ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
10(9), С. 5513 - 5536
Опубликована: Авг. 22, 2024
Irreversible
bone
defects
resulting
from
trauma,
infection,
and
degenerative
illnesses
have
emerged
as
a
significant
health
concern.
Structurally
functionally
controllable
hydrogels
made
by
tissue
engineering
(BTE)
become
promising
biomaterials.
Natural
proteins
are
able
to
establish
connections
with
autologous
through
unique
biologically
active
regions.
Hydrogels
based
on
can
simulate
the
microenvironment
regulate
biological
behavior
of
stem
cells
in
niche,
making
them
candidates
for
research
related
regeneration.
This
article
reviews
functions
various
natural
macromolecular
(such
collagen,
gelatin,
fibrin,
silk
fibroin)
highlights
their
special
advantages
hydrogels.
Then
latest
trends
cross-linking
modified
protein
improved
mechanical
properties
composite
loaded
exogenous
micromolecular
been
discussed.
Finally,
applications
hydrogels,
such
3D
printed
microspheres,
injectable
were
introduced,
aiming
provide
reference
repair
clinical
defects.
Theranostics,
Год журнала:
2024,
Номер
14(15), С. 6016 - 6035
Опубликована: Янв. 1, 2024
Total
hip
arthroplasty
(THA)
and
total
knee
(TKA)
are
effective
interventions
for
end-stage
osteoarthritis;
however,
periprosthetic
infection
is
a
devastating
complication
of
arthroplasty.
To
safely
prevent
enhance
osteointegration,
the
surface
modification
strategy
was
utilized
to
kill
bacteria,
modulate
osteoimmune
microenvironment,
improve
new
bone
formation.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(34), С. 8420 - 8430
Опубликована: Янв. 1, 2024
The
engineered
surfaces,
linearly
aligned
triangular
micropatterns
(TPs)
with
small
interspaces,
promote
osteogenic
differentiation
in
BMSCs
via
Piezo1-mediated
myosin
remodeling
and
YAP1
nuclear
translocation.
Cells
were
enlarged
elongated.
Medicina,
Год журнала:
2025,
Номер
61(1), С. 76 - 76
Опубликована: Янв. 4, 2025
Background
and
Objectives:
Insulin-like
growth
factor-1
(IGF-1)
plays
a
vital
role
in
various
cellular
processes,
including
those
involving
stem
cells.
This
study
evaluated
the
effects
of
IGF-1
on
cell
survival,
osteogenic
differentiation,
mRNA
expression
gingiva-derived
mesenchymal
spheroids.
Materials
Methods:
Using
concave
microwells,
spheroids
were
generated
presence
at
concentrations
0,
10,
100
ng/mL.
Cellular
vitality
was
qualitatively
assessed
using
microscopy,
while
water-soluble
tetrazolium
salt–based
assay
kit
quantified
viability.
Osteogenic
differentiation
via
alkaline
phosphatase
activity
an
anthraquinone
dye
test
to
measure
calcium
deposition.
Additionally,
quantitative
polymerase
chain
reaction
(qPCR)
analysis
performed
determine
RUNX2
COL1A1.
Results:
By
day
1,
had
successfully
formed,
their
morphology
remained
stable
over
following
7
days.
The
tested
showed
no
significant
differences
Similarly,
revealed
observable
changes.
However,
7,
incorporation
led
increase
Alizarin
Red
staining,
indicative
enhanced
Notably,
concentration
ng/mL
significantly
upregulated
Conclusions:
These
findings
suggest
that
supports
maintenance
viability
promotes
COL1A1
spheroids,
highlighting
its
potential
enhancing
differentiation.
Future
research
should
include
long-term
studies
evaluate
sustainability
IGF-1-induced
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 24, 2025
Abstract
Spinal
cord
injury
(SCI)
leads
to
acute
tissue
damage
that
disrupts
the
microenvironmental
homeostasis
of
spinal
cord,
inhibiting
cell
survival
and
function,
thereby
undermining
treatment
efficacy.
Traditional
stem
therapies
have
limited
success
in
SCI,
due
difficulties
maintaining
inducing
sustained
differentiation
into
neural
lineages.
A
new
solution
may
arise
from
controlling
fate
cells
by
creating
an
appropriate
mechanical
microenvironment.
In
this
study,
response
complex
(MRSCC)
is
created
as
innovative
therapeutic
strategy
for
utilizing
3D
bioprinting
technology
gelatin
microcarriers
(GM)
loaded
with
mesenchymal
(MSCs).
GM
creates
optimal
microenvironment
MSCs
growth
paracrine
activity.
Meanwhile,
allows
accurate
control
spatial
pore
architecture
characteristics
construct
encourage
neuroregeneration.
The
MRSCC
found
activate
Piezo1
channel
prevent
excessive
nuclear
translocation
YAP,
increasing
neural‐related
gene
expression
MSCs.
Transplanting
rats
injuries
boosts
sensory
motor
recovery,
reduces
inflammation,
stimulates
regeneration
neurons
glial
cells.
offers
a
engineering
can
promote
repair.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
Abstract
A
multifunctional
bone
tissue
engineering
scaffold
combining
spontaneous
biomineralization
with
magnetically
induced
dynamic
mechanical
stimulation
is
developed
to
promote
stem
cell
osteogenic
differentiation
and
accelerate
matrix
formation.
Inspired
by
the
“rebar‐concrete”
structure,
a
composite
(PGS‐P@MGel)
combines
3D‐printed
phosphate‐modified
poly(glycerol
sebacate)
(PGS‐P)
hydrogel
containing
PDA@Fe
3
O
4
magnetic
nanoparticles
(MGel).
The
framework
provides
mineralization
structural
integrity,
accelerating
calcium
deposition
providing
stable
support.
injected
into
enables
controllable
signals
under
external
fields,
directing
fate.
Mechanotransduction
activates
Piezo1
pathway,
inducing
β‐catenin
YAP
overexpression
enhance
osteogenesis.
In
vitro/vivo
studies
demonstrate
this
functionalized
exhibits
excellent
biocompatibility,
promotes
angiogenesis,
enhances
efficiency,
demonstrates
superior
capacity
influence
of
an
field.
This
scaffold,
its
mechanoactive,
effectively
regulates
defect
repair,
opening
new
dimensions
for
development
scaffolds
valuable
insights
large‐scale
repair.
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 26, 2025
Abstract
Organoids
provide
3D
structures
that
replicate
native
tissues
in
biomedical
research.
The
development
of
vascular
networks
within
organoids
enables
oxygen
and
nutrient
delivery
while
facilitating
metabolic
waste
removal,
which
supports
organoid
growth
maturation.
Recent
studies
demonstrate
vascularized
models
offer
insights
into
tissue
interactions
promote
regeneration.
However,
the
current
limitations
establishing
functional
affect
growth,
viability,
clinical
translation
potential.
This
review
examines
organoids,
including
mechanisms
angiogenesis
vasculogenesis,
construction
strategies,
applications.
approaches
are
categorized
vivo
vitro
methods,
with
analysis
their
specific
advantages
limitations.
also
discusses
emerging
techniques
such
as
bioprinting
gene
editing
for
improving
vascularization
integration
organoid‐based
therapies.
Current
developments
indicate
potential
applications
modeling
human
diseases
developing
therapeutic
contributing
to
advances
translational
