Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells DOI Creative Commons
Yi Wang, Huabin Zhu, Shanjiang Zhao

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 19, 2024

Abstract Background: Negative energy balance (NEB) typically occurs in dairy cows after delivery, with those high yielding being more likely to experience significant NEB. Such metabolic imbalance could cause ketosis, which is often accompanied by a decline reproductive performance, but the molecular mechanism has not been fully elucidated. During excessive NEB, body fat extensively broken down, resulting abnormal accumulation of non-esterified fatty acids (NEFA), represented palmitic acid (PA), within uterus. an potential damage bovine endometrial epithelial cells (BEECs), while mechanisms underlying its involvement PA-induced injury BEECs poorly understood. Melatonin (MT) recognized for regulatory role maintaining homeostasis mitochondrial reactive oxygen species (mitoROS). However, less known about whether MT ameliorate PA on and mechanism. Results: Our results demonstrated that 0.2 mM stress increased level cellular oxidative stress. Further, we observed dysfunction, including incidence structure respiratory function, reduction membrane copy number, turn induced apoptosis. Notably, also found upregulation autophagy protein (PINK, Parkin, LC3B et al), however, P62 was increased. As expected, 100uM pre-treatment attenuated ROS restored function. Meanwhile, pre-treated reversed activate AMPK-mTOR-Beclin-1 pathway, contributing increase Conclusions: These findings indicate induce dysfunction enhance BEECs. In addition, hypothesized only reduce facilitate clearance damaged mitochondria through regulation pathways, thereby safeguarding pool promoting viability. study helps better understand involved NEB's impact fertility outcomes cows.

Язык: Английский

Protective effect of melatonin against metabolic disorders and neuropsychiatric injuries in type 2 diabetes mellitus mice DOI
Xinran Gao,

Huaizhi Sun,

Yadong Wei

и другие.

Phytomedicine, Год журнала: 2024, Номер 131, С. 155805 - 155805

Опубликована: Июнь 4, 2024

Язык: Английский

Процитировано

8
Melatonin alleviates retinal injury induced by vigabatrin and partially enhances its antiepileptic effects DOI

Meng-Die Ma,

Min Fan, Songlin Xu

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 153, С. 114516 - 114516

Опубликована: Март 29, 2025

Язык: Английский

Процитировано

0
Insufficient Plasma Melatonin and Its Association With Neuropsychiatric Impairments in Patients With T2DM DOI Creative Commons
Shuai He, Yue Yu,

Peng-quan Chen

и другие.

Journal of Diabetes Research, Год журнала: 2024, Номер 2024(1)

Опубликована: Янв. 1, 2024

Purpose: Type 2 diabetes mellitus (T2DM) is associated with multiple neuropsychiatric impairments, including cognitive dysfunction, and melatonin (MLT) plays a crucial role in maintaining normal functions. This study aimed at investigating the change plasma MLT levels its association impairments T2DM patients. Methods: One hundred twenty‐six patients were recruited, their demographics clinical data collected. Apart from glycated hemoglobin (HbA1c) other routine metabolic indicators, concentrations of MLT, C‐reactive protein (CRP), Interleukin 6 (IL‐6), soluble myeloid triggered receptor 1 (sTREM 1), 2) measured. Moreover, executive function depressive tendency evaluated via Behavior Rating Inventory Executive Function‐Adult Version (BRIEF‐A) Epidemiological Research Center Depression Scale (CES‐D), respectively. Result: Compared low HbA1c group, high group presented lower but higher inflammatory biomarker levels, together scores BRIEF‐A CES‐D scales. results Pearson correlation test showed that negatively correlated scores, as well indications, indicating may mediate neuroinflammation impairments. Furthermore, ROC curve indicated have predictive effect on impairment status Conclusion: decreased status, might be developed therapeutic agent indicator for T2DM‐associated depression status.

Язык: Английский

Процитировано

3
Optimizing melatonin dosage for neuroprotection in HT22 hippocampal neuronal cells DOI Open Access
Abair Awadalla Ahmed Mahdi, Abubaker Y. Elamin, Hala Mahgoub Hamour

и другие.

Annals of Phytomedicine An International Journal, Год журнала: 2024, Номер 13(1)

Опубликована: Июль 1, 2024

Melatonin is widely studied for its neuroprotective properties in various cellular models, but the optimal dosage therapeutic effects remains poorly defined, especially neuronal cell lines like HT22.Discrepancies literature regarding effective melatonin concentrations necessitate a systematic investigation to establish standardized window that balances efficacy and safety.The aim identify of promotes viability neuroprotection HT22 hippocampal line over exposure times.A quantitative analysis using MTT assay measured cells treated with increasing (0.1 mM 5 mM) time intervals 24, 48, 72 h.This approach allowed assessment both cytotoxic across range dosages.The study revealed dose-dependent impact on viability, higher leading significant effects.Cell was relatively high at lower 0.5 all points showed marked decline exceeding 0.75 mM.The balance between minimal cytotoxicity found doses from 0.1 0.25 half-maximal inhibitory concentration (IC 50 ) values indicated increased sensitivity time, decreasing 0.83 24 h 0.39 successfully defines more precise cells, suggesting optimize without inducing effects.These findings contribute ongoing effort standardize dosages neurobiological research clinical applications, potentially influencing.

Язык: Английский

Процитировано

2
Melatonin alleviates palmitic acid-induced mitochondrial dysfunction by reducing oxidative stress and enhancing autophagy in bovine endometrial epithelial cells DOI Creative Commons
Yi Wang,

Jianfei Gong,

Nuo Heng

и другие.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 8, 2024

Negative energy balance (NEB) typically occurs in dairy cows after delivery. Cows with a high yield are more likely to experience significant NEB. This type of metabolic imbalance could cause ketosis, which is often accompanied by decline reproductive performance. However, the molecular mechanisms underlying NEB have yet be fully elucidated. During excessive NEB, body fat extensively broken down, resulting abnormal accumulation non-esterified fatty acids (NEFAs), represented palmitic acid (PA), within uterus. Such an has potential damage bovine endometrial epithelial cells (BEECs), while its involvement PA-induced injury BEECs remains poorly understood. Melatonin (MT) recognized for regulatory role maintaining homeostasis mitochondrial reactive oxygen species (mitoROS). little known as whether MT ameliorate incurred response PA and mechanism involved.

Язык: Английский

Процитировано

1
Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells DOI Creative Commons
Yi Wang, Huabin Zhu, Shanjiang Zhao

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 19, 2024

Abstract Background: Negative energy balance (NEB) typically occurs in dairy cows after delivery, with those high yielding being more likely to experience significant NEB. Such metabolic imbalance could cause ketosis, which is often accompanied by a decline reproductive performance, but the molecular mechanism has not been fully elucidated. During excessive NEB, body fat extensively broken down, resulting abnormal accumulation of non-esterified fatty acids (NEFA), represented palmitic acid (PA), within uterus. an potential damage bovine endometrial epithelial cells (BEECs), while mechanisms underlying its involvement PA-induced injury BEECs poorly understood. Melatonin (MT) recognized for regulatory role maintaining homeostasis mitochondrial reactive oxygen species (mitoROS). However, less known about whether MT ameliorate PA on and mechanism. Results: Our results demonstrated that 0.2 mM stress increased level cellular oxidative stress. Further, we observed dysfunction, including incidence structure respiratory function, reduction membrane copy number, turn induced apoptosis. Notably, also found upregulation autophagy protein (PINK, Parkin, LC3B et al), however, P62 was increased. As expected, 100uM pre-treatment attenuated ROS restored function. Meanwhile, pre-treated reversed activate AMPK-mTOR-Beclin-1 pathway, contributing increase Conclusions: These findings indicate induce dysfunction enhance BEECs. In addition, hypothesized only reduce facilitate clearance damaged mitochondria through regulation pathways, thereby safeguarding pool promoting viability. study helps better understand involved NEB's impact fertility outcomes cows.

Язык: Английский

Процитировано

0