Structural and Functional Adaptive Artificial Bone: Materials, Fabrications, and Properties

Advanced Functional Materials, Год журнала: 2023, Номер 33(23)

Опубликована: Март 6, 2023

Abstract It is an urgent need that defect repair can develop from simple device fixation to living tissue reconstruction, short life function replacement permanent regeneration repair. At present, bone transplantation has become the second largest surgery after blood transfusion, and artificial generates great hope for treatment of defect. In order defect, must have good biological properties sufficient mechanical properties, it should also shape matching site connected porous structure. For structures requirements bone, in this review three major challenges faced by are systemtically analyzed current methods strategies address these issues discussed: 1) developing a type scaffold material with both 2) realizing controllable fabrication individual multistage pore structure scaffold, 3) transformation man‐made Besides, summarizes advantages disadvantages discusses potential future directions structural functional adaptive regeneration.

Язык: Английский

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Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

Advanced Functional Materials, Год журнала: 2022, Номер 32(20)

Опубликована: Фев. 9, 2022

Abstract Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking with hierarchical micro/nanofibrous microchannel structures is still challenge. Herein, inspired by structural functional cues remodeling, networks‐enriched nanofibrous using printing thermally induced phase separation techniques, which facilitate cells migration nutrients transportation, developed. The customizable vascular‐like structure polycaprolactone the gelatin‐silica fabricated 3D‐printed sacrificial templates, while dimethyloxalylglycine (DMOG)‐loaded mesoporous silica nanoparticles (MSNs) located on surface forming peptide‐1 (BFP)‐loaded MSNs embedded in are implemented for sequential release DMOG BFP. cell experiments show that dual‐drug delivery (DBM/GP) promotes angiogenesis stimulating migration, tube formation, angiogenesis‐related genes/protein expression endothelial cells, osteogenesis promoting osteo‐related genes mineral deposition osteoblasts. Additionally, DBM/GP facilitates angiogenic activity osteoblasts activating phosphatidylinositol 3‐kinase/protein kinase B/hypoxia inducible factor‐1α pathway. Furthermore, enhanced vascularization regeneration demonstrated via subcutaneous skull defect models. Overall, this study reveals microenvironment‐mimetic provides promising strategy defects treatment.

Язык: Английский

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Flowerbed-Inspired Biomimetic Scaffold with Rapid Internal Tissue Infiltration and Vascularization Capacity for Bone Repair

ACS Nano, Год журнала: 2023, Номер 17(5), С. 5140 - 5156

Опубликована: Фев. 21, 2023

The favorable microstructure and bioactivity of tissue-engineered bone scaffolds are closely associated with the regenerative efficacy defects. For treatment large defects, however, most them fail to meet requirements such as adequate mechanical strength, highly porous structure, excellent angiogenic osteogenic activities. Herein, inspired by characteristics a "flowerbed", we construct short nanofiber aggregates-enriched dual-factor delivery scaffold via 3D printing electrospinning techniques for guiding vascularized regeneration. By assembly nanofibers containing dimethyloxalylglycine (DMOG)-loaded mesoporous silica nanoparticles printed strontium-contained hydroxyapatite/polycaprolactone (SrHA@PCL) scaffold, an adjustable structure can be easily realized changing density nanofibers, while strong compressive strength will acquired due framework role SrHA@PCL. Owing different degradation performance between electrospun microfilaments, sequential release behavior DMOG Sr ions is achieved. Both in vivo vitro results demonstrate that has biocompatibility, significantly promotes angiogenesis osteogenesis stimulating endothelial cells osteoblasts, effectively accelerates tissue ingrowth regeneration through activating hypoxia inducible factor-1α pathway immunoregulatory effect. Overall, this study provided promising strategy constructing microenvironment-matched biomimetic

Язык: Английский

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3D bioprinted gelatin/gellan gum-based scaffold with double-crosslinking network for vascularized bone regeneration

Carbohydrate Polymers, Год журнала: 2022, Номер 290, С. 119469 - 119469

Опубликована: Апрель 9, 2022

Язык: Английский

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Metal‐Phenolic Nanocapsules with Photothermal Antibacterial and Ros Scavenging Ability for Diabetic Wound Healing

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(10)

Опубликована: Янв. 2, 2024

The presence of bacteria in diabetic wounds not only leads to the formation biofilms but also triggers oxidative stress and inflammatory responses, which hinder wound-healing process. Therefore, it is imperative formulate a comprehensive strategy that can proficiently eliminate enhance wound microenvironment. Herein, this work develops multifunctional metal-phenolic nanozymes (TA-Fe/Cu nanocapsules), wherein one-pot coordination tannic acid (TA)and Fe

Язык: Английский

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A mechanical-assisted post-bioprinting strategy for challenging bone defects repair

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 26, 2024

Bioprinting that can synchronously deposit cells and biomaterials has lent fresh impetus to the field of tissue regeneration. However, unavoidable occurrence cell damage during fabrication process intrinsically poor mechanical stability bioprinted cell-laden scaffolds severely restrict their utilization. As such, on basis heart-inspired hollow hydrogel-based (HHSs), a mechanical-assisted post-bioprinting strategy is proposed load into HHSs in rapid, uniform, precise friendly manner. show responsiveness within 4 s, 13-fold increase number, partitioned loading two types compared with those under static conditions. proof concept, an enhanced regenerative capability repair critical-sized segmental osteoporotic bone defects vivo. We expect this provide universal, efficient, promising way promote cell-based therapy.

Язык: Английский

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Revolutionizing Bone Regeneration: Vascularized Bone Tissue Engineering with Advanced 3D Printing Technology

Aggregate, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

ABSTRACT The repair and functional reconstruction of bone defects resulting from trauma, surgical resection, degenerative diseases, congenital malformations are major clinical challenges. Bone tissue engineering has significant advantages in the treatment severe defects. Vascularized scaffolds gradually attracting attention development because their excellent biomimetic properties efficient efficiency. Three‐dimensional (3D) printing technology, which can be used to fabricate structures at different scales using a wide range materials, been production vascularized scaffolds. This review discusses research progress 3D for Angiogenesis‐osteogenesis coupling regeneration process is first introduced, followed by summary technologies, inks, bioactive factors Notably, this focuses on structural design strategies Finally, application medicine, as well challenges outlooks future development, described.

Язык: Английский

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Engineered mammalian and bacterial extracellular vesicles as promising nanocarriers for targeted therapy

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2022, Номер 3(1), С. 63 - 86

Опубликована: Янв. 1, 2022

Extracellular vesicles (EVs), which are nanocarriers with phospholipid bilayer structures released by most cells, play a key role in regulating physiological and pathological processes. EVs have been investigated due to their loading capacity, low toxicity, immunogenicity, biofunctions. Although shown good potential as therapeutic vehicles, natural poor targeting ability, substantially reduces the effect. Through addition of unit into membrane surface or inside engineering technology, agent can accumulate specific cells tissues. Here, we focus on mammalian (MEVs) bacterial (BEVs), two common types biomedical field. In this review, describe engineered MEVs BEVs promising for targeted therapy summarize biogenesis, isolation, characterization BEVs. We then techniques enhancement ability EVs. Moreover, applications therapy, including treatment cancer brain bone disease. believe that review will help improve understanding BEVs, thereby promoting application clinical translation.

Язык: Английский

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Dissolving microneedle-encapsulated drug-loaded nanoparticles and recombinant humanized collagen type III for the treatment of chronic wound via anti-inflammation and enhanced cell proliferation and angiogenesis

Nanoscale, Год журнала: 2022, Номер 14(4), С. 1285 - 1295

Опубликована: Янв. 1, 2022

Nowadays, diabetic chronic wounds impose a heavy burden on patients and the medical system. Persistent inflammation poor tissue remodeling severely limit healing of wounds. For these issues, first recombinant humanized collagen type III (rhCol III) naproxen (Nap) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticle incorporated hyaluronic acid (HA) microneedle (MN) was fabricated for wound therapy. As tailored rhCol synthesized based Gly483-Pro512 segment, which contained highly adhesive fragments (GER, GEK) in human sequence, it possessed strong cell adhesion. The mechanical strength prepared MN enough to overcome barrier necrosis/hyperkeratosis minimally invasive way after being applied Subsequently, Nap@PLGA nanoparticles were rapidly released site within few minutes. favourable biocompatibility could effectively facilitate proliferation migration fibroblasts endothelial cells. Furthermore, regenerative efficacy evaluated vivo using rat full-thickness skin model. These results illustrated that accelerate closure by reducing inflammatory response enhancing angiogenesis or deposition, indicating their significant application value dressings repair.

Язык: Английский

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Stat3 Signaling Pathway: A Future Therapeutic Target for Bone-Related Diseases

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Апрель 25, 2022

Signal transducer and activator of transcription 3 (Stat3) is activated by phosphorylation translocated to the nucleus participate in transcriptional regulation DNA. Increasing evidences point that aberrant activation or deletion Stat3 plays a critical role broad range pathological processes including immune escape, tumorigenesis, inflammation. In bone microenvironment, acts as common downstream response protein for multiple cytokines engaged modulation cellular proliferation intercellular interactions. has direct impacts on disease progression regulating mesenchymal stem cells differentiation, osteoclast activation, macrophage polarization, angiogenesis, cartilage degradation. Here, we describe theoretical basis key roles different bone-related diseases combination with vitro experiments animal models. Then, summarize categorize drugs target Stat3, providing potential therapeutic strategies their use diseases. conclusion, could be future

Язык: Английский

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