Menadione as Antibiotic Adjuvant Against P. aeruginosa: Mechanism of Action, Efficacy and Safety DOI Creative Commons
Kristela Shehu, Marc Schneider, Annette Kraegeloh

и другие.

Antibiotics, Год журнала: 2025, Номер 14(2), С. 163 - 163

Опубликована: Фев. 7, 2025

Background/Objectives: Antibiotic resistance in chronic lung infections caused by Pseudomonas aeruginosa requires alternative approaches to improve antibiotic efficacy. One promising approach is the use of adjuvant compounds that complement therapy. This study explores potential menadione as an azithromycin against planktonic cells and biofilms P. aeruginosa, focusing on its mechanisms action cytotoxicity pulmonary cell models. Methods: The effect improving antibacterial antibiofilm potency was tested aeruginosa. Mechanistic studies AZMr-E. coli DH5α were performed probe reactive oxygen species (ROS) production bacterial membrane disruption. Cytotoxicity concentrations assessed measuring ROS levels integrity Calu-3 A549 epithelial cells. Results: Adding 0.5 µg/mL reduced minimum inhibitory concentration (MIC) four-fold biofilm eradication (MBEC) two-fold Adjuvant involved disruption membranes. tests revealed (≤64 µg/mL) did not affect or lines. Conclusions: Menadione enhanced efficacy while exhibiting a favorable safety profile at concentrations. These findings suggest adjuvant. However, relevant data toxicity sparse, further are required ensure safe complementing

Язык: Английский

Menadione as Antibiotic Adjuvant Against P. aeruginosa: Mechanism of Action, Efficacy and Safety DOI Creative Commons
Kristela Shehu, Marc Schneider, Annette Kraegeloh

и другие.

Antibiotics, Год журнала: 2025, Номер 14(2), С. 163 - 163

Опубликована: Фев. 7, 2025

Background/Objectives: Antibiotic resistance in chronic lung infections caused by Pseudomonas aeruginosa requires alternative approaches to improve antibiotic efficacy. One promising approach is the use of adjuvant compounds that complement therapy. This study explores potential menadione as an azithromycin against planktonic cells and biofilms P. aeruginosa, focusing on its mechanisms action cytotoxicity pulmonary cell models. Methods: The effect improving antibacterial antibiofilm potency was tested aeruginosa. Mechanistic studies AZMr-E. coli DH5α were performed probe reactive oxygen species (ROS) production bacterial membrane disruption. Cytotoxicity concentrations assessed measuring ROS levels integrity Calu-3 A549 epithelial cells. Results: Adding 0.5 µg/mL reduced minimum inhibitory concentration (MIC) four-fold biofilm eradication (MBEC) two-fold Adjuvant involved disruption membranes. tests revealed (≤64 µg/mL) did not affect or lines. Conclusions: Menadione enhanced efficacy while exhibiting a favorable safety profile at concentrations. These findings suggest adjuvant. However, relevant data toxicity sparse, further are required ensure safe complementing

Язык: Английский

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