregation their migration toward, upon reaching, point (pI). By optimizing key parameters such voltage addition solubilizers, successfully reduced issue, particularly bovine milk EVs. findings also showed distinct pI regions observed for isolated from sources: shows acidic characteristics (4.0-4.1), while pig human plasma more basic zones (4.7-4.9 5.8-6.7, respectively). The was extended to coupled laser induced (LIF) using intra-vesicular CFDA-labeled EVs, better understand susceptibilities. Prolonged mobilization time due long lengths adversely affected EV's integrity in cIEF. necessity specific optimization each source variations charge distribution across regions. use short length (<10 cm), low electric field solubilizers Tween-20 is recommended preserve cIEF-EV studies.
Язык: Английский
Процитировано
0
Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114675 - 114675
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Acta Chimica Sinica, Год журнала: 2025, Номер 83(4), С. 428 - 428
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0