Insights into the Regulation of the Mitochondrial Inheritance and Trafficking Adaptor Protein Mmr1 in Saccharomyces cerevisiae DOI Creative Commons

Nourah Nayef,

Lakhan Ekal, Ewald H. Hettema

и другие.

Kinases and Phosphatases, Год журнала: 2024, Номер 2(2), С. 190 - 208

Опубликована: Июнь 18, 2024

Mitochondria are organelles involved in cellular energetics all eukaryotes, and changes their dynamics, fission, fusion, or localization can lead to cell defects disease humans. Budding yeast, Saccharomyces cerevisiae, has been shown be an effective model organism elucidating mechanisms underpinning these mitochondrial processes. In the work presented here, a genetic screen was performed identify overexpressing kinases, phosphatases, ubiquitin ligases, which resulted defects. A total of 33 overexpressed genes showed phenotypes but without severe growth These included subset that affected timing inheritance were focus further study. Using biochemical approaches, roles PAK-family kinase Cla4 E3-ubiquitin ligases Dma1 Dma2 investigated. Previous studies have indicated triggering degradation trafficking adaptors bud, leads disruption interaction with transporting class V myosin, Myo2. Here, we map key interface between adaptor Mmr1 necessary for phosphorylation region required its via Dma2. Together, our data provide insights into regulatory regions responsible function inheritance.

Язык: Английский

Dexmedetomidine alleviates intestinal ischemia/reperfusion injury by modulating intestinal neuron autophagy and mitochondrial homeostasis via Nupr1 regulation DOI Creative Commons
Qiong Wu, Qiuhong Chen,

Si-Si Liang

и другие.

Molecular Medicine, Год журнала: 2024, Номер 30(1)

Опубликована: Ноя. 6, 2024

Abstract Intestinal ischemia/reperfusion injury (I/R) is a common yet challenging-to-treat condition, presenting significant clinical challenge. This study aims to investigate the protective mechanisms of Dexmedetomidine (Dex) against I/R injury, with particular focus on its role in regulating autophagy activity intestinal neurons and maintaining mitochondrial homeostasis. Experimental findings demonstrate that Dex can mitigate damage induced by through modulation function suppressing expression Nupr1. discovery sheds light new molecular mechanism underlying potential efficacy treating offering valuable insights for therapy.

Язык: Английский

Процитировано

4

Deciphering the Molecular Pathways: How Polygonatum sibiricum Alleviates Myocardial Ischemia DOI Creative Commons

Zhanzhan Zhu,

Junzhi Pan,

Bin Jiang

и другие.

Journal of Food Biochemistry, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Herbal medicine, like Polygonatum sibiricum , is gaining attention for its potential in treating myocardial ischemia. Comprehensive research on molecular interplay essential development as a therapeutic agent. The aim to investigate the mechanisms by which ameliorates study involves thorough database exploration, docking, and use of both vivo vitro models detailed assessment. role HIF‐1α pathway particularly emphasized. Identification 11 principal components 23 core proteins, with dominant HIF‐1α, was achieved. A notable improvement conditions, attributable observed. improves ischemic injury augmenting mitochondrial activity enhancing pathway.

Язык: Английский

Процитировано

0

Effect of VBIT-4 on the functional activity of isolated mitochondria and cell viability DOI Creative Commons
Konstantin N. Belosludtsev,

Anna I Ilzorkina,

Lyudmila A Matveeva

и другие.

Biochimica et Biophysica Acta (BBA) - Biomembranes, Год журнала: 2024, Номер 1866(5), С. 184329 - 184329

Опубликована: Апрель 27, 2024

Язык: Английский

Процитировано

2

Insights into the Regulation of the Mitochondrial Inheritance and Trafficking Adaptor Protein Mmr1 in Saccharomyces cerevisiae DOI Creative Commons

Nourah Nayef,

Lakhan Ekal, Ewald H. Hettema

и другие.

Kinases and Phosphatases, Год журнала: 2024, Номер 2(2), С. 190 - 208

Опубликована: Июнь 18, 2024

Mitochondria are organelles involved in cellular energetics all eukaryotes, and changes their dynamics, fission, fusion, or localization can lead to cell defects disease humans. Budding yeast, Saccharomyces cerevisiae, has been shown be an effective model organism elucidating mechanisms underpinning these mitochondrial processes. In the work presented here, a genetic screen was performed identify overexpressing kinases, phosphatases, ubiquitin ligases, which resulted defects. A total of 33 overexpressed genes showed phenotypes but without severe growth These included subset that affected timing inheritance were focus further study. Using biochemical approaches, roles PAK-family kinase Cla4 E3-ubiquitin ligases Dma1 Dma2 investigated. Previous studies have indicated triggering degradation trafficking adaptors bud, leads disruption interaction with transporting class V myosin, Myo2. Here, we map key interface between adaptor Mmr1 necessary for phosphorylation region required its via Dma2. Together, our data provide insights into regulatory regions responsible function inheritance.

Язык: Английский

Процитировано

1