The Potential of Siraitia grosvenorii to Promote Bone Regeneration via Modulating Macrophage Polarization: A Network Pharmacology and Experimental Study DOI Open Access

Yuying Mai,

Linhui Huang, Qiao Yang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3609 - 3609

Опубликована: Апрель 11, 2025

Siraitia grosvenorii (SG), a traditional Chinese medicinal herb, possesses immunomodulatory and osteoinductive properties, yet its pharmacological mechanisms in bone defect repair remain largely unexplored. This study investigates the therapeutic potential of SG through combination network pharmacology experimental approaches. Active compounds were identified using Traditional Medicine Systems Pharmacology (TCMSP) Platform, protein interaction targets predicted. Molecular docking dynamics simulations assessed interactions between critical targets. In vitro, RAW 264.7 macrophages treated with SG-conditioned medium exhibited enhanced M2 polarization reduced inflammation, promoting osteogenic differentiation co-cultured MC3T3-E1 cells as evidenced by increased alkaline phosphatase activity. vivo, scaffolds loaded low-dose or high-dose (LSG/HSG) significantly improved regeneration rat calvarial defects, HSG achieving near-complete mature trabeculae at 8 weeks, alongside decreased CD86 TNF-α levels IL-10 expression. Network 33 shared related to macrophage polarization, kaempferol beta-sitosterol demonstrating strong binding affinities such TNF, PTGS2, CASP3. These findings highlight enhancing implications for regenerative medicine.

Язык: Английский

Natural, safety immunomodulatory derivatives of lactobacillus biofilms promote diabetic wound healing by metabolically regulating macrophage phenotype and alleviating local inflammation DOI Creative Commons

Qingwei Zhou,

Junjie Chi, Jintao Yang

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Long-term inflammatory microenvironment further impairs the healing process of diabetic wounds. Many studies have shown that Lactobacillus can regulate immune function and promote injured tissue repair. However, immunomodulatory safety biofilm (LB) on wounds need investigation. In this present research, we proposed a "bacteria-free derivative therapy" successfully extracted derivatives (LBDs) by ultrasonic separation filtration technology for natural safe treatment The study first cultured anaerobically LBDs using ultrasound combined with technology. were characterized via scanning electron microscopy, Concanavalin A fluorescence staining, protein gel electrophoresis. vivo wound model, closure rates dynamically monitored, sections analyzed hematoxylin-eosin immunofluorescence staining to evaluate LBDs' effects. An in vitro macrophage inflammation model was established, employing immunofluorescence, flow cytometry, Western blotting techniques explore molecular mechanisms underlying effects phenotypes. Furthermore, whole-genome sequencing proteomics LBDs-treated macrophages performed elucidate intrinsic through which effectively utilizing coupled Studies revealed modulate systemic metabolic reprogramming wound-site macrophages, suppress JAK-STAT1 signaling pathway, alleviate local microenvironment, neovascularization ultimately accelerate healing. retains most bioactive components LB. As natural, agent, metabolically phenotypes improving offering promising potential regenerative applications management.

Язык: Английский

Процитировано

0
The Potential of Siraitia grosvenorii to Promote Bone Regeneration via Modulating Macrophage Polarization: A Network Pharmacology and Experimental Study DOI Open Access

Yuying Mai,

Linhui Huang, Qiao Yang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3609 - 3609

Опубликована: Апрель 11, 2025

Siraitia grosvenorii (SG), a traditional Chinese medicinal herb, possesses immunomodulatory and osteoinductive properties, yet its pharmacological mechanisms in bone defect repair remain largely unexplored. This study investigates the therapeutic potential of SG through combination network pharmacology experimental approaches. Active compounds were identified using Traditional Medicine Systems Pharmacology (TCMSP) Platform, protein interaction targets predicted. Molecular docking dynamics simulations assessed interactions between critical targets. In vitro, RAW 264.7 macrophages treated with SG-conditioned medium exhibited enhanced M2 polarization reduced inflammation, promoting osteogenic differentiation co-cultured MC3T3-E1 cells as evidenced by increased alkaline phosphatase activity. vivo, scaffolds loaded low-dose or high-dose (LSG/HSG) significantly improved regeneration rat calvarial defects, HSG achieving near-complete mature trabeculae at 8 weeks, alongside decreased CD86 TNF-α levels IL-10 expression. Network 33 shared related to macrophage polarization, kaempferol beta-sitosterol demonstrating strong binding affinities such TNF, PTGS2, CASP3. These findings highlight enhancing implications for regenerative medicine.

Язык: Английский

Процитировано

0