Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach DOI Creative Commons
Mokgadi Ursula Makgobole, Stanley Chibuzor Onwubu, Abayomi Baruwa

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(12), С. 562 - 562

Опубликована: Дек. 17, 2024

Collagen, a critical biomaterial with wide applications in pharmaceuticals, cosmetics, and medical industries, can be sourced sustainably from fish scales. This study optimizes the extraction of collagen using Tris-Glycine buffer scales via Taguchi method. Various parameters—buffer concentration, temperature, pH, time—were evaluated to identify optimal conditions. Under conditions (0.5 M acetic acids, volume acids 100 mL, soaking time 120 min, 10 mL), results demonstrate that temperature concentration significantly influence yield, purity 17.14 ± 0.05 mg/g. R2 value 73.84% was obtained for mathematical model). FTIR analysis confirmed presence characteristic peaks at 1611 cm−1 (amide I), 1523 II), 1300 III), indicating successful type I collagen. SDS-PAGE revealed protein banding pattern consistent molecular weight collagen, amino acid shows high percentages glycine (20.98%), proline (15.43%), hydroxyproline (11.51%), implying fibrous structures. The finding suggests method offers an efficient sustainable approach extraction, reducing waste processing industries. Nevertheless, there is need further experimental validation align modeling on optimized

Язык: Английский

Innovation in Osteogenesis Activation: Role of Marine-Derived Materials in Bone Regeneration DOI Creative Commons
Maria Giovanna Rizzo, Marilena Briglia, Vincenzo Zammuto

и другие.

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 175 - 175

Опубликована: Март 7, 2025

Marine-derived biomaterials are emerging as promising candidates for tissue regeneration due to their sustainability, biocompatibility, bioactivity, and unique chemical structure. This review provides an overview of different marine-derived inorganic organic materials, such calcium carbonate, magnesium salts, silica, polysaccharides, bioactive peptides, lipid-based compounds, effects in promoting osteogenesis. Specifically, the osteoinductive, osteoconductive, osteointegrative activities traditional innovative materials that influence key molecular pathways BMP/Smad Wnt/β-catenin signaling underlying bone formation will be evaluated. also prospects approaches, i.e., phage display technology, optimize peptides targeted regeneration. In context sustainable this suggests some interesting applications unusual able overcome limitations conventional ones stimulate cellular by activating specific pathways.

Язык: Английский

Процитировано

0
Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach DOI Creative Commons
Mokgadi Ursula Makgobole, Stanley Chibuzor Onwubu, Abayomi Baruwa

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(12), С. 562 - 562

Опубликована: Дек. 17, 2024

Collagen, a critical biomaterial with wide applications in pharmaceuticals, cosmetics, and medical industries, can be sourced sustainably from fish scales. This study optimizes the extraction of collagen using Tris-Glycine buffer scales via Taguchi method. Various parameters—buffer concentration, temperature, pH, time—were evaluated to identify optimal conditions. Under conditions (0.5 M acetic acids, volume acids 100 mL, soaking time 120 min, 10 mL), results demonstrate that temperature concentration significantly influence yield, purity 17.14 ± 0.05 mg/g. R2 value 73.84% was obtained for mathematical model). FTIR analysis confirmed presence characteristic peaks at 1611 cm−1 (amide I), 1523 II), 1300 III), indicating successful type I collagen. SDS-PAGE revealed protein banding pattern consistent molecular weight collagen, amino acid shows high percentages glycine (20.98%), proline (15.43%), hydroxyproline (11.51%), implying fibrous structures. The finding suggests method offers an efficient sustainable approach extraction, reducing waste processing industries. Nevertheless, there is need further experimental validation align modeling on optimized

Язык: Английский

Процитировано

0