circDENND4C serves as a sponge for miR-200b to drive non-small cell lung cancer advancement by regulating MMP-9 expression

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Фев. 17, 2025

Introduction Lung cancer has a higher incidence and mortality rate than other cancers, especially non-small cell lung (NSCLC), accounting for 85% of the cases. The role circDENND4C/miR-200b/matrix metalloproteinase-9 (MMP-9) regulatory axis in NSCLC remains largely unknown. Methods lines were used to examine expression circDENND4C, miR-200b, MMP-9 via qRT-PCR or Western blot. target relationship was examined by RNA fluorescence situ hybridization (RNA-FISH), immunofluorescence (IF), dual-luciferase reporter system, quantitative real-time polymerase chain reaction (qRT-PCR), Then, count kit-8 (CCK-8) experiment, flow cytometry, migration/invasion assays performed assess biological function transfecting with their overexpression knockout plasmids A549 cells. Finally, proteins related adhesion tight junction further tested blot IF. Results circDENND4C found be highly expressed lines, while miR-200b lowly lines. Moreover, could sponge MMP-9. Subsequently, it observed that knockdown upregulation repressed proliferation cycle progression, increased apoptosis, hindered migration invasion. also circDENND4C/miR-200b/MMP-9 might involved influence tumor metastasis. Conclusions Altogether, our study reveals novel loop which may modulate indicating potential biomarkers diagnosis treatment NSCLC.

Язык: Английский

CircMALAT1 promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma via the miR-512-5p/VCAM1 axis

Acta Biochimica et Biophysica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Circular RNAs play a pivotal role in the progression of various cancers. In our previous study, we observed high expression circRNA MALAT1 (cMALAT1) intrahepatic cholangiocarcinoma (ICC) cells co-incubated with activated hepatic stellate cells. This study is designed to explore roles cMALAT1 and underlying mechanisms ICC. We find that significantly facilitates ICC both

Язык: Английский