Advanced Healthcare Materials,
Год журнала:
2022,
Номер
11(13)
Опубликована: Март 20, 2022
Various
scaffolding
systems
have
been
attempted
to
facilitate
vascularization
in
tissue
engineering
by
optimizing
biophysical
properties
(e.g.,
vascular-like
structures,
porous
architectures,
surface
topographies)
or
loading
biochemical
factors
growth
factors,
hormones).
However,
during
ossification
remains
an
unmet
challenge
that
hampers
the
repair
of
large
bone
defects.
In
this
study,
reconstructing
vascularized
bones
situ
against
critical-sized
defects
is
endeavored
using
newly
developed
scaffolds
made
chemically
cross-linked
gelatin
microsphere
aggregates
(C-GMSs).
The
rationale
design
lies
creation
and
optimization
cell-material
interfaces
enhance
focal
adhesion,
proliferation,
function
anchorage-dependent
functional
cells.
vitro
trials
are
carried
out
coculturing
human
aortic
endothelial
cells
(HAECs)
murine
osteoblast
precursor
(MC3T3-E1)
within
C-GMS
scaffolds,
which
endothelialized
bone-like
constructs
yielded.
Angiogenesis
osteogenesis
induced
C-GMSs
scaffold
further
confirmed
via
subcutaneous-embedding
nude
mice.
for
femoral
subsequently
performed
rats.
acellular
with
interconnected
macropores,
exhibit
capability
recruit
endogenous
bone-forming
cells,
vascular
forming
immunocytes)
then
promote
regeneration
as
well
integration
host
bone.
Biomaterials Research,
Год журнала:
2022,
Номер
26(1)
Опубликована: Апрель 28, 2022
Abstract
Background
Even
though
the
modulatory
effects
of
Magnisum
(Mg)
and
its
alloys
on
bone-healing
cells
have
been
widely
investigated
during
last
two
decades,
relatively
limited
attention
has
paid
their
inflammation-modulatory
properties.
Understanding
activation
process
macrophages
in
response
to
dynamic
degradation
Mg
as
well
relationship
between
macrophage
phenotypes
osteogenic
potential
is
critical
for
design
development
advanced
Mg-based
or
Mg-incorporated
biomaterials.
Methods
In
this
work,
a
Ti-0.625
(wt.%)
alloy
fabricated
by
mechanical
alloying
(MA)
subsequent
spark
plasma
sintering
(SPS)
was
employed
material
model
explore
inflammatory
performance
vitro
vivo
taking
pure
Ti
control.
The
data
analysis
performed
following
Student’s
t-test.
Results
results
revealed
that
grown
underwent
sequential
M1
M2
culture
period
5
days.
initially
increased
environmental
pH
(~
8.03)
responsible
macrophages,
while
accumulated
2+
within
contributed
lateral
phenotype
activation.
Both
promoted
osteoblast-like
SaOS-2
cell
maturation.
experiment
further
showed
better
anti-inflammatory
response,
regenerative
potentiality
thinner
fibrous
tissue
layer
than
Ti.
Conclusion
highlighted
roles
importance
modulating
M1-to-M2
transition
Biomedical Materials,
Год журнала:
2022,
Номер
17(2), С. 025006 - 025006
Опубликована: Янв. 7, 2022
Abstract
Vascularization
of
thick
hydrogel
scaffolds
is
still
a
big
challenge,
because
the
submicron-
or
nano-sized
pores
seriously
restrict
endothelial
cells
adhesion,
proliferation
and
migration.
Therefore,
porous
hydrogels
have
been
fabricated
as
kind
promising
hydrous
for
enhancing
vascularization
during
tissue
repairing.
In
order
to
investigate
effects
pore
size
on
vascularization,
macroporous
methacrylated
hyaluronic
acid
(HAMA)
with
different
sizes
were
by
gelatin
microspheres
(GMS)
template
method.
After
leaching
out
GMS
templates,
uniform
highly
interconnected
macropores
formed
in
hydrogels,
which
provided
an
ideal
physical
microenvironment
induce
human
umbilical
vein
(HUVECs)
migration
vascularization.
vitro
results
revealed
that
facilitated
compared
non-macroporous
hydrogels.
Hydrogels
middle
200–250
μm
(HAMA250
hydrogels)
supported
best
cell
furthest
3D
HUVECs.
The
influences
then
evaluated
subcutaneous
embedding.
vivo
illustrated
HAMA250
exhibited
optimum
behavior.
Highest
number
newly
blood
vessels
expression
CD31
could
be
found
rather
than
other
summary,
our
concluded
was
μm.
This
research
provides
new
insight
into
engineering
vascularized
tissues
may
find
utility
designing
regenerative
biomaterial
scaffolds.
Acta Biomaterialia,
Год журнала:
2022,
Номер
156, С. 250 - 268
Опубликована: Авг. 28, 2022
The
development
of
tissue
engineering
strategies
for
treatment
large
bone
defects
has
become
increasingly
relevant,
given
the
growing
demand
substitutes.
Native
is
composed
a
dense
vascular
network
necessary
regulation
development,
regeneration
and
homeostasis.
A
major
obstacle
in
fabricating
living,
clinically
relevant-sized
mimics
(1-10
cm3)
limited
supply
nutrients,
including
oxygen
to
core
construct.
Therefore,
support
vascularization
are
pivotal
engineered
constructs.
Creating
functional
construct
integrated
with
network,
capable
delivering
nutrients
optimal
imperative
translation
into
clinics.
system
complex
that
runs
throughout
body
tree-like
hierarchical
branching
fashion.
significant
challenge
approaches
lies
mimicking
intricate,
multi-scale
structures
consisting
larger
vessels
(macro-vessels)
which
interconnect
multiple
sprouting
(microvessels)
closed
network.
advent
biofabrication
enabled
complex,
out
plane
channels
be
generated
laid
groundwork
creation
vasculature
recent
years.
This
review
highlights
key
state-of-the-art
achievements
networks
varying
scales
field
particular
focus
its
application
developing
There
need
substitutes
overcome
patient-derived
bone.
Bone
aims
this
by
combining
stem
cells
scaffolds
restore
missing
current
bottleneck
upscaling
lack
an
required
delivery
cells.
3D
bioprinting
techniques
hollow
dimensions
resemble
native
blood
vessels.
convergence
materials,
cell
types
fabrication
approaches,
opens
possibility
clinically-relevant
sized
vascularized
provides
up-to-date
insight
technologies
currently
available
generation
networks,
on
their
engineering.
Advanced Healthcare Materials,
Год журнала:
2022,
Номер
11(13)
Опубликована: Март 20, 2022
Various
scaffolding
systems
have
been
attempted
to
facilitate
vascularization
in
tissue
engineering
by
optimizing
biophysical
properties
(e.g.,
vascular-like
structures,
porous
architectures,
surface
topographies)
or
loading
biochemical
factors
growth
factors,
hormones).
However,
during
ossification
remains
an
unmet
challenge
that
hampers
the
repair
of
large
bone
defects.
In
this
study,
reconstructing
vascularized
bones
situ
against
critical-sized
defects
is
endeavored
using
newly
developed
scaffolds
made
chemically
cross-linked
gelatin
microsphere
aggregates
(C-GMSs).
The
rationale
design
lies
creation
and
optimization
cell-material
interfaces
enhance
focal
adhesion,
proliferation,
function
anchorage-dependent
functional
cells.
vitro
trials
are
carried
out
coculturing
human
aortic
endothelial
cells
(HAECs)
murine
osteoblast
precursor
(MC3T3-E1)
within
C-GMS
scaffolds,
which
endothelialized
bone-like
constructs
yielded.
Angiogenesis
osteogenesis
induced
C-GMSs
scaffold
further
confirmed
via
subcutaneous-embedding
nude
mice.
for
femoral
subsequently
performed
rats.
acellular
with
interconnected
macropores,
exhibit
capability
recruit
endogenous
bone-forming
cells,
vascular
forming
immunocytes)
then
promote
regeneration
as
well
integration
host
bone.
