Physical stimuli-responsive 3D printable hydrogels for scalable manufacturing of bioengineered meat analogs

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104867 - 104867

Опубликована: Янв. 1, 2025

Язык: Английский

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Amyloid Fibrils and Their Applications: Current Status and Latest Developments

Nanomaterials, Год журнала: 2025, Номер 15(4), С. 255 - 255

Опубликована: Фев. 7, 2025

Amyloid fibrils are one of the important forms protein aggregates, first discovered in pathological brain tissues patients with various neurodegenerative diseases. They considered core markers different In recent years, research has found that multiple proteins or peptides dynamically assemble to form functional amyloid-like nanofibrils under physiological conditions, exhibiting excellent mechanical properties, high environmental stability, and self-healing ability. Therefore, they have become a class biological nanomaterials development potential. This article systematically reviews latest progress preparation, functionalization, application engineering provides an outlook on possible future directions.

Язык: Английский

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Animal Bodies and Futures: Rethinking Ethical Implications of Cultivated Meat and Fish

Food Ethics, Год журнала: 2025, Номер 10(1)

Опубликована: Фев. 15, 2025

Язык: Английский

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The potential function of soy protein hydrolysate to induce myogenic differentiation of C2C12 cells

PLoS ONE, Год журнала: 2025, Номер 20(4), С. e0321650 - e0321650

Опубликована: Апрель 16, 2025

Muscle satellite cell (MSC) isolation, proliferation, and differentiation are the basis of cultured meat (CM) technology, which emerged as a sustainable moral substitute for conventional animal agriculture. Notwithstanding encouraging future CM, there still lot obstacles to overcome, like high expense culture media need fetal bovine serum (FBS). The goal this work is determine whether plant-based nitrogen source soy protein hydrolysate (SPH) can improve myogenic functional development in MSCs CM by acting substitute. We concentrated on how Angel Yeast Company’s SPH PU041 affected C2C12 mouse line, useful model studying muscle biology sector. Adding containing different concentrations FBS was found promote proliferation elongation, with optimal effects observed at 0.5 g/L. Immunofluorescence flow cytometry analyses revealed that up-regulated levels myosin heavy chain (MyHC) factor 1 (MyoD), key biomarkers myogenesis. Furthermore, quantitative real-time PCR (qPCR) confirmed up-regulation MyHC, MyoD, myogenin (MyoG) mRNA expression, indicating induces differentiation. findings suggest potentially be used reduce costs associated production viable substitute, thereby facilitating more ethical approach food production. However, precise mechanisms underlying PU041’s warrant further investigation.

Язык: Английский

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Notch Signaling in regulating Bone-derived Nanoparticles (BNPs) enhanced Osteogenic Differentiation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 22, 2024

Abstract Mesenchymal stem cells (MSCs)-based bone tissue regeneration has gained significant attention due to their excellent differentiation capacity and immunomodulatory activity. Enhancing osteogenesis regulation is crucial for improving the therapeutic efficacy of MSC- based regeneration. By utilizing regenerative ECM functionality nanoparticles, we recently engineered bone-based nanoparticles (BNPs) from decellularized porcine bone. The effects internalization BNPs on MSCs viability, proliferation, osteogenic were first investigated compared at different time points. phenotypic behaviors, including cell number, characterized compared. incorporating this LNA/DNA nanobiosensor live imaging, monitored Notch ligand delta-like 4 (Dll4) expression dynamics in cytoplasm nucleus during differentiation. Pharmacological interventions are used inhibit signaling examine mechanisms involved. results suggest inhibition mediates process, with reduced early late stage markers (ALP, calcium mineralization). led an increase Dll4 expression, exhibiting a time-dependent pattern that aligned enhanced proliferation Our findings indicate observed changes BNP-treated could be associated elevated levels mRNA expression. In summary, study provides new insights into molecular through which stimulate process. influence by modulating demonstrating potential link between proteins present BNPs.

Язык: Английский

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Editorial: Novel trends in cultured meat research

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Июль 16, 2024

Язык: Английский

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Bovine Placentome-Derived Extracellular Matrix: A Sustainable 3D Scaffold for Cultivated Meat

Bioengineering, Год журнала: 2024, Номер 11(8), С. 854 - 854

Опубликована: Авг. 21, 2024

Cultivated meat, an advancement in cellular agriculture, holds promise addressing environmental, ethical, and health challenges associated with traditional meat production. Utilizing tissue engineering principles, cultivated production employs biomaterials technologies to create cell-based structures by introducing cells into a biocompatible scaffold, mimicking organization. Among the cell sources used for producing muscle-like meats, primary adult stem like muscle satellite exhibit robust capabilities proliferation differentiation myocytes, presenting promising avenue Evolutionarily optimized growth 3D microenvironment, these benefit from biochemical biophysical cues provided extracellular matrix (ECM), regulating organization, interactions, behavior. While plant protein-based scaffolds have been explored their utilization they lack biological animal unless functionalized. Conversely, decellularized bovine placental ECM, processed discarded birth tissue, achieves functionalities of ECM without harming animals. In this study, collagen total were prepared tissues. The content was determined be approximately 70% 40% isolated respectively. resulting porous scaffolds, crosslinked through dehydrothermal (DHT) crosslinking method chemical agents, supported myoblasts. exhibited superior compatibility stability compared scaffolds. attempt make cultivate constructs, myoblasts cultured steak-shaped about 50 days. construct not only resembled tissues but also displayed high cellularity indications myogenic differentiation. Furthermore, constructs cookable able sustain grilling/frying. Our study is first utilize unique placentome-derived scaffold tissue-like construct, demonstrating sustainable option

Язык: Английский

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Animal-derived free hydrolysate in animal cell culture: Current research and application advances

Journal of Tissue Engineering, Год журнала: 2024, Номер 15

Опубликована: Янв. 1, 2024

Fetal bovine serum (FBS) plays a crucial role in the composition of animal cell culture medium. However, conventional serum-based medium face numerous challenges. The use animal-derived free hydrolysate (ADFH) has garnered significant attention research and applications as viable alternative to FBS-containing culture. This article provides comprehensive overview effects, mechanisms action, ADFH serves an effective substitute for medium, enhancing various cellular processes, including proliferation, viability, protein synthesis, production, survival, stability. Several action have been elucidated through scientific investigations, such nutrient provision, activation signaling pathways, regulation synthesis folding, protection against oxidative damage apoptosis, well cycle regulation. Researches represent promising approach overcoming limitations advancing field review theoretical foundation promoting development sustainable hydrolysates, continued progress

Язык: Английский

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Bone-derived Nanoparticles (BNPs) enhanced Osteogenic Differentiation via Notch Signaling

Nanoscale Advances, Год журнала: 2024, Номер unknown

Опубликована: Дек. 27, 2024

Bone-derived nanoparticles enhance osteogenic differentiation of mesenchymal stem cells through upregulating Dll4 expression.

Язык: Английский

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